Influence of Flavonoids on the Cytotoxic Activity of Mononuclear Blood Cells in Model Tests

Liudmila Ivanovna Babaskina; Tatiana Mikhailovna Litvinova; Dmitrii Vladimirovich Babaskin; Mikhail Valentinovich Kiselevsky; Olga Vladimirovna Savinova; Elizaveta Aleksandrovna Winter

doi:10.3889/oamjms.2019.331

Authors

Liudmila Ivanovna Babaskina Sechenov First Moscow State Medical University, 8-2 Trubetskaya Street, Moscow, Russian Federation
Tatiana Mikhailovna Litvinova Sechenov First Moscow State Medical University, 8-2 Trubetskaya Street, Moscow, Russian Federation
Dmitrii Vladimirovich Babaskin Sechenov First Moscow State Medical University, 8-2 Trubetskaya Street, Moscow, Russian Federation
Mikhail Valentinovich Kiselevsky N. N. Blokhin National Medical Research Center of Oncology, Ministry of Health of Russia, 24 Kashirskoe Highway, Moscow, Russian Federation
Olga Vladimirovna Savinova Sechenov First Moscow State Medical University, 8-2 Trubetskaya Street, Moscow, Russian Federation
Elizaveta Aleksandrovna Winter Sechenov First Moscow State Medical University, 8-2 Trubetskaya Street, Moscow, Russian Federation

DOI:

https://doi.org/10.3889/oamjms.2019.331

Keywords:

flavonoids, cytotoxic activity, plant extract, cytotoxic test, mononuclear blood cells

Abstract

BACKGROUND: The spread of phytocomplex application and justification of its selective effects on tumour cells (mainly due to the presence of flavonoids) require research of its cytotoxic and immunomodulatory activity.

AIM: The goal was to study the direct cytotoxic effect of the phytocomplex and its modulating effect on the cytotoxic activity of the donor's mononuclear blood cells in in vitro experiments.

METHODS: The phytocomplex was a dry extract from marsh cinquefoil, creeping alfalfa and common hop; its main active ingredients were flavonoids. Transplantable monolayer cultures of lung adenocarcinoma, colorectal cancer, erythroblastic leukaemia, and fibroblasts were used as target cells. The cytotoxic activity was assessed using a cytotoxic test based on the selective ability to live cells to reduce MTT (3-[4, 5-dimethyltriazol-2-yl]-2, 5 diphenyltetrazolium bromide) to formazan in mitochondria. Quantitative determination of formazan was performed using spectrophotometry.

RESULTS: A direct cytotoxic effect of the phytocomplex in concentrations of at least 2.5 mg/ml on tumour cells has been established. Its modulating effect on the cytotoxic activity of mononuclear blood cells at a concentration of 0.05 mg/ml was shown. The phytocomplex in doses of 0.25 and 0.5 mg/ml increased the killer activity of the mononuclear cells in a diseased person's blood, but did not affect these blood cells in a healthy donor. Incubation of lymphocytes with a phytocomplex for 24 hours increased the cytotoxic activity of mononuclear cells by 20-25%.

CONCLUSION: The direct cytotoxic effect of the phytocomplex and its modulating effect on the cytotoxic activity of mononuclear blood cells in model experiments in vitro have been established.