Relationship of Bactericidal Permeability Increasing Protein (BPI) Polymorphysm rs1341023, rs5743507, Tumor Necrosis Factor Alpha (TNF-α) rs361525, rs1800629 with Neonatal Sepsis

Afdal Afdal; Jamsari Jamsari; Yanwirasti Yanwirasti; Prambudi Rukmono

doi:10.3889/oamjms.2022.9762

Authors

Afdal Afdal Department of Biomedical, Faculty of Medicine, Andalas University, Padang, Indonesia
Jamsari Jamsari Department of Biomedical, Faculty of Medicine, Andalas University, Padang, Indonesia
Yanwirasti Yanwirasti Department of Biomedical, Faculty of Medicine, Andalas University, Padang, Indonesia
Prambudi Rukmono Department of Child Health, University of Lampung, Hospital of Abdul Moeluk, Lampung, Indonesia

DOI:

https://doi.org/10.3889/oamjms.2022.9762

Keywords:

Bactericidal permeability increasing protein, Tumor necrosis factor alpha, Neonatal, Polymorphism, Sepsis

Abstract

Background: Neonatal sepsis is a problem in the field of child health because the incidence is increasing every year and often ends in death. This study aimed to investigate the relationship between TNF-α and BPI gene polymorphisms with neonatal sepsis.

Methods: PCR and sequencing examinations were performed on 60 DNA samples consisting of 30 samples of neonatal sepsis and 30 samples of non-neoptic sepsis. Furthermore, data in the form of DNA mutation tables were statistically processed by univariate, bivariate, and multivariate analysis. It is considered meaningful if p<0.05.

Result: The results showed that BPI rs1341023, rs5743507 and TNF-α rs361525, rs1800629 only BPI rs1341023 and TNF-α rs1800629 were mutated. Of the two genes, only TNF-α rs1800629 had a significant association with neonatal sepsis (p<0.05).

Conclusion: This study proved that the TNF-α rs1800629 mutation was the most important polymorphism in the occurrence of neonatal sepsis (p<0.05).

Keywords: BPI, TNF-α, Neonatal, Polymorphism, Sepsis

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References

Fleischmann C, Scherag A, Adhikari NK, Hartog CS, Tsaganos T, Schlattmann P, et al. Assessment of Global Incidence and mortality of hospital-treated sepsis. Current estimates and limitations. Am J Respir Crit Care Med. 2016;193(3):259-72. https://doi.org/10.1164/rccm.201504-0781OC PMid:26414292 DOI: https://doi.org/10.1164/rccm.201504-0781OC

Kumar G, Kumar N, Taneja A, Kaleekal T, Tarima S, McGinley E, et al. Nationwide trends of severe sepsis in the 21st century (2000-2007). Chest. 2011;140(5):1223-31. https://doi.org/10.1378/chest.11-0352 PMid:21852297 DOI: https://doi.org/10.1378/chest.11-0352

World Health Organization. Sepsis. Tersedia Pada; 2018. Available from: https://www.who.int/news-room/fact-sheets/detail/sepsis [Last accessed on 2020 Jan 08].

Nupponen I, Turunen R, Nevalainen T, Peuravuori H, Pohjavuori M, Repo H, et al. Extracellular release of bactericidal/permeability-increasing protein in newborn infants. Pediatr Res. 2002;51(6):670-4. https://doi.org/10.1203/00006450-200206000-00002 PMid:12032259 DOI: https://doi.org/10.1203/00006450-200206000-00002

Rukmono P. Polimorfisme Asp2999Gly dan Thr399Ile Gen TLR-4 Pada Sepsis Neonatorum serta Hubungannya dengan Kadar TNF-α dan IL-6. Indonesian: Universitas Andalas; 2015.

Tumangger H, dan Jamil KF. Contribution of genes polymorphism to susceptibility and outcome of sepsis. Egypt J Med Hum Genet. 2010;11(2):97-103. https://doi.org/10.1016/j.ejmhg.2010.10.001 DOI: https://doi.org/10.1016/j.ejmhg.2010.10.001

Esposito S, Zampiero A, Pugni L, Tabano S, Pelucchi C, Ghirardi B, et al. Genetic polymorphisms and sepsis in premature neonates. PLoS One. 2014;9(7):e101248. https://doi.org/10.1371/journal.pone.0101248 10 PMid:25000179 DOI: https://doi.org/10.1371/journal.pone.0101248

Abu-Maziad A, Schaa K, Bell EF, Dagle JM, Cooper M, Marazita ML, et al. Role of polymorphic variants as genetic modulators of infection in neonatal sepsis. Pediatr Res. 2010;68(4):323-9. https://doi.org/10.1203/PDR.0b013e3181e6a068 PMid:20463618 DOI: https://doi.org/10.1203/PDR.0b013e3181e6a068

Flori L, Delahaye NF, Iraqi FA, Hernandez-Valladares M, Fumoux F, Rihet P. TNF as a malaria candidate gene: Polymorphism-screening and family-based association analysis of mild malaria attack and parasitemia in Burkina Faso. Genes Immun. 2005;6(6):472-80. https://doi.org/10.1038/sj.gene.6364231 Mid:15931230 DOI: https://doi.org/10.1038/sj.gene.6364231

Zhang Y, Cui X, Ning L, Wei D. The effects of tumor necrosis factor-α (TNF-α) rs1800629 and rs361525 polymorphisms on sepsis risk. Oncotarget. 2017;8(67):111456-69. https://doi.org/10.18632/oncotarget.22824 PMid:29340067 DOI: https://doi.org/10.18632/oncotarget.22824

Song Z, Song Y, Yin J, Shen Y, Yao C, Sun Z, et al. Genetic variation in the TNF gene is associated with susceptibility to severe sepsis, but not with mortality. PLoS One. 2012;7(9):e46113. https://doi.org/10.1371/journal.pone.0046113 PMid:23029405 DOI: https://doi.org/10.1371/journal.pone.0046113

Gupta DL, Nagar PK, Kamal VK, Bhoi S, Rao DN. Clinical relevance of single nucleotide polymorphisms within the 13 cytokine genes in North Indian trauma hemorrhagic shock patients. Scand J Trauma Resusc Emerg Med. 2015;23(1):96. https://doi.org/10.1186/s13049-015-0174-3 PMid:26561011 DOI: https://doi.org/10.1186/s13049-015-0174-3

Treszl A, Kocsis I, Szathmári M, Schuler A, Héninger E, Tulassay T, et al. Genetic variants of TNF-α, IL-1β, IL-4 receptor α-chain, IL-6 and IL-10 genes are not risk factors for sepsis in low-birth-weight infants. Biol Neonate. 2003;83(4):241-5. https://doi.org/10.1159/000069484 PMid:12743452 DOI: https://doi.org/10.1159/000069484