Oxidative Stress in the Oral Cavity before and After Prosthetic Treatment

Zlatina Tomova; Desislav Tomov; Atanas Chonin; Iliyana Stoeva; Angelina Vlahova; Elena Vasileva

doi:10.3889/oamjms.2022.9960

Authors

Zlatina Tomova Department of Prosthetic Dental Medicine, Faculty of Dental Medicine, Medical University of Plovdiv, Plovdiv, Bulgaria
Desislav Tomov Department of Bioorganic Chemistry, Faculty of Pharmacy, Medical University of Plovdiv, Plovdiv, Bulgaria https://orcid.org/0000-0002-2028-9855
Atanas Chonin Department of Diagnostic Imaging, Dental Allergology and Physiotherapy, Faculty of Dental Medicine, Medical University of Plovdiv, Plovdiv, Bulgaria
Iliyana Stoeva Department of Diagnostic Imaging, Dental Allergology and Physiotherapy, Faculty of Dental Medicine, Medical University of Plovdiv, Plovdiv, Bulgaria
Angelina Vlahova Department of Prosthetic Dental Medicine, Faculty of Dental Medicine, Medical University of Plovdiv, Plovdiv, Bulgaria https://orcid.org/0000-0002-4794-2324
Elena Vasileva Department of Prosthetic Dental Medicine, Faculty of Dental Medicine, Medical University of Plovdiv, Plovdiv, Bulgaria https://orcid.org/0000-0002-2718-0094

DOI:

https://doi.org/10.3889/oamjms.2022.9960

Keywords:

Saliva, Isoprostanes, Base dental alloys

Abstract

BACKGROUND: Metal ions emitted from dental alloys may induce oxidative stress leading to numerous pathological changes. Lipid peroxidation may cause disturbance of structure and function of cell membranes, apoptosis, autophagy, and formation of potentially mutagenic compounds. Products of interaction between reactive oxygen species and biomolecules may be used for evaluation of oxidative stress level.

AIM: The aim of this study was to evaluate the influence of the prosthetic dental treatment with metal ceramic restorations on the level of oxidative stress in the oral cavity.

MATERIALS AND METHODS: Metal ceramic crowns with copings fabricated by direct metal laser sintering were produced for 35 patients. CoCr dental alloy EOS CobaltChrome SP2 (EOS) was used. Non-stimulated and stimulated saliva samples were collected from the patients before and after the prosthetic treatment. For evaluation of oxidative stress concentration of 8-isoPGF2-alpha was measured by liquid chromatography tandem mass spectrometry. For statistical processing, non-parametric Wilcoxon signed-rank test and Mann–Whitney test were applied.

RESULTS: The concentration of isoprostane 8-isoPGF2-alpha in non-stimulated saliva was lower 2 h after fixing the crowns compared to the initial level and statistically significant difference was observed. On the 7^thday the concentration of isoprostanes remained significantly lower than the initial one. No significant differences were found in isoprostane concentration in stimulated saliva before and after prosthetic treatment.

CONCLUSION: Prosthetic dental treatment leads to decrease in oral oxidative stress.

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References

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