Prophylactic and Curative Assessment of Essentiale ForteÂ® On Carbon Tetrachloride-Induced Liver Damage in Wistar Rats

Olaoluwa S Olukiran; Rufus O Akomolafe; Kayode O Bamitale; Akande O Ajayi; Raphael E Okonji; Ronald A Bejide

doi:10.3889/oamjms.2014.070

Authors

Olaoluwa S Olukiran Department of Physiological Sciences, Obafemi Awolowo University, Ile-Ife
Rufus O Akomolafe Department of Physiological Sciences, Obafemi Awolowo University, Ile-Ife
Kayode O Bamitale Department of Medical Pharmacology and Therapeutics, Obafemi Awolowo University, Ile-Ife
Akande O Ajayi Department of Medicine, College of Medicine, Ekiti State University, Ado-Ekiti
Raphael E Okonji Department of Biochemistry, Obafemi Awolowo University, Ile-Ife
Ronald A Bejide Department of Morbid Anatomy and Forensic Medicine, Obafemi Awolowo University, Ile-Ife

DOI:

https://doi.org/10.3889/oamjms.2014.070

Keywords:

Carbon tetrachloride, Curative, Essentiale forte, Prophylactic, Rat

Abstract

AIM: This study was to assess the prophylactic and curative effects of Essentiale forte (ESF) on carbon tetrachloride (CCl₄) induced liver damage in Wistar rats.

MATERIALS AND METHODS: Twenty-four adult Wistar rats were randomly divided into four groups of six rats each. Group I (control group) received 0.3 ml/kg/day of propylene glycol for one month; group II (toxic control) was given 0.7 ml/kg/day of CCl₄ dissolved in olive oil (1:1,v/v) orally for 7 days; group III (prophylactic group) received 4.3 mg/kg/day of ESF for one month followed by CCl₄ for one week; group IV (curative group) was treated with CCl₄ for one week and subsequently received ESF (4.3 mg/kg/day) for one month. Half of the rats were sacrificed at active period, the other half after a 2-week recovery period.

RESULTS: The activities of serum AST, ALT, ALP, total bilirubin level were significantly higher, total protein and GSH levels were significantly reduced in the toxic control group compared to the control group. Group III had significantly higher AST and ALT activities compared to the control rats at active period, whereas after the recovery period no significant differences were observed in almost all the parameters. Moreover, no significant differences in the parameters mentioned above were observed in group IV compared to the control rats at active and recovery period.

CONCLUSION: The results of this study showed that Essential forte was better as a curative agent rather than a prophylactic agent in rats.

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References

Navarro VJ, Senior JR. Drug-related hepatotoxicity. N Engl J Med. 2006; 35(4): 731-739. DOI: https://doi.org/10.1056/NEJMra052270

Subramoniam A, Pushpangadan P. Development of phytomedicine for liver diseases. Indian J Pharmacol. 1999; 31(3): 166-75.

Baranisrinivasan P, Elumalai EK, Sivakumar C, Theresa SV, David E. Hepatoprotective effect of Enicostemma Littorale blume and Eclipta alba during ethanol induced oxidative stress in albino rats. Inter J Pharmacol. 2009; 5(4): 268-72. DOI: https://doi.org/10.3923/ijp.2009.268.272

Marina N. Hepatotoxicity of antiretroviral: incidence, mechanisms and management. J Hepatol. 2006; 44(1): S132-S9. DOI: https://doi.org/10.1016/j.jhep.2005.11.027

Poongothai K, Karkuzhali G, Siva-Prakash T, Sangeetha T, Saravanan G, Deepa R, Gopalakrishnan S, Mohan V. Effect of Essentiale in diabetic subjects with Non-alcoholic fatty liver. Int J Diab Dev Countries. 2005; 25(12): 1-6. DOI: https://doi.org/10.4103/0973-3930.26859

Ovchinnikov AA, Tsvettsikh VE, Berdichevskii BA, Lykov VN, Suktanbaev VR, Murychev, AV. â€œ Essential phospholipids in the treatment of chronic pyelonephritisâ€. Urol Nefrol (Mosk). 1996; 4:7-9.

