The Effect of Strobilanthes crispus on Blood Glucose Levels and Lipid Profile of Streptozotocin-induced Diabetic Rats

Authors

  • Laras Ayu Fauziah Nur Fitri Department of Nutrition, Faculty of Health Sciences, Alma Ata University, Bantul, Yogyakarta, Indonesia
  • Tri Hernawati Department of Nutrition, Faculty of Health Sciences, Alma Ata University, Bantul, Yogyakarta, Indonesia
  • D. Desta Sari Department of Nutrition, Faculty of Health Sciences, Alma Ata University, Bantul, Yogyakarta, Indonesia
  • W. Indra Kusuma Department of Nutrition, Faculty of Health Sciences, Alma Ata University, Bantul, Yogyakarta, Indonesia
  • S. Kunti Department of Nutrition, Faculty of Health Sciences, Alma Ata University, Bantul, Yogyakarta, Indonesia
  • Hamam Hadi Graduate School of Public Health, Universitas Alma Ata, Bantul, Yogyakarta, Indonesia
  • Yulinda Kurniasari Department of Nutrition, Faculty of Health Sciences, Alma Ata University, Bantul, Yogyakarta, Indonesia
  • Effatul Afifah Department of Nutrition, Faculty of Health Sciences, Alma Ata University, Bantul, Yogyakarta, Indonesia https://orcid.org/0000-0002-1775-7822
  • Veriani Aprilia Department of Nutrition, Faculty of Health Sciences, Alma Ata University, Bantul, Yogyakarta, Indonesia

DOI:

https://doi.org/10.3889/oamjms.2022.9468

Keywords:

Diabetes mellitus, Strobilanthes crispus, Blood sugar, Lipid profile, Rat

Abstract

BACKGROUND: Diabetes mellitus is a metabolic disease associated with unhealthy eating patterns, a lack of physical activity, obesity, smoking habits, and family history. Strobilanthes crispus (SC) contains antioxidant compounds known to exert a hypoglycemic effect during long-term interventions in non-diabetic samples.

AIM: This study sought to determine the effect of administering the extract of SC leaves on the blood glucose levels and blood serum lipid profiles of streptozotocin (STZ)-induced diabetic rats.

METHODS: A total of 30 male Rattus norvegicus rats were divided into control and treatment groups. The control groups consisted of negative (normal rats) and positive controls (diabetic rats), whereas the treatment groups consisted of diabetic rats administered with 3.2% and 16.8% SC leaf extract, and glibenclamide as the drug comparison groups. Blood samples were collected from the rats’ retro-orbital vein for the purposes of glucose level and lipid profile measurement before and after the induction of diabetes with STZ as well as 14 days after the intervention. The collected data were analyzed statistically by means of a one-way analysis of variance continued with Duncan’s multiple range test.

RESULTS: The results showed the rats to be hyperglycemic experienced changes in their lipid profiles following the induction of diabetes. The administration of 3.2% SC leaf extract for 14 days reduced the rats’ blood glucose levels, while the effect of the 16.8% SC leaf extract was more pronounced in terms of reducing the rats’ blood glucose levels and improving their lipid profiles (reducing the triglyceride, total cholesterol, and low-density lipoprotein cholesterol levels, while increasing the high-density lipoprotein cholesterol levels).

CONCLUSIONS: This study found 16.8% SC leaf extract to exert a good hypoglycemic effect and leads to an improvement in the lipid profiles in diabetic rats.

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References

Dewi PS, Anisa IN. Effect of ethanolic extract of red betel on glucose level of hypercholestrolemic male Wistar rat. Kartika J Ilm Farm. 2014;2(2):7-13.

Yahya J, Stephanie, Wijaya E. Utilization of Keji Beling (Strobilanthes crispus BL) Tea as Alternative Medicine of Kidney Stones and Diabetes. Bogor: Institut Pertanian Bogor; 2009.

Aprilia V, Kurniasari Y, Hadi H, Nurinda E, Ariftiyana S. Effect of Porang Flour (Amorphophallus oncophyllus) on Blood Glucoese, Triglyceride, HDL, LDL, and Cholesterol of Streptozotocin-induced Diabetic; 2017.

Afifah E. Addition of sapodilla fruit (Manilkara zapota L.) decreased the blood glucose levels of diabetic rat (Rattus norvegicus). Indonesian J Nutr Diet. 2016;3(3):180.

Francisco BM, Salvador M, Amparo N. Oxidative stress in myopia. Oxid Med Cell Longev. 2015;2015:750637. https://doi.org/10.1155/2015/750637 PMid:25922643

Purwaningtyastuti R, Nurwanti E, Huda N. Vitamin C consumption related with blood glucose levels of Type 2-diabetes outpatients. Indonesian J Nutr Diet. 2018;5(1):44.

Silalahi M. Utilization of Strobilanthes crispa as traditional medicine and its bioactivity. J Edukasi Mat Sains. 2020;9:196-205.

Nurraihana H, Norfarizan-Hanoon NA. Phytochemistry, pharmacology and toxicology properties of Strobilanthes crispus. Int Food Res J. 2013;20(5):2045-56.

Norfarizan-Hanoon NA, Asmah R, Rokiah MY, Fauziah O, Faridah H. Absence of toxicity of Strobilanthes crispa juice in acute oral toxicity study in Sprague Dawley rats. Sains Malaysiana. 2012;41(4):403-9.

