Antibacterial Activity of Lemon Basil (Ocimum citriodorum) and Key Lime (Citrus aurantifolia S.) Extract Combination on Streptococcus mutants

Retno Dwi Sari; Junaidi Junaidi; Karin Tika Fitria

doi:10.3889/oamjms.2022.9978

Authors

Retno Dwi Sari Department of Dental Nursing, Politeknik Kesehatan Kementerian Kesehatan Jambi, Jambi, Indonesia
Junaidi Junaidi Department of Dental Nursing, Politeknik Kesehatan Kementerian Kesehatan Jambi, Jambi, Indonesia
Karin Tika Fitria Department of Dental Nursing, Politeknik Kesehatan Kementerian Kesehatan Jambi, Jambi, Indonesia

DOI:

https://doi.org/10.3889/oamjms.2022.9978

Keywords:

Lemon basil, Key lime, Kemangi, Jeruk nipis, Streptococcus mutans

Abstract

BACKGROUND: Lots of plants that are used in Southeast Asian and Indonesian cuisine have antibacterial properties.

AIM: This study aimed to find out the effectiveness of lemon basil (Ocimum citriodorum) and key lime (Citrus aurantifolia S.) extract combination on Streptococcus mutants’ inhibition.

METHODS: Lemon Basil and Key Lime were extracted and combined with several following concentration, respectively, (30–70%, 40–60%, 50–50%, 60–40%, and 70–30%). S. mutants were cultured using Brain-heart Infusion Broth overnight. The inhibition range from the extract combination was measured and analyzed.

RESULTS: All combinations except 60–40 showed significant effectivity against S. mutants compared to control (p < 0.05). Post hoc tests revealed that only the combination Lemon Basil-Key Lime 30–70% that has a significantly wider inhibition range compared to L50%-K50% (p = 0.034), L60%-K40% (p = 0.007), and L70%–K30% (p = 0.023).

CONCLUSION: Combination extract of Lemon Basil and Key lime has effectivity against oral micro bacteria, especially S. mutants.

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