Habbu PV, Shastry RA, Mahadevan KM, Joshi H, Das SK. Hepatoprotective and antioxidant effects of Argyreia Speciosa in rats. Afr J Tradit Complement Altern Med. 2008; 5: 158-164. DOI: https://doi.org/10.4314/ajtcam.v5i2.31268

Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein - dye binding. Anal Biochem. 1976; 72: 248-54. DOI: https://doi.org/10.1016/0003-2697(76)90527-3

Tietze F. Enzymatic method for the quantative determination of nanogram amount of total and oxidized glutathione applications to mammalian blood and other tissues. Anal Biochem. 1969; 27: 502-22. DOI: https://doi.org/10.1016/0003-2697(69)90064-5

Jayakumar T, Sakthivel M, Thomas PA, Geraldine P. Pleurotus ostreatus, an oyster mushroom, decreases the oxidative stress induced by carbon tetrachloride in rat kidneys, heart and brain. Chem Biol Interact 2008; 176(2-3): 108-20. DOI: https://doi.org/10.1016/j.cbi.2008.08.006

Guyton AC, Hall JE. Textbook of Medical Physiology. 10th edn. Harcourt International Edition. Published by W.B. Saunders Company, Philadelphia, PA, pp 734-862, 2001.

Naik SR, Panda VS. Hepatoprotective effect of Ginkgoselect phytosome in rifampicin induced liver injury in rats. Fitoterapia 2008; 79-439-45. DOI: https://doi.org/10.1016/j.fitote.2008.02.013

Rajesh MG, Latha MS. Premilinary evaluations of the antihepatotoxic effect of Kamilari, a polyherbal formulation. J Ethnopharmacol 2004; 91: 99-104. DOI: https://doi.org/10.1016/j.jep.2003.12.011

Thabrew MI, Joice PDTM, Rajatissa W. A comparative study of the efficacy of Pavetta indica and Osbecka octandra in the treatment of liver dysfunction. Planta Med 1987; 53: 239-241. DOI: https://doi.org/10.1055/s-2006-962691

Wallnoefer H, Hanusch M. Essential phospholipids in the treatment of hepatic disease. Med Monatsschr 1973; 27: 131-6.

Pratt DS, Kaplan MM. Laboratory tests. In: Schiff ER, Sorell MF, Maddrey WC eds. Schiffâ€™s diseases of the liver. 8th ed. Philadelphia: Lippincott-Raven, 1: 205-44, 1999.

Rosen HR, Keefe EB. Evaluation of abnormal liver enzymes, use of liver tests and the serology of viral hepatitis: Liver disease, diagnosis and management. 1st ed. New York; Churchill livingstone publishers, 24-35, 2000.

Redinger RN, Small DM. Bile composition, bile salt metabolism and gallstones. Arch Intern Med 1972; 130 (4): 618-630. DOI: https://doi.org/10.1001/archinte.130.4.618

Clawson GA. Mechanisms of carbon tetrachloride hepatotoxicity. Pathol Immunopathol Res 1989; 8:104- 112. DOI: https://doi.org/10.1159/000157141

Muriel P, Alba N, Perez-Alvarez VM et al. Kupffer cells inhibition prevents hepatic lipid peroxidation and damage induced by carbon tetrachloride. Comp Biochem Physiol C Toxicol Pharmacol 2001; 130: 219-226. DOI: https://doi.org/10.1016/S1532-0456(01)00237-X

Havel RJ. Functional activities of hepatic lipoproteins receptors. Ann Rev Physiol 1986; 48: 119-134. DOI: https://doi.org/10.1146/annurev.ph.48.030186.001003

Lii CK, Ko YJ, Chiang MT, Sung WC, Hen HW. Effect of dietary vitamin E on antioxidant status and antioxidant enzyme activities in Sprague-Dawley rats. Nutr Cancer 1998; 32: 95-100. DOI: https://doi.org/10.1080/01635589809514725

Pompella A, Visvikis A, Paolicchi A, de Tata V, Casim AF. The changing faces of glutathione, a cellular protagonist. Biochem Pharmacol 2003; 66 (8):1499-503. DOI: https://doi.org/10.1016/S0006-2952(03)00504-5

Brigelius â€“ Flohe R. Vitamin E: the shrew waiting to be tamed. Free Radic Biol Med 2009; 46: 543-54. DOI: https://doi.org/10.1016/j.freeradbiomed.2008.12.007