Serum VEGF Levels in Helicobacter pylori Infection and Correlation with Helicobacter pylori cagA and vacA Genes

Gontar Siregar; Dina Sari; Taufik Sungkar

doi:10.3889/oamjms.2017.031

Authors

Gontar Siregar University of Sumatera Utara, Gastroentero-Hepatology, Medan, Sumatera Utara 20122
Dina Sari University of Sumatera Utara, Nutrition, Medan, Sumatera Utara
Taufik Sungkar University of Sumatera Utara, Gastroentero-Hepatology, Medan, Sumatera Utara 20122

DOI:

https://doi.org/10.3889/oamjms.2017.031

Keywords:

Helicobacter pylori, Vascular endothelial growth factor, cagA gene, vacA gene, Gastritis

Abstract

BACKGROUND: Helicobacter pylori vacA and cagA genes are associated with higher virulence. Vascular Endothelial Growth Factor (VEGF) is one important marker for neo-angiogenesis.

AIM: The purpose of this study was to investigate the relationship between VEGF serum levels with cagA and vacA genes in H. pylori infection.

METHODS: A cross-sectional study was done on eighty patients that consecutive admitted to endoscopy unit. The diagnosis of H. pylori infection was based on rapid urease test. Serum samples were obtained to determine circulating VEGF level. Polymerase chain reaction was done to examine H. pylori vacA and cagA genes. Data analysis were carried-out using SPSS version 22.Â

RESULTS: A total of 80 patients were examined. There were 45 (56.3%) patients infected with Helicobacter pylori. There were 33 (73.3%) patients with H. pylori cagA positive. Serum VEGF levels in patients with the H. pylori positive were significantly higher compared to the patients that have no H. pylori. Serum levels of VEGF were significantly higher in cagA positive than negative.

CONCLUSION: Serum VEGF level is correlated with H. pylori infection and its virulence status. The more virulence of H. pylori, cagA gene, the higher serum VEGF levels were found.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

Plum Analytics Artifact Widget Block

References

Sipponen P, Maaroos HI. Chronic gastritis. Scandinavian Journal of Gastroenterology. 2015;50:657-67. PMid:25901896 PMCid:PMC4673514 DOI: https://doi.org/10.3109/00365521.2015.1019918

Varbanova M, FrauenschlÃ¤ger K, Malfertheiner P. Chronic gastritis â€“ An update. Best Practice & Research Clinical Gastroenterology. 2014;28:1031-42. https://doi.org/10.1016/j.bpg.2014.10.005 PMid:25439069 DOI: https://doi.org/10.1016/j.bpg.2014.10.005

Rehnberg-Laiho L, Rautelin H, Koskela P, Sarna S, Pukkala E, Aromaa A, et al. Decreasing prevalence of helicobacter antibodies in Finland, with reference to the decreasing incidence of gastric cancer. Epidemiol Infect. 2001;126:37-42. PMid:11293681 PMCid:PMC2869672 DOI: https://doi.org/10.1017/S0950268801005027

Matsukura N, Yamada S, Kato S, Tomtitchong P, Tajiri T, Miki M, et al. Genetic differences in interleukin-1 beta polymorphisms among four Asian populations: an analysis of the Asian paradox between H. pylori infection and gastric cancer incidence. J Exp Clin Cancer Res. 2003; 22: 47-55. PMid:12725322

Rugge M, Genta RM. Staging and grading of chronic gastritis. Human pathology. 2005;36:228-33. https://doi.org/10.1016/j.humpath.2004.12.008 PMid:15791566 DOI: https://doi.org/10.1016/j.humpath.2004.12.008

Tuccillo C, Cuorno A, Rocco A, Martinelli E, Staibano S, Mascolo M, et al. Vascular endothelial growth factor and neo-angiogenesis in H. pylori gastritis in humans. J Pathol. 2005;207: 277â€“84. https://doi.org/10.1002/path.1844 PMid:16184519 DOI: https://doi.org/10.1002/path.1844

Caputo R, Tuccillo C, Manzo BA, Zarrilli R, Tortora G, Blanco CDV, et al. Helicobacter pylori VacA Toxin Up-Regulates Vascular Endothelial Growth Factor Expression in MKN 28 Gastric Cells through an Epidermal Growth Factor Receptor-, Cyclooxygenase-2-dependent Mechanism. Clin Cancer Res. 2003;9:2015-21. PMid:12796363

