HLA Genotyping in Patients with End-Stage Renal Disease Waiting For Cadaveric Renal Transplantation in Federation of Bosnia and Herzegovina

Elma FejziÄ‡; Jasenko KaramehiÄ‡; Izet EminoviÄ‡; Damir SuljeviÄ‡; Andi AlijagiÄ‡; Semir BeÄ‡ireviÄ‡; Amela Å ahoviÄ‡; Sanela Å iÅ¡iÄ‡

doi:10.3889/oamjms.2017.015

Authors

Elma FejziÄ‡ Institute of Transfusion Medicine of Federation of Bosnia and Herzegovina, Sarajevo
Jasenko KaramehiÄ‡ Department of Immunology, Clinical Center University of Sarajevo, Sarajevo
Izet EminoviÄ‡ Department of Biochemistry and Physiology, Faculty of Science, University of Sarajevo, Sarajevo
Damir SuljeviÄ‡ Department of Biochemistry and Physiology, Faculty of Science, University of Sarajevo, Sarajevo
Andi AlijagiÄ‡ Department of Biochemistry and Physiology, Faculty of Science, University of Sarajevo, Sarajevo
Semir BeÄ‡ireviÄ‡ Institute of Pathology, Clinical Center University of Tuzla, Tuzla
Amela Å ahoviÄ‡ Institute of Transfusion Medicine of Federation of Bosnia and Herzegovina, Sarajevo
Sanela Å iÅ¡iÄ‡ Institute of Transfusion Medicine of Federation of Bosnia and Herzegovina, Sarajevo

DOI:

https://doi.org/10.3889/oamjms.2017.015

Keywords:

Major Histocompatibility Complex (MHC), Human Leukocyte Antigens (HLA), Allele groups, Genotype, Haplotype

Abstract

AIM: The research was conducted by genotyping two Human Leukocyte Antigen (HLA) gene classes. The main objective of this research was to investigate distribution and frequency of the allelic groups, genotypes and haplotypes in the gene loci of HLA class I (HLA-A*, -B*, -C*) and HLA class II (HLA-DRB1*, -DQB1*) in patients included in the program of cadaveric renal transplantation.

MATERIAL AND METHODS: Our study covered 186 blood samples of patients who are registered on the list for cadaveric renal transplantation in Federation of Bosnia and Herzegovina and included 59 control, healthy unrelated individuals. For the HLA typing, we have used three different methods: micro lymphocyte cytotoxicity test (MLCT), Polymerase Chain Reaction (PCR) â€“ Sequence Specific Primers (SSP) and PCR â€“ Sequence-Specific Oligonucleotides (SSO) or Luminex technology. All patients and cadaveric donors were tested using the three methods because the system is polymorphic.

RESULTS: Analysis of the results of genotyping HLA class I gene loci identified dominant HLA-A*02, HLA-B*35, HLA-C*07 allelic groups. Analysis of the HLA class II gene loci genotyping showed that HLA-DRB1*11 and HLA-DQB1*03 loci had the highest incidence in HLA class II.

CONCLUSION: Based on our results and previous research, there were no observed differences between allelic frequencies and genotypes of healthy people and people with ESRD. Differences between allelic groups occurred, but they were not statistically significant, except HLA-C*01 (p = 0.020).

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