The Effectivity of IC50 Test between Green Tea and Curcumin Extracts from Mt. Lawu as an Antioxidant for SOD and MDA Levels in a Cisplatin Rat Model


  • Novi Primadewi Medical Science Doctoral Study Program, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Indonesia
  • Harijono Kariosentono Department of Dermatovenereology, Faculty of Medicine, Universitas Sebelas Maret, Dr. Moewardi General Hospital, Surakarta, Indonesia
  • Ari Probandari Department of Public Health, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Indonesia
  • Budiyanti Wiboworini Department of Nutrition Sciences, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Indonesia



Superoxide dismutase, Malondialdehyde, Ototoxicity, Antioxidant, IC50



Purpose: This study aims to determine the relationship of the effective dose between Mt. Lawu green tea and Mt. Lawu curcumin as antioxidants based on superoxide dismutase (SOD) and malondialdehyde (MDA) levels.

Methods: Blood was undertaken through the orbital sinus and allowed to stand until serum was formed. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) method was used to observe the effective dose of Mt. Lawu green tea and curcumin by quantifying the color change of each sample after incubation. The color change measured the absorbance value through a spectrophotometer. The data were analyzed using a correlation test to measure the effective concentration of Mt. Lawu green tea and Mt. Lawu curcumin toward SOD and MDA as antioxidant parameters in a cisplatin (CN) rat model.

Result: The study results show that the IC50 green tea concentration of 0.75 ± 0.16 µg/mL and the curcumin concentration of 5.3 ± 0.4 µg/mL can reduce 50% of DPPH. Based on the graphs, SOD increased and MDA decreased.

Discussion and Conclusion: This study demonstrates that the IC50 parameter of Mt. Lawu green tea and curcumin extracts is a good indicator for assigning the SOD and MDA levels in a CN rat model. Extracts derived from Mt. Lawu green tea and curcumin have ideal lowering DPPH, and the dosage has a strong relationship with the increase in SOD and the decrease in MDA.


Download data is not yet available.


Metrics Loading ...

Plum Analytics Artifact Widget Block


Dasari S, Tchounwou PB. Cisplatin in cancer therapy: Molecular mechanisms of action. Eur J Pharmacol. 2014;740:364-78. PMid:25058905 DOI:

Marullo R, Werner E, Degtyareva N, Moore B, Altavilla G, Ramalingam SS, et al. Cisplatin induces a mitochondrial- ROS response that contributes to cytotoxicity depending on mitochondrial redox status and bioenergetic functions. PLoS One. 2013;8(11):e81162. PMid:24260552 DOI:

Karasawa T, Steyger PS. An integrated view of cisplatin-induced nephrotoxicity and ototoxicity. Toxicol Lett. 2015;237(3):219-27. PMid:26101797 DOI:

Ding J, Wang H, Wu ZB, Zhao J, Zhang S, Li W. Protection of murine spermatogenesis against ionizing radiation-induced testicular injury by a green tea polyphenol. Biol Reprod. 2015;92(1):6. PMid:25395675 DOI:

Yan Z, Zhong Y, Duan Y, Chen Q, Li F. Antioxidant mechanism of tea polyphenols and its impact on health benefits. Anim Nutr. 2020;6(2):115-23. PMid:32542190 DOI:

Altobelli GG, Van Noorden S, Balato A, Cimini V. Copper/Zinc superoxide dismutase in human skin: Current knowledge. Front Med (Lausanne). 2020;7:183. PMid:32478084 DOI:

Setiowati L, Febrina L, Mahmudah F, Ramadhan AM, editors. Pengaruh Pemberian Infusa Daun Sirsak (Annona muricata L.) terhadap Profil Kadar Malondialdehida (MDA) Tikus Putih (Rattus norvegicus). Proceeding of Mulawarman Pharmaceuticals Conferences; 2018. DOI:

Munteanu IG, Apetrei C. Analytical methods used in determining antioxidant activity: A review. Int J Mol Sci. 2021;22(7):3380. PMid:33806141 DOI:

Shekhar TC, Anju G. Antioxidant activity by DPPH radical scavenging method of Ageratum conyzoides Linn. leaves. Am J Ethnomed. 2014;1(4):244-9.

Hussein YM, Mohamed RH, Shalaby SM, Abd El-Haleem MR, Abd El Motteleb DM. Anti-oxidative and anti-apoptotic roles of spermatogonial stem cells in reversing cisplatin-induced testicular toxicity. Cytotherapy. 2015;17(11):1646-54. PMid:26253898 DOI:

Banjarnahor SD, Artanti N. Antioxidant properties of flavonoids. Med J Indones. 2014;23(4):239-44. DOI:

Mao X, Gu C, Chen D, Yu B, He J. Oxidative stress-induced diseases and tea polyphenols. Oncotarget. 2017;8(46):81649-61. PMid:29113421 DOI:

Esen E, Özdoğan F, Gürgen SG, Özel HE, Başer S, Genç S, et al. Ginkgo biloba and lycopene are effective on cisplatin induced ototoxicity? J Int Adv Otol. 2018;14(1):22-6. PMid:28639555 DOI:

Sheth S, Mukherjea D, Rybak LP, Ramkumar V. Mechanisms of cisplatin-induced ototoxicity and otoprotection. Front Cell Neurosci. 2017;11:338. PMid:29163050 DOI:

Yiannakopoulou EC. Targeting oxidative stress response by green tea polyphenols: Clinical implications. Free Radic Res. 2013;47(9):667-71. PMid:23805775 DOI:

Alrawaiq NS, Abdullah A. A review of antioxidant polyphenol curcumin and its role in detoxification. Int J Pharm Tech Res. 2014;6(1):280-9.




How to Cite

Primadewi N, Kariosentono H, Probandari A, Wiboworini B. The Effectivity of IC50 Test between Green Tea and Curcumin Extracts from Mt. Lawu as an Antioxidant for SOD and MDA Levels in a Cisplatin Rat Model. Open Access Maced J Med Sci [Internet]. 2022 May 3 [cited 2023 Mar. 28];10(A):906-10. Available from:

Most read articles by the same author(s)