Rugge M, Pennelli G, Pilozzi E, et al. Gastritis: the histology report. Dig Liver Dis. 2011;43S:S373â€“84. https://doi.org/10.1016/S1590-8658(11)60593-8 DOI: https://doi.org/10.1016/S1590-8658(11)60593-8

Rojborwonwitaya J, Vijitjunykul N. Comparison of the Accuracy of Two Commercial Rapid Urase Tests, CLOtestÂ® and Pronto DryÂ®, in detecting Helicobacter pylori Infection. Thai J Gastroenterol. 2005:6(2):55-60.

Salimzadeh L, Bagheri N, Zamanzad B, et al. Frequency of virulence factors in Helicobacter pylori-infected patients with gastritis. Microbiol Pathogenesis. 2015;30:1-6. https://doi.org/10.1016/j.micpath.2015.01.008 DOI: https://doi.org/10.1016/j.micpath.2015.01.008

Vannarath S, Vilaichone RK, Rasachak B, et al. Virulence genes of Helicobacter pylori in gastritis, peptic ulcer and gastric cancer in Laos. Asian Pac J Cancer Prev. 2014;15(20):9027-31. https://doi.org/10.7314/APJCP.2014.15.20.9027 PMid:25374247 DOI: https://doi.org/10.7314/APJCP.2014.15.20.9027

Myint T, Shiota S, Vilaichone RK, et al. Prevalence of Helicobacter pylori infection and atrophic gastritis in patients with dyspeptic symptoms in Myanmar. World J Gastroenterol. 2015;21(2):629-36. https://doi.org/10.3748/wjg.v21.i2.629 PMid:25605987 PMCid:PMC4296025 DOI: https://doi.org/10.3748/wjg.v21.i2.629

Syam AF, Miftahussur M, Makmun D, et al. Risk factors and prevalence of Helicobacter pylori in five largest islands of Indonesia: A preliminary study. PLoS ONE. 2015: 1-14. https://doi.org/10.1371/journal.pone.0140186 DOI: https://doi.org/10.1371/journal.pone.0140186

Kang MJ, Song EJ, Kim BY, et al. Helicobacter pylori induces vascular endothelial growth factor production in gastric epithelial cells through hypoxia-inducible factor-1Î±-dependent pathway. Helicobacter. 2014;19:476-83. https://doi.org/10.1111/hel.12169 PMid:25231285 DOI: https://doi.org/10.1111/hel.12169

Yamaoka Y, Graham DY. Helicobacter pylori virulence and cancer pathogenesis. Future Oncol. 2014;10(8):1487-500. https://doi.org/10.2217/fon.14.29 PMid:25052757 PMCid:PMC4197059 DOI: https://doi.org/10.2217/fon.14.29

Yakut M, Ã–rmenci N, Erdal H, et al. The association between precancerous gastritis lesions and serum pepsinogens, serum gastrin, vascular endothelial growth factor, serum interleukin-1 Beta, serum toll-like receptor-4 levels and Helicobacter pylori Cag a status. Clinics and Research in Hepatology and Gastroenterology. 2014;37:302-11. https://doi.org/10.1016/j.clinre.2012.09.013 PMid:23137754 DOI: https://doi.org/10.1016/j.clinre.2012.09.013

Trang TTH, shiota S, Matsuda M, et al. The prevalence of Helicobacter pylori virulence factors in Bhutan, Vietnam, and Myanmar is related to Gastric Cancer Incidence. BioMed Research International. 2015;2015:1-8. https://doi.org/10.1155/2015/830813 PMid:26090448 PMCid:PMC4450262 DOI: https://doi.org/10.1155/2015/830813

Atherton JC, Cover TL, Papini E, Telford JL. Vacuolating Cytotoxin. In: Mobley HLT, Mendz GL, Hazell SL eds. Helicobacter pylori. Physiology and Genetics, 2001: 97â€“109. https://doi.org/10.1128/9781555818005.ch9 DOI: https://doi.org/10.1128/9781555818005.ch9

Siregar GA, Halim S, Sitepu VR. Serum TNF-Î±, Il-8, VEGF levels in Helicobacter pylori infection and their association with degree of gastritis. Acta Med Indones. 2015;47(2):120-6. PMid:26260554