Essential Oil Constituents and Pharmacognostic Evaluation of Java Citronella (Cymbopogon winterianus) stem from Bandung, West Java, Indonesia

Ayu Nala El Muna Haerussana; Haura Fatona Chairunnisa

doi:10.3889/oamjms.2022.9546

Authors

Ayu Nala El Muna Haerussana Department of Pharmacy, Poltekkes Kemenkes Bandung, Bandung, Indonesia; Center of Excellence on Utilization of Local Material for Health Improvement, Poltekkes Kemenkes Bandung, Bandung, Indonesia
Haura Fatona Chairunnisa Department of Pharmacy, Poltekkes Kemenkes Bandung, Bandung, Indonesia

DOI:

https://doi.org/10.3889/oamjms.2022.9546

Keywords:

Cymbopogon winterianus stem, Java citronella, Gas chromathography-mass spectroscopy, Physicochemical, Essential oil

Abstract

BACKGROUND: Cymbopogon winterianus essential oil contains citral-derived chemicals with a variety of pharmacological effects, although there has been minimal research on pharmacognostic, phytochemical, and biological aspects.

AIM: This research aims to evaluate the pharmacognostic and chemical components of C. winterianus stem essential oil.

METHODS: The pharmacognostic studies were carried out in terms of macroscopic, microscopic, water content, water extractive values, ethanol extractive values, and essential oil yield. The oil was extracted by ethanol steam distillation method, the oil composition was analyzed by GC-MS.

RESULTS: Cymbopogon winterianus stems are 15-35 cm long and 0.5-2 cm broad, with a rough, stiff, and thin texture, with prominent fibers on the top and bottom surfaces. It has a bitter and slightly spicy taste with a distinctive lemony aroma and was greenish yellow in hue with a blend of purplish-red colors. The upper and lower epidermis was examined under a microscope, which revealed sclerenchyma fibers, trichomes, parenchyma, calcium oxalate (rosette), cortex, stem pith, oil cells, stomata (Gramineae type), trachea (ladder and spiral thickening), and collenchyma. The water content was 7.16±0.72%, the water-soluble extractive value was 12.152±0.003%, the ethanol-soluble extractive value was 12.290 ±0.76%, and the essential oil content was 6.46±0.50%. Essential oil constituents were 6-octen-1-ol, 3,7-dimethyl-, formate; trifluoroacetyl-lavandulol; n-hexadecanoic acid; 9,12-octadecadienoic acid (Z,Z)-; oxacyclotetradecane-2,11-dione, 13-methyl-; palmitic acid vinyl ester; glycidyl palmitate; 9,17-Octadecadienal, (Z)-; glycidyl palmitoleate; 1,4-bis(trimethylsilyl)benzene; and cyclotrisiloxane, hexamethyl-.

CONCLUSION: The essential oil constituents and preliminary pharmacognostic evaluation of Cymbopogon winterianus stem can provide useful data for further phytochemical analysis, quality control, and standardization of Cymbopogon winterianus. Citronellyl formate detected as citral derivatives as the main compound in the ethanol steam distillation method.

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References

Cahyaningsih R, Brehm JM, Maxted N. Gap analysis of Indonesian priority medicinal plant species as part of their conservation planning. Glob Ecol Conserv. 2021;26:e01459. https://doi.org/10.1016/j.gecco.2021.e01459 DOI: https://doi.org/10.1016/j.gecco.2021.e01459

Von Rintelen K, Arida E, Häuser C. A Review of biodiversity-related issues and challenges in megadiverse Indonesia and other Southeast Asian Countries. Res Ideas Outcomes. 2017;3:e20860. https://doi.org/10.3897/rio.3.e20860. DOI: https://doi.org/10.3897/rio.3.e20860

Ministry of Trade of The Republic of Indonesia. Handbook of Commodity Profile Indonesian Herbal: The Traditional Therapy. Indonesia: TREDA Ministry of Trade, Republic of Indonesia; 2009. https://doi.org/10.12695/ajtm.2015.8.2.2 DOI: https://doi.org/10.12695/ajtm.2015.8.2.2

Dacosta M, Sudirga SK, Muksin IK. Comparison plant contains oil of citronella (Cymbopogon nardus Rendle L.) grown in different locations. J Simbiosis. 2017;5(1):25-31. https://doi.org/10.24843/jsimbiosis.2017.v05.i01.p06 DOI: https://doi.org/10.24843/JSIMBIOSIS.2017.v05.i01.p06

