Utility of Tissue Inhibitor Metalloproteinase-1 and Osteopontin as Prospective Biomarkers of Early Cardiovascular Complications in Type 2 Diabetes

Doaa Samir Salah El-Din; Ahmed Ibrahim Amin; Ahmed Osman Egiza

doi:10.3889/oamjms.2018.081

Authors

Doaa Samir Salah El-Din Chemistry, Faculty of Science, Cairo University, Giza 12613
Ahmed Ibrahim Amin Chemistry, Faculty of Science, Cairo University, Giza 12613
Ahmed Osman Egiza Chemistry, Faculty of Science, Cairo University, Giza 12613

DOI:

https://doi.org/10.3889/oamjms.2018.081

Keywords:

Molecular markers, expression level, tissue inhibitor metalloproteinase-1, OPN concentrations, diabetic cardiovascular diseases

Abstract

AIM: This work investigated associations between tissue inhibitor metalloproteinase-1 and diabetic cardiovascular diseases in type 2 diabetic patients; also it investigated the role of osteopontin in the diagnosis of type 2 cardiovascular diabetes complications.

SUBJECTS AND METHODS: These were examined on eighty subjects, divided into three groups as follows: twenty volunteer healthy control subjects, thirty type 2 diabetes mellitus (DM) patients, and thirty cardiovascular, diabetic patients. Full clinical measurements were carried out, and the expression level of tissue inhibitor metalloproteinase-1 in blood samples was analysed by real-time PCR, using gene-specific primer pairs. Also osteopontin concentrations had been measured by the enzyme-linked immunosorbent assay. Data were tested statistically by parametric tests.

RESULTS: The concentrations of osteopontin and the expression levels of tissue inhibitor metalloproteinase-1 were significantly increased in diabetic and cardiovascular diabetic groups compared to control group also they were significantly increased in the cardiovascular diabetic group compared to the diabetic group.

CONCLUSION: Tissue inhibitor metalloproteinase-1 and osteopontin concentrations were significantly increased in diabetic patients with cardiovascular complications than other groups.

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