Angiotensin-converting Enzyme Insertion/Deletion Polymorphism (rs4646994) and Susceptibility to Acute Lymphoblastic Leukemia: A Case–control Study

Al Romisa Ahmed Abdulaziz; Elshazali Widaa Ali

doi:10.3889/oamjms.2022.9341

Authors

Al Romisa Ahmed Abdulaziz Department of Haematology, Faculty of Medical Laboratory Science, Sudan University for Science and Technology, Khartoum, Sudan
Elshazali Widaa Ali Department of Medical Laboratory Science, College of Applied Medical Science, University of Bisha, Bisha, Saudi Arabia; Department of Haematology, Faculty of Medical Laboratory Sciences, Al Neelain University, Khartoum, Sudan https://orcid.org/0000-0003-2787-2055

DOI:

https://doi.org/10.3889/oamjms.2022.9341

Keywords:

Angiotensin-converting enzyme, Insertion/deletion polymorphism, Acute lymphoblastic leukemia

Abstract

BACKGROUND: Angiotensin-converting enzyme (ACE) stimulates the proliferation of bone marrow hematopoietic progenitors and thought to be involved in pathological neoplastic hematopoiesis and leukemogenesis.

AIM: This study aimed to investigate the association between ACE gene I/D polymorphism and the risk of acute lymphoblastic leukemia (ALL).

MATERIALS AND METHODS: A total of 96 subjects were recruited for the study, 48 patients with ALL, and 48 apparently healthy volunteers as a control group. Genomic DNA was extracted from peripheral leukocytes and ACE I/D polymorphism was analysed using allele-specific polymerase chain reaction.

RESULTS: In both study groups, the ACE D/D polymorphic genotype was the most frequent (52.1% and 54.2%, respectively), followed by the ID genotype (47.9% and 45.8% respectively), while the II genotype was completely absent in both study groups. The distribution of the polymorphic genotypes among the study groups was not significantly different (p = 0. 0.398). The frequency of the D allele was 0.76 in the patients and 0.77 in the control group, while the frequency of I allele was 0.24 in the patients and 0.23 in the control group. No deviation from Hardy–Weinberg equilibrium was observed (χ²= 4.24, df = 1, p = 0.12).

CONCLUSION: ACE I/D polymorphism is not associated with susceptibility to ALL among the Sudanese population.

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