Assessment of Osteogenesis Enhancement in Rats Using Bone Densitometry: An In Vivo Study


  • Manar Abd Alrazaq Hassan Department of Anatomy, College of Medicine, University of Diyala, Baqubah, Iraq
  • Asmaa A. Ajwad Department of Physiology and Medical Physics, College of Medicine, University of Diyala, Baqubah, Iraq



Bone, BMD, BMC, Magnetized water, DXA


Background: Human survival depends on the availability of water. Safe drinking water is a fundamental human right as well as a basic need for a good health. At the mineral and organic levels of water composition, exposure to electromagnetic fields can cause considerable changes in the quality of drinking water. As a result, water is more easily absorbed into the cell walls throughout the body, making it better suited for organ growth and development. When drunk on a daily basis, magnetic water causes dramatic changes in bone mineral density and content. Due to the necessity for distinct beam filtering and near-perfect spatial discography of the two attenuations, DXA (DualEnergy X-ray Absorptiometry) was included with general-purpose X-ray systems. In rats that drink magnetized water on a daily basis, the DXA can be used to calculate the weight and area of all head compartments. Objective: By employing a Bone Densitometry scan on the head regions of rats, this study aims to assess the changes in the rat's skull bone measures (area, weight, bone mineral content, and bone mineral density) after feeding the animal with magnetized water. Methods: This study involved thirty adult male rats (6 weeks old). For 30 days, 15 rats were given a magnetized water to drink and used as experimental animals. For comparison purposes, the remaining 15 rats were given regular water for 30 days. After the experimental procedure was done, each rat's head was scanned with a DXA to determine bone weight, area, bone mineral content, and bone mineral density. Before drinking water, each rat in both the experimental and control groups was examined. The head of each rate was scanned using a DXA to get the values of bone: weight, area, mineral density, and mineral content after 30 days of consuming magnetized water. The four bone measures were statistically compared in the experimental and control rats, p-values below 0.05 were considered significant. Results: Comparing to control rats, experimental rats showed a significant increase in the bone’s weight and area of the skull (p<0.05, Wilcoxon test).  Bone marrow content and bone marrow density enhanced significantly as well in experimental rats after drinking the magnetized water for 30 days. Conclusions: Drinking water that has been subjected to electromagnetic fields for 30 days can improve bone marrow content and density in rats. Drinking magnetized water also has a substantial impact on bone mass and area.


Download data is not yet available.


Metrics Loading ...

Plum Analytics Artifact Widget Block


Alrazaq MA, Hassan A, Al-Ghaban NM. Immunohistochemical localization of bone morphogenic protein-2 in extracted tooth socket treated by local application of grape seeds oil in rabbits. Biochem Cell Arch. 2020;20(1):581-9.

Hassan MA. Determined the biological changes in the head of the rats consumed magnetically affected water by body scan devise. Indian J Forensic Med Toxicol. 2021;15(3):3596-603. DOI:

Prado CM, Heymsfield SB. Lean tissue imaging: A new era for nutritional assessment and intervention. JPEN J Parenter Enter Nutr. 2014;38(8):940-53. PMid:25239112 DOI:

Prado CM, Siervo M, Mire E, Stephan BC, Broyles S, Smith SR, et al., A population-based approach to define bodycomposition. Am J Clin Nutr. 2014;99(6):1369-77. PMid:24760978 DOI:

Kranioti EF, Bonicelli A, García-donas JG. Bone-mineral density : Clinical signi fi cance methods of quanti fi cation and forensic applications. Res Rep Forensic Med Sci. 2019;9:9-21. DOI:

Hassan NA, Mubarak TH, Gheaeb NF. Evaluation the effect of magnetized water on the bone density and osteoporosis in the experimental rats by dxa- scan. Diyala J Med. 2019;17(2):1-14. DOI:

Ikezoe Y, Hirota N, Nakagawa J, Kitazawa K. Making water levitate. Nature. 1998;393(6687):749-50. DOI:

Hussein AN, Sami AS, Al-Badry KI, Jabir AA, Faisel IF, Abd RB, Effect of magnetic water drinking on testis dimension, scrotal circumference and blood parameters of holstein bulls born in Iraq. Adv Anim Vet Sci. 2015;3(7):413-7. DOI:

Ebrahim SA, Azab AE. Biological effects of magnetic water on human and animals. Biomed Sci. 2017;3(4):78-85. DOI:

Yacout H, Hassan AA, Khalel MS, Shwerab AM, Abdel-Gawad EI, Yi AK. Effect of Magnetic Water on the Performance of Lactating Goats. J Dairy Vet Anim Res. 2015;2(5):159-70. DOI:

Yates AJ, Ross PD, Lydick E, Epstein RS. Radiographic absorptiometry in the diagnosis of osteoporosis. Am J Med. 1995;98(2):41S-7. PMid:7709934 DOI:

Genant HK, Engelke K, Fuerst T, Glüer CC, Grampp S, Harris ST, et al. Noninvasive assessment of bone mineral and structure: State of the art harry. J Bone Miner Res. 1996;11(6):707-30. PMid:8725168 DOI:

Heymsfield SB, Wang Z, Baumgartner RN, Ross R. Human body composition: Advances in models and methods. Annu Rev Nutr. 1997;17:527-58. PMid:9240939 DOI:

Heymsfield SB, Adamek M, Gonzalez MC, Jia G, Thomas DM. Assessing skeletal muscle mass: Historical overview and state of the art. J Cachexia Sarcopenia Muscle. 2014;5(1):9-18. PMid:24532493 DOI:

Heymsfield SB, Smith R, Aulet M, Bensen B, Lichtman S, Wang J, et al. Appendicular skeletal muscle mass: Measurement by dual-photon absorptiometry. Am J Clin Nutr. 1990;52(2):214-8. PMid:2375286 DOI:

Madsen OR, Egsmose C, Lorentzen JS, Lauridsen UB, Sørensen OH. Infuence of orthopaedic metal and high-density detection on body composition as assessed by dual-energy Xray absorptiometry. Clin Physiol Funct Imaging. 1999;19(3):238-45. PMid:10361614 DOI:

Heymsfield SB, Lohman TG, Wang ZM, Goin SB. Human Body Composition. 2nd ed. Champaign: Human Kinetics; 2005. DOI:

Genton L, Hans D, Kyle UG, Pichard C. Dual-energy X-ray absorptiometry and body composition: differences between devices and comparison with reference methods. Nutrition. 2002;8(1):66-70. PMid:11827768 DOI:

Petak S, Barbu CG, Yu EW, Fielding R, Mulligan K, Sabowitz B, et al. The official positions of the international society for clinical densitometry: Body composition analysis reporting. J Clin Densitom. 2013;16(4):508-19. PMid:24183640 DOI:

Hassan NA. The effect of magnetized nutrition on bone density A. Diyala J of Med. 2019;16(1):70-82. DOI:




How to Cite

Hassan MAA, Ajwad AA. Assessment of Osteogenesis Enhancement in Rats Using Bone Densitometry: An In Vivo Study. Open Access Maced J Med Sci [Internet]. 2022 Apr. 28 [cited 2023 Mar. 22];10(A):1268-72. Available from: