Omega-3 and Vitamin E Supplementation Effect on Reactive Oxygen Species and Placental Vascular Endothelial Growth Factor: Pre-eclampsia Model


  • Arni Amir Department of Biology, Medical Faculty, Universitas Andalas, Padang, Indonesia
  • Eryati Darwin Department of Histology, Medical Faculty, Universitas Andalas, Padang, Indonesia



Omega-3, Vitamin E, ROS, VEGF, Pre-eclampsia


BACKGROUND: Pre-eclampsia (PE) has been associated with oxidative stress and inflammation. Omega-3 fatty acids and vitamin E have beneficial function to maintain cell membrane, prevent oxidative stress, and inhibit the production of proinflammatory cytokines. The purpose of the study was to determine the effect of omega-3 and vitamin E supplement to Reactive Oxygen Species (ROS) and placental Vascular Endothelial Growth Factor (VEGF) on PE rats model.

AIM: This study aimed to investigate the effect of omega-3 and vitamin E supplementation on ROS and placental VEGF.

METHODS: This research has been carried out at animal house and Biomedical Laboratory of the Medical Faculty Andalas University. The design of this research was experimental study with post-test only control group design. Thirty pregnant rats were divided into five groups. Group K-without treatment, group K+ was given L-NAME, group P1 was given L-NAME + omega-3, group P2 was given L-NAME + vitamin E, and group P3 was given L-NAME + omega-3 + vitamin E. The L-NAME dose was 50 mg/kg/day. At the 19th day of gestation, the pregnancy was terminated and the blood serum was used for examination of ROS and placental tissue was collected for examination of VEGF using ELISA kit. The data were analyzed by one-way ANOVA and post hoc test Bonferoni.

RESULTS: The analysis results found that there were significant differences between omega-3 and vitamin E supplements on ROS levels and placental VEGF levels. Administration of omega-3 only or combination with vitamin E can effectively improve endothelial function by increasing the level of VEGF.

CONCLUSION: Omega-3 (DHA 120 mg, EPA 180 mg) and vitamin E (α-tocopherol 300 iu) supplement decreased ROS and increased placental VEGF on pre-eclampsia rats model.


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POGI. PNPK Preeclampsia Diagnostic and Treatment; 2016. p. 1-48.

Kemse NG, Kale AA, Joshi SR. Supplementation of maternal omega-3 fatty acids to pregnancy induced hypertension Wistar rats improves IL10 and VEGF levels. Prostaglandins Leukot Essent Fat Acids. 2016;104:25-32. PMid:26802939 DOI:

Mochan S, Dhingra MK, Gupta SK, Saxena S, Arora P, Yadav V, et al. Status of VEGF in preeclampsia and its effect on endoplasmic reticulum stress in placental trophoblast cells. Eur J Obstet Gynecol Reprod Biol X. 2019;4:100070. PMid:31517301 DOI:

Kemse NG, Kale AA, Joshi SR. A combined supplementation of omega-3 fatty acids and micronutrients (folic acid, Vitamin B12) reduces oxidative stress markers in a rat model of pregnancy induced hypertension. PLoS One. 2014;9(11):29-31. PMid:25405347 DOI:

Aouache R, Biquard L, Vaiman D, Miralles F. Oxidative stress in preeclampsia and placental diseases. Int J Mol Sci. 2018;19(5):1496. DOI:

Matsubara K, Higaki T, Matsubara Y, Nawa A. Nitric oxide and reactive oxygen species in the pathogenesis of preeclampsia. Int J Mol Sci. 2015;16(3):4600-14. PMid:25739077 DOI:

Sugiritama IW, Ratnayanti IG, Wiryawan IG, Wahyuniari IA, Linawati NM. Effect of red fruit oil (Pandanus conoideus Lam) on animal model of preeclampsia. Int J Sci Res. 2016;5(7):1770-3. DOI:

Saghiri MA, Asatourian A, Ershadifar S, Moghadam MM, Sheibani N. Vitamins and regulation of angiogenesis: [A, B1, B2, B3, B6, B9, B12, C, D, E, K]. J Funct Foods. 2017;38:180-96. DOI:

Jones ML, Mark PJ, Waddell BJ. Maternal dietary omega-3 fatty acids and placental function. Reproduction. 2014;147(5):R143-52. PMid:24451224 DOI:

Zhuang W, Wang G, Li L, Lin G, Deng Z. Omega-3 polyunsaturated fatty acids reduce vascular endothelial growth factor production and suppress endothelial wound repair. J Cardiovasc Transl Res. 2013;6(2):287-93. PMid:22993129 DOI:

Sangseok L, Dong KL. What is the proper way to apply the multiple comparison test? Korean J Anesthesiol. 2018;71(5):353-60. PMid:30157585 DOI:

Subandrate, Faisal ME, Anggraini NW. The role of oxidative stress on pre-eclampsia. Mirror of Med. World. 2017;44(5):353-5.

Leonard SW, Traber MG. Methods for assessment of Vitamin E. In: Laboratory Assessment of Vitamin Status. Amsterdam, Netherlands: Elsevier Inc.; 2018. p. 79-105. DOI:

Zingg JM, Meydani M, Azzi A. α-Tocopheryl phosphate-An activated form of vitamin E important for angiogenesis and vasculogenesis? BioFactors. 2012;38(1):24-33. PMid:22281871 DOI:

Alcala M, Calderon-Dominguez M, Serra D, Herrero L, Ramos MP, Viana M. Short-term Vitamin E treatment impairs reactive oxygen species signaling required for adipose tissue expansion, resulting in fatty liver and insulin resistance in obese mice. PLoS One. 2017;12(10):e0186579. PMid:29028831 DOI:

Sakai C, Ishida M, Ohba H, Yamashita H, Uchida H, Yoshizumi M, et al. Fish oil omega-3 polyunsaturated fatty acids attenuate oxidative stress-induced DNA damage in vascular endothelial cells. PLoS One. 2017;12(11):e0187934. PMid:29121093 DOI:

Sley EG, Rosen EM, van‘t Erve TJ, Sathyanarayana S, Barrett ES, Nguyen RH, et al. Omega-3 fatty acid supplement use and oxidative stress levels in pregnancy. PLoS One. 2020;15(10):e0240244. PMid:33095772 DOI:

Mannaerts D, Faes E, Cos P, Briedé JJ, Gyselaers W, Cornette J, et al. Oxidative stress in healthy pregnancy and preeclampsia is linked to chronic inflammation, iron status and vascular function. PLoS One. 2018;13(9):e0202919. PMid:30204759 DOI:

Kasture V, Kale A, Randhir K, Sundrani D, Joshi S. Effect of maternal omega-3 fatty acids and vitamin E supplementation on placental apoptotic markers in rat model of early and late onset preeclampsia. Life Sci. 2019;239:117038. PMid:31730868 DOI:

Kasture VV, Sundrani DP, Joshi SR. Maternal one carbon metabolism through increased oxidative stress and disturbed angiogenesis can influence placental apoptosis in preeclampsia. Life Sci. 2018;206:61-9. PMid:29772225 DOI:

Vanitha, Dixit D, Sanikop A, Virupaxi RD. Expression of vascular endothelial growth factor A 165 (VEGF-A) in term normal placenta: Immunohistochemical study Int J Health Sci Res. 2018;8(8):110-4.

Tandon V, Hiwale S, Amle D, Nagaria T, Patra PK. Assessment of serum vascular endothelial growth factor levels in pregnancy-induced hypertension patients. J Pregnancy. 2017;2017:3179670. PMid:28133548 DOI:

Eddy AC, Iii GL, George EM. Pro-angiogenic Therapeutics for Preeclampsia. 2018. p. 1-11. DOI:

Ahmadi A, Gharipour M, Arabzadeh G, Moin P, Hashemipour M, Kelishadi R. The effects of Vitamin E and omega-3 PUFAs on endothelial function among adolescents with metabolic syndrome. Biomed Res Int. 2014;2014:906019. PMid:25136638 DOI:




How to Cite

Amir A, Darwin E. Omega-3 and Vitamin E Supplementation Effect on Reactive Oxygen Species and Placental Vascular Endothelial Growth Factor: Pre-eclampsia Model. Open Access Maced J Med Sci [Internet]. 2022 Jun. 16 [cited 2024 Mar. 5];10(A):1387-91. Available from:

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