Fadzelly AB, Asmah R, Fauziah O. Effects of Strobilanthes crispus tea aqueous extracts on glucose and lipid profile in normal and streptozotocin-induced hyperglycemic rats. Plant Foods Hum Nutr. 2006;61(1):7-12. https://doi.org/10.1007/s11130-006-0002-z PMid:16688478

Oktavia S, Eriadi A, Valdis S. Effect of ethanolic extract of kejibeling (Strobilanthes crispa Blume) leave on cholesterol levels and LDL of hypocholesterolemic male white rats. J Farm Higea. 2018;10(2):110-5.

Lim KT, Lim V, Chin JH. Subacute oral toxicity study of ethanolic leaves extracts of Strobilanthes crispus in rats. Asian Pac J Trop Biomed. 2012;2(12):948-52. https://doi.org/10.1016/S2221-1691(13)60005-2 PMid:23593574

Reeves PG, Neilsen FH, Fahey, GC Jr. AIN-93 purified diets for laboratory rodents: Final report of the American institute of nutrition Ad Hoc writing committee on the formulation of the AIN-76A rodent diet. J Nutr. 1993;123(11):1939-51. https://doi.org/10.1093/jn/123.11.1939 PMid:8229312

Ghasemi A, Khalifi S, Jedi S. Streptozotocin-nicotinamide-induced rat model of Type 2 diabetes (review). Acta Physiol Hung. 2014;101(4):408-20. https://doi.org/10.1556/APhysiol.101.2014.4.2 PMid:25532953

Rias YR, Sutikno E. Relationship between body weight and random blood glucose levels of diabetic rats. J Wiyata. 2017;4(1):72-7.

Association AD. Classification and diagnosis of diabetes melitus. Diabetes Care. 2015;38 Suppl: S8-16. https://doi.org/10.2337/dc15-S005 PMid:25537714

Nugroho AE. Animal models of diabetes mellitus: Pathology and mechanism of some diabetogenics. Biodivers J Biol Divers. 2006;7(4):378-82.

Jaiswal YS, Tatke PA, Gabhe SY, Vaidya AB. Antidiabetic activity of extracts of Anacardium occidentale Linn. leaves on n-streptozotocin diabetic rats. J Tradit Complement Med. 2016;7(4):421-7. https://doi.org/10.1016/j.jtcme.2016.11.007 PMid:29034189

Snehalata C, Ramachandran A. Diabetes Mellitus in Public Nutrition and Health. Jakarta, Indonesia: Penerbit Buku Kedokteran EGC; 2009.

Nonci FY, Leboe DW, Armalia. Activity of ethanolic extract of keji beling (Strobilanthes crispus Linn) in decreasing of blood glucose levels of male rats (Mus musculus). JF FIK UINAM. 2016;4(1):16-20.

Ismawati I, Sembirin LP. The effect of alpha lipoic acid in preventing atherosclerosis lesion on the abdominal aorta of diabetes mellitus Type 2 rats. J Kedokt dan Kesehat. 2017;5(3):155-160.

Ashari A, Nurinda E, Fatmawati A. Increase of insulin levels of Streptozotocin-induced male Wistar rats (Rattus norvegicus) after ethanolic extract treatment of brotowali stem (Tinospora crispa L.). Indonesian Pharm Nat Med J. 2021;5(1):1.

Widowati W. Potency of antioxidant as antidiabetes. J Kesehat Masy. 2008;7(2):193-202.

Suryaatmadja M. Triglyceride test without fasting. Summit Lipid Update. 2012;7:1-2.

Nugraheni M, Lastariwati B, Purwanti S. Proximate and chemical analysis of gluten-free enriched, resistant starch Type 3 from Maranta arundinacea flour and its potential as a functional food. Pak J Nutr. 2017;16(5):322-30.

Pradana DA, Dwiratna DW, Widyarini S. Activity of standardized-ethanolic extract of red spinach (Amaranthus tricolor L.) as preventive efforts of steatosis: In vivo study. J Sains Farm Klin. 2017;3(2):120.

Nurhidajah, Astuti R, Nurrahman. Black rice potential in HDL and LDL profile in Sprague Dawley rat with high cholesterol diet. IOP Conf Ser Earth Environ Sci. 2019;292(1):012019.

Trivedi NA, Mazumdar B, Bhatt JD, Hemavathi KG. Effect of shilajit on blood glucose and lipid profile in alloxan- induced diabetic rats. Indian J Pharmacol. 2004;36(6):373-6.

Yassin MM, Mwafy SN. Protective potential of glimepiride and Nerium oleander extract on lipid profile, body growth rate, and renal function in streptozotocin-induced diabetic rats. Turkish J Biol. 2007;31(2):95-102.

Adam J. Dislipidemia: Internal Medicine Science. 4th ed. Jakarta, Indonesia: Pusat Department of Internal Medicine Science, Medicine Faculty. Universitas Indonesia; 2006.

Riyanto S, Muwarni RH. Black soyghurt lowered LDL levels of hypercholesterolemic rats. Indonesian J Nutr Diet. 2016;3(1):1.

Potter SM. Overview of Proposed Mechanisms for the hypocholesterolemic effects os soy. J Nutr. 1995;125:606.

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Published

2022-01-03

How to Cite

1.
Fitri LAFN, Hernawati T, Sari DD, Kusuma WI, Kunti S, Hadi H, Kurniasari Y, Afifah E, Aprilia V. The Effect of Strobilanthes crispus on Blood Glucose Levels and Lipid Profile of Streptozotocin-induced Diabetic Rats. Open Access Maced J Med Sci [Internet]. 2022 Jan. 3 [cited 2022 Aug. 13];10(T8):35-40. Available from: https://oamjms.eu/index.php/mjms/article/view/9468

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