Verma RS, Verma SK, Tandon S, Padalia RC, Darokar MP. Chemical composition and antimicrobial activity of Java citronella (Cymbopogon winterianus Jowitt ex Bor) essential oil extracted by different methods. J Essent Oil Res. 2020;32(5):449-55. https://doi.org/10.1080/10412905.2020.1787885 DOI: https://doi.org/10.1080/10412905.2020.1787885

Avoseh O, Oyedeji O, Rungqu P, Nkeh-Chungag B, Oyedeji A. Cymbopogon species; ethnopharmacology, phytochemistry and the pharmacological importance. Molecules. 2015;20(5):7438-53. https://doi.org/10.3390/molecules20057438 PMid:25915460 DOI: https://doi.org/10.3390/molecules20057438

Mariam T, Oktiviyari A, Harahap AY. The effect of lemongrass leaves and stalks extracts using methanol as the eco-friendly larvicides on fourth instar Aedes aegypti larvae. Open Access Maced J Med Sci. 2021;9(B):937-9. https://doi.org/10.3889/oamjms.2021.6727 DOI: https://doi.org/10.3889/oamjms.2021.6727

Maulid D, Bahar B, Sirajuddin S, Hadju V, Citrakesumasari C, Masni M. Effect of the stems lemongrass (Cymbopogon citratus) in pallumara and pepes anchovy (Stolephorus sp.) to uric acid levels of hyperuricemia elderly women. Open Access Maced J Med Sci. 2020;8(T2):109-14. https://doi.org/10.3889/oamjms.2020.5203 DOI: https://doi.org/10.3889/oamjms.2020.5203

Wany A, Kumar A, Nallapeta S, Jha S, Nigam VK, Pandey DM. Extraction and characterization of essential oil components based on geraniol and citronellol from Java citronella (Cymbopogon winterianus Jowitt). Plant Growth Regul. 2014;73(2):133-45. https://doi.org/10.1007/s10725-013-9875-7 DOI: https://doi.org/10.1007/s10725-013-9875-7

Katiyar R, Gupta S, Yadav KR. Cymbopogon winterianus : An important species for essential Java citronella oil and medicinal value. In: National Conference on Forest Biodiversity : Earth’s Living Treasure. Lucknow: Uttar Pradesh Biodiversity Board; 2011. p. 115-8.

De Oliveira WA, Pereira FO, De Luna GC, Lima IO, Wanderley PA, De Lima PA, et al. Antifungal activity of Cymbopogon winterianus Jowitt Ex Bor against Candida albicans. Braz J Microbiol. 2011;42(2):433-41. https://doi.org/10.1590/S1517-83822011000200004 PMid:24031651 DOI: https://doi.org/10.1590/S1517-83822011000200004

Leite BL, Souza TT, Antoniolli AR, Guimarães AG, Siqueira RS, Quintans JS, et al. Volatile constituents and behavioral change induced by Cymbopogon winterianus leaf essential oil in rodents. Afr J Biotechnol. 2011;10(42):8312-9. https://doi.org/10.1016/j.phymed.2007.09.018 DOI: https://doi.org/10.1016/j.phymed.2007.09.018

Andila PS, Hendra IPA, Wardani PK, Tirta IG, Fardenan D. The phytochemistry of Cymbopogon winterianus essential oil from Lombok Island, Indonesia and its antifungal activity against phytopathogenic fungi. Nusant Biosci. 2018;10(4):232-9. https://doi.org/10.13057/nusbiosci/n100406 DOI: https://doi.org/10.13057/nusbiosci/n100406

Da Costa AS, Hott MC, Horn AH. Management of citronella (Cymbopogon winterianus Jowitt ex Bor) for the production of essential oils. SN Appl Sci. 2020;2(12):2132. https://doi.org/10.1007/s42452-020-03949-8 DOI: https://doi.org/10.1007/s42452-020-03949-8

Kusumaningrum HP, Zainuri M, Endrawati H, Purbajanti ED. Characterization of Citronella Grass Essential Oil of Cymbopogon Winterianus from Batang Region, Indonesia. In: Journal of Physics: Conference Series 1524 012057. Bristol: Institute of Physics Publishing; 2020. https://doi.org/10.1088/1742-6596/1524/1/012057 DOI: https://doi.org/10.1088/1742-6596/1524/1/012057

Alexander SK, Strete D, Niles MJ. Laboratory Exercises in Organismal and Molecular Microbiology. New York: The McGraw Hill Companies; 2003.

Ministry of Health of RI. Indonesian Herbal Pharmacopeia. 2nd ed. Jakarta: Ministry of Health RI; 2017.

