Impact of Silver Nanoparticles on Gene Expression in Aspergillus Flavus Producer Aflatoxin B1


  • Mohamed Mahmoud Deabes Food Toxicology & Contaminants Department, National Research Centre, 33 Bohouth St., 12622 Dokki, Giza
  • Wagdy Khalil Bassaly Khalil Cell Biology Department, National Research Centre, 33 Bohouth St., 12622 Dokki, Giza
  • Ashraf Gamil Attallah Microbial Genetics Department, National Research Centre, 33 Bohouth St., 12622 Dokki, Giza
  • Tarek Ahmed El-Desouky Food Toxicology & Contaminants Department, National Research Centre, 33 Bohouth St., 12622 Dokki, Giza
  • Khayria Mahmoud Naguib Food Toxicology & Contaminants Department, National Research Centre, 33 Bohouth St., 12622 Dokki, Giza



Aflatoxin B1, Silver nanoparticles, qRT-PCR, HPLC, Omt-A gene


AIM: In this study, we evaluated the effect of silver nanoparticles (AgNPs) on the production of aflatoxin B1 (AFB1) through assessment the transcription activity of aflatoxin biosynthesis pathway genes in Aspergillus flavus ATCC28542.

MATERIAL AND METHODS: The mRNAs were quantitative by Real Time-polymerase chain reaction (qRT-PCR) of A. flavus grown in yeast extract sucrose (YES) medium containing AgNPs. Specific primers that are involved in the AFB1 biosynthesis which highly specific to A. flavus, O-methyltransferase gene (omt-A), were designed and used to detect the fungus activity by quantitative PCR assay. The AFB1 production (from A. flavus growth) which effected by AgNPs were measured in YES medium by high-pressure liquid chromatography (HPLC).

RESULTS: The AFB1 produced by A. flavus have the highest reduction with 1.5 mg -100 ml of AgNPs were added in media those records 88.2%, 67.7% and 83.5% reduction by using AgNP HA1N, AgNP HA2N and AgNP EH, respectively. While on mycelial growth give significantly inhibitory effect. These results have been confirmed by qRT-PCR which showed that culture of A. flavus with the presence of AgNPs reduced the expression levels of omt-A gene.

CONCLUSION: Based on the results of the present study, AgNPs inhibit growth and AFB1 produced by Aspergillus flavus ATCC28542. This was confirmed through RT-PCR approach showing the effect of AgNPs on omt-A gene involved in aflatoxin biosynthesis.


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How to Cite

Deabes MM, Khalil WKB, Attallah AG, El-Desouky TA, Naguib KM. Impact of Silver Nanoparticles on Gene Expression in Aspergillus Flavus Producer Aflatoxin B1. Open Access Maced J Med Sci [Internet]. 2018 Apr. 13 [cited 2023 Feb. 4];6(4):600-5. Available from:



A - Basic Science