The The Content of Extracellular Nucleic Acids in the Blood and Ejaculate of Men of Reproductive Age Living in the Ecologically Unfavourable Regions of the Aral Sea

Svetlana Jangildinova; Yelena Tatina; Gulzhan Kaliyeva; Kuttykyz Kuvatbaeva; Nursaya Beygam

doi:10.3889/oamjms.2019.273

Authors

Svetlana Jangildinova Department of Biology, Karaganda Medical University, Karaganda, Kazakhstan
Yelena Tatina Department of Biology, Karaganda Medical University, Karaganda, Kazakhstan
Gulzhan Kaliyeva Department of Biology, Karaganda Medical University, Karaganda, Kazakhstan
Kuttykyz Kuvatbaeva Department of Biology, Karaganda Medical University, Karaganda, Kazakhstan
Nursaya Beygam Department of Biology, Karaganda Medical University, Karaganda, Kazakhstan

DOI:

https://doi.org/10.3889/oamjms.2019.273

Keywords:

Extracellular DNA, Men of reproductive age, Ejaculate, Plasma acid soluble fractions (ASF), Plasma RNA, Plasma DNA

Abstract

AIM: We aimed to study the influence of adverse environmental factors on molecular-cellular processes in the population living in the Aral Sea region.

METHODS: Extracellular RNA (ecRNA) and ecDNA were determined in blood. We obtained the ejaculate of the studied men after 4-5 days of abstinence. The ejaculate was placed in a warm tube with a ground stopper. The examination of the ejaculate was started in 20-30 minutes after receiving, as during this time it is subjected to liquefaction. Spectrophotometry of ASF, RNA and DNA hydrolysates was performed on an SF 26 at a wavelength of 290 nm against H₂O.

RESULTS: In the ejaculate of the studied groups of men, significant deviations in the content of extracellular nucleic acid fractions from the indicators of the comparison group were also detected. Statistically significant differences in the content of extracellular RNA were observed in men of the younger age group living in the territory of all study regions. A significant increase in the content of extracellular DNA was detected in two regions, but not in all age groups.

CONCLUSION: The study revealed a significant increase in the content of extracellular nucleic acids in the biological fluids of men of reproductive age living in the Aral Sea region. The most significant are the changes in the level of extracellular RNA in the blood plasma and ejaculate in men of the younger age group and the increase in ASF content in the ejaculate in men of all age groups.