Ministry of Health RI. Indonesian Materia Medica. Vol. 5. Jakarta: Ministry of Health RI; 1989.

Ma ZY, Wen J, Ickert-Bond SM, Chen LQ, Liu XQ. Morphology, structure, and ontogeny of trichomes of the grape genus (Vitis, vitaceae). Front Plant Sci. 2016;7:704. https://doi.org/10.3389/fpls.2016.00704 PMid:27252720 DOI: https://doi.org/10.3389/fpls.2016.00704

Nunes TD, Zhang D, Raissig MT. Form, development and function of grass stomata. Plant J. 2020;101(4):780-99. https://doi.org/10.1111/tpj.14552 PMid:31571301 DOI: https://doi.org/10.1111/tpj.14552

Chandel HS, Pathak AK, Tailang M. Standardization of some herbal antidiabetic drugs in polyherbal formulation. Pharmacognosy Res. 2011;3(1):49-56. https://doi.org/10.4103/0974-8490.79116 PMid:21731396 DOI: https://doi.org/10.4103/0974-8490.79116

Kiromah NZ, Septiani SW, Rahmatulloh W, Aji PA. Establishment of standard parameters of crude drugs and ethanolic extract of ceylon olive (Elaeocarpus serratus L.) leaves. Pharm J Indones. 2020;17(01):207-15. https://doi.org/10.30595/pharmacy.v17i1.8833 DOI: https://doi.org/10.30595/pharmacy.v17i1.8833

Sankeshwari R, Ankola A, Bhat K, Hullatti K. Soxhlet versus cold maceration: Which method gives better antimicrobial activity to licorice extract against Streptococcus mutans? J Sci Soc. 2018;45(2):67-71. https://doi.org/10.4103/jss.jss_27_18 DOI: https://doi.org/10.4103/jss.JSS_27_18

Momin RK, Kadam VB. Determination of soluble extractive of some medicinal plants of genus Sesbania of Marathwada region in Maharashtra. Int J Life Sci Pharma Res. 2012;2(2):L-1-L-4.

Zhang QW, Lin LG, Ye WC. Techniques for extraction and isolation of natural products: A comprehensive review. Chinese Med. 2018;13(1):20. DOI: https://doi.org/10.1186/s13020-018-0177-x

Purba OH, Tumanggor NT, Syafitri A, Meliala L, Masauli D, Fakultas S, et al. Preparation of balsam sticks from lemongrass (Cymbopogon citratus (DC.) Stapf) as aromatherapy. [Pembuatan sediaan balsem stick dari sereh (Cymbopogon citratus (DC.) Stapf) sebagai aromaterapi]. J Penelit Farm Herb. 2021;3(1):75-81. https://doi.org/10.36656/jpfh.v3i1.326 DOI: https://doi.org/10.36656/jpfh.v3i1.326

Adama KK, Edoga MO. Comparative study of production oil from avocado apple (Persea americana) using steam distillation and extraction. Arch Appl Sci Res. 2011;3(4):411-23.

Fitri N, Riza R, Akbari MK, Khonitah N, Fahmi RL, Fatimah I. Identification of citronella oil fractions as efficient bio-additive for diesel engine fuel. Designs. 2022;6(1):15. https://doi.org/10.3390/designs6010015 DOI: https://doi.org/10.3390/designs6010015

Hamzah MH, Man HC, Abidin ZZ, Jamaludin H. Comparison of citronella oil extraction methods from Cymbopogon nardus grass by ohmic-heated hydro-distillation, hydro-distillation, and steam distillation. BioResources. 2014;9(1):256-72. https://doi.org/10.15376/biores.9.1.256-272 DOI: https://doi.org/10.15376/biores.9.1.256-272

Džamić AM, Soković MD, Ristić MS, Grujić SM, Mileski KS, Marin PD. Chemical composition, antifungal and antioxidant activity of Pelargonium graveolens essential oil. J Appl Pharm Sci. 2014;4(3):1-5. https://doi.org/10.7324/japs.2014.40301 DOI: https://doi.org/10.7324/JAPS.2014.40301

Malik T, Singh P. Antimicrobial activity of aroma chemicals against uropathogens. J Environ Appl Bioresearch. 2015;3(2):86-91.

