Toxicopathological, Cytogenetic Effects of Acetothioamide on Female Albino Mice Reproductive System

Anas A.  Humadi; Bushra I.  AL-Kaisei; Taghreed J.  Humadai; Ali Ibrahim Ali AL-Ezzy

doi:10.3889/oamjms.2019.858

Authors

Anas A. Humadi Department of Pathology, College of Veterinary Medicine, University of Diyala, Diyala, Iraq
Bushra I. AL-Kaisei Department of Pathology and Poultry Disease, College of Veterinary Medicine, University of Baghdