Formulation, Evaluation of Physical Properties, and In Vitro Antioxidant Activity Test of Moringa Leaf (Moringa oleifera L.) Ethanolic Extract Capsules

Annisa Fatmawati; Depita Sucianingsih; Revalina Riswan; Emelda Emelda; Nurul Kusumawardhani; Rizal Fauzi; Daru Estiningsih; Muhammad Abdurrahman Munir; Marisa Yansiani; Hamam Hadi; Mika Matsuzaki

doi:10.3889/oamjms.2022.9499

Authors

Annisa Fatmawati Department of Pharmacy, Faculty of Health Science, Alma Ata University, Yogyakarta, Indonesia https://orcid.org/0000-0002-8677-0932
Depita Sucianingsih Department of Pharmacy, Faculty of Health Science, Alma Ata University, Yogyakarta, Indonesia
Revalina Riswan Department of Pharmacy, Faculty of Health Science, Alma Ata University, Yogyakarta, Indonesia
Emelda Emelda Department of Pharmacy, Faculty of Health Science, Alma Ata University, Yogyakarta, Indonesia https://orcid.org/0000-0002-6380-3869
Nurul Kusumawardhani Department of Pharmacy, Faculty of Health Science, Alma Ata University, Yogyakarta, Indonesia https://orcid.org/0000-0003-3554-9039
Rizal Fauzi Department of Pharmacy, Faculty of Health Science, Alma Ata University, Yogyakarta, Indonesia
Daru Estiningsih Department of Pharmacy, Faculty of Health Science, Alma Ata University, Yogyakarta, Indonesia
Muhammad Abdurrahman Munir Department of Pharmacy, Faculty of Health Science, Alma Ata University, Yogyakarta, Indonesia https://orcid.org/0000-0001-6129-6202
Marisa Yansiani Department of Pharmacy, Faculty of Health Science, Alma Ata University, Yogyakarta, Indonesia
Hamam Hadi Department of Public Health, Graduate School, Faculty of Health Sciences, Alma Ata University, Yogyakarta, Indonesia https://orcid.org/0000-0002-1945-2539
Mika Matsuzaki Department of Human Nutrition, International Health, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland, USA https://orcid.org/0000-0002-7020-3757

DOI:

https://doi.org/10.3889/oamjms.2022.9499

Keywords:

Antioxidant, Moringa oleifera L, Capsule, COVID-19

Abstract

BACKGROUND: Supplements that contain antioxidants may enhance prevention and treatment effects of a wide range of diseases including COVID-19. Quercetin, a flavonoid compound, is a natural antioxidant that can neutralize free radicals. The current study was conducted to formulate Moringa leaf (Moringa oleifera L) ethanol extract capsules and to determine the quercetin antioxidant activity levels of Moringa ethanol extract capsule formulations.

MATERIALS AND METHODS: We tested the total flavonoid levels in solutions with concentrations of 20, 50, 60, 70 and 100 ppm using Thin Layer Chromatography Densitometric method. Evaluation of physical properties of 96% Moringa leaf ethanol extract capsules included moisture content test, granule angle of repose test, granule flow property test, capsule weight uniformity test and capsule disintegration time test. Antioxidant activity test using the DPPH method with two samples, namely 96% Moringa leaf ethanol extract capsules with formulas I, II, III, quercetin as a comparison.

RESULT: The results of the evaluation of 96% Moringa leaf ethanol extract capsules showed that formula II (PVP 50 mg) had good physical properties. Testing the antioxidant activity of capsules of ethanol extract of Moringa leaves formulas I, II, III, quercetin obtained IC50 values of 44.0 ppm, 40.2 ppm, 46.4 ppm, and 4.80 ppm, respectively.

CONCLUSION: The evaluation of the ethanol extract capsules of Moringa leaf formula II, met the parameters of a good capsule evaluation test requirement and had very strong antioxidant activity seen from the acquisition of the IC50 value. The antioxidant properties of Moringa leaf extract capsules may be able to improve the immune system and clinical trials need to be carried out on patients to become candidates for prevention and therapeutic supplement fora range of diseases including COVID-19.

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