Taherpour AA, Maroofi H, Rafie Z, Larijani K. Chemical composition analysis of the essential oil of Melissa officinalis L. from Kurdistan, Iran by HS/SPME method and calculation of the biophysicochemical coefficients of the components. Nat Prod Res. 2012;26(2):152-60. https://doi.org/10.1080/14786419.2010.534733 PMid:21809949 DOI: https://doi.org/10.1080/14786419.2010.534733

Ungokore HY, Ehinmidu J, Onaolapo J, Olonitola OS. Assessment of antidermatophytic activity and chemical composition of Nigerian Citrus senensis (L.) Osbeck essential oil against multidrug-resistant pathogenic dermatophytes isolated from tinea capitis samples. Arch Pharm Sci Ain Shams Univ. 2021;5(2):275-87. https://doi.org/10.21608/aps.2021.87917.1066 DOI: https://doi.org/10.21608/aps.2021.87917.1066

Gouda B, Mousa O, Salama M, Kassem H. Volatiles and lipoidal composition: Antimicrobial activity of flowering aerial parts of Lavandula pubescens decne. Int J Pharmacogn Phytochem Res. 2017;9(8):1175-81. https://doi.org/10.25258/phyto.v9i08.9628 DOI: https://doi.org/10.25258/phyto.v9i08.9628

Mkolo NM, Gumede BT, Magano SR, Olaokun OO. Acaricidal and repellence of r. appendiculatus, and GC-MS chemical content of essential oils from three south african ethno-veterinary plants. Asian J Chem. 2021;33(6):1370-8. https://doi.org/10.14233/ajchem.2021.22995 DOI: https://doi.org/10.14233/ajchem.2021.22995

Dhanasezhian A, Srivani S, Rameshkumar MR. Nitric oxide production and antioxidant activity of dried fruit extracts of Terminalia chebula. Asian J Pharm Clin Res. 2018;11(5):370-6. https://doi.org/10.22159/ajpcr.2018.v11i5.24316 DOI: https://doi.org/10.22159/ajpcr.2018.v11i5.24316

Ghalib RM, Hashim R, Sulaiman O, Awalludin MFB, Mehdi SH, Kawamura F. Fingerprint chemotaxonomic GC-TOFMS profile of wood and bark of mangrove tree Sonneratia caseolaris (L.) Engl. J Saudi Chem Soc. 2011;15(3):229-37. https://doi.org/10.1016/j.jscs.2010.09.003 DOI: https://doi.org/10.1016/j.jscs.2010.09.003

Moronkola DO, Faruq UZ, Adigun OA, Ajiboye CO. Essential oil compositions of leaf, stem-bark, stem, root, flower, and fruit with seed of Blighia unijugata Baker (Sapindaceae). African J Pharm Pharmacol. 2017;11(7):108-19. https://doi.org/10.5897/AJPP2016.4721 DOI: https://doi.org/10.5897/AJPP2016.4721

Momin K, Thomas SC. GC-MS analysis of antioxidant compounds present in different extracts of an endemic plant Dillenia scaberlla (Dilleniaceae) leaves and barks. Int J Pharm Sci Res. 2020;11(5):2262.

Lawrence AR, Paul JJ. Analysis of ethanolic extract of Pteridium aquilinum (L.) Kuhn: An important fern. J Drug Deliv Ther. 2019;9(4):285-7. https://doi.org/10.22270/jddt.v9i4.3044

Ayyakkannu P, Ganesh A, Packirisamy M, Ramalingam S, Venkataramanan S. Antioxidant potential of Eclipta alba, a traditional medicinal herb attenuates oxidative DNA damage in vitro. Nusant Biosci. 2020;12(1):73-8. https://doi.org/10.13057/nusbiosci/n120113 DOI: https://doi.org/10.13057/nusbiosci/n120113

Krishna SR, Hafza S, Chandrika PG, Priya CL, Rao KV. Pharmacological properties, phytochemical and GC-MS analysis of Bauhinia acuminata Linn. J Chem Pharm Res. 2015;7(4):372-80.

Surahmaida, Umarudin, Rani AW, Dewi NC. Phytochemical screening of secondary metabolite compounds methanol extract of Jatropha curcas leaf with GCMS. J Pharm Sci. 2021;6(1):25-30. https://doi.org/10.53342/pharmasci.v6i1.202 DOI: https://doi.org/10.53342/pharmasci.v6i1.202

Prakash A, Suneetha V. Punica granatum (pomegranate) rind extract as a potent substitute for L-ascorbic acid with respect to the antioxidant activity. Res J Pharm Biol Chem Sci. 2014;5(2):597-603.

Ismail GA, Gheda SF, Abo-Shady AM, Abdel-Karim OH. In vitro potential activity of some seaweeds as antioxidants and inhibitors of diabetic enzymes. Food Sci Technol. 2020;40(3):681-91. https://doi.org/10.1590/fst.15619 DOI: https://doi.org/10.1590/fst.15619

Guerrero RV, Abarca-Vargas R, Petricevich VL. Chemical compounds and biological activity of an extract from Bougainvillea X buttiana (var. Rose) Holttum and Standl. Int J Pharm Pharm Sci. 2017;9(3):42. https://doi.org/10.22159/ijpps.2017v9i3.16190 DOI: https://doi.org/10.22159/ijpps.2017v9i3.16190

Siswadi S, Saragih GS. Phytochemical Analysis of Bioactive Compounds in Ethanolic Extract of Sterculia Quadrifida R.Br. In: AIP Conference Proceedings 2353, 030098. Maryland: American Institute of Physics Inc.; 2021. https://doi.org/10.1063/5.0053057 DOI: https://doi.org/10.1063/5.0053057

Ragunath C, Kumar YA, Kanivalan I, Radhakrishnan S. Phytochemical screening and gc-ms analysis of bioactive constituents in the methanolic extract of Caulerpa racemosa (Forssk.) J. Agardh and Padina boergesenii Allender & Kraft. Curr Appl Sci Technol. 2020;20(3):380-93.

Aparna V, Dileep KV, Mandal PK, Karthe P, Sadasivan C, Haridas M. Anti-inflammatory property of n-hexadecanoic acid: Structural evidence and kinetic assessment. Chem Biol Drug Des. 2012;80(3):434-9. https://doi.org/10.1111/j.1747-0285.2012.01418.x PMid:22642495 DOI: https://doi.org/10.1111/j.1747-0285.2012.01418.x

Abubakar MN, Majinda RR. GC-MS analysis and preliminary antimicrobial activity of Albizia adianthifolia (Schumach) and Pterocarpus angolensis (DC). Medicines (Basel). 2016;3(1):3. https://doi.org/10.3390/medicines3010003 PMid:28930113 DOI: https://doi.org/10.3390/medicines3010003

Uma B, Prabhakar K, Rajendran S, Sarayu YL. Studies on GC/MS spectroscopic analysis of some bioactive antimicrobial compounds from Cinnamomum zeylanicum. J Med Plans. 2009;8(31):2022-5.

Ramya KS, Kanimathi P, Radha A. GC-MS analysis and antimicrobial activity of various solvent extracts from Simarouba glauca leaves. J Pharmacogn Phytochem. 2019;8(2):166-71.

Yakubu OE, Otitoju O, Onwuka J. Gas chromatography-mass spectrometry (GC-MS) analysis of aqueous extract of Daniellia oliveri stem bark. Pharm Anal Acta. 2017;8:11. https://doi.org/10.4172/2153-2435.1000568 DOI: https://doi.org/10.4172/2153-2435.1000568

Khanday S, Sharma GD. GC-MS analysis and antifeedant activity of Azaridiachta indica-leaf extract. Stechnolock Plant Biol Res. 2021;1:1-15.

Marrs WR, Horne EA, Ortega-Gutierrez S, Cisneros JA, Xu C, Lin YH, et al. Dual inhibition of α/β-hydrolase domain 6 and fatty acid amide hydrolase increases endocannabinoid levels in neurons. J Biol Chem. 2011;286(33):28723-8. https://doi.org/10.1074/jbc.M110.202853 PMid:21665953 DOI: https://doi.org/10.1074/jbc.M110.202853

Yuan J, Gan T, Liu Y, Gao H, Xu W, Zhang T, et al. Composition and antimicrobial activity of the essential oil from the branches of Jacaranda cuspidifolia Mart. growing in Sichuan, China. Nat Prod Res. 2018;32(12):1451-4. https://doi.org/10.1080/14786419.2017.1346644 PMid:28670931 DOI: https://doi.org/10.1080/14786419.2017.1346644

Krishnamoorthy K, Subramaniam P. Phytochemical profiling of leaf, stem, and tuber parts of Solena amplexicaulis (Lam.) Gandhi using GC-MS. Int Sch Res Not. 2014;2014:1-13. https://doi.org/10.1155/2014/567409 DOI: https://doi.org/10.1155/2014/567409

Christiana OA, Johnbull OE, Raphael CM, Joseph OO, Paul M, Emmanuel GJ. Gas Chromatographic Study of Bio-Active Compounds in Methanolic Extract of Leaf of Crateva Adansonii DC. In: Journal of Physics: Conference Series 1299 012014. Bristol: Institute of Physics Publishing; 2019. https://doi.org/10.1088/1742-6596/1299/1/012014 DOI: https://doi.org/10.1088/1742-6596/1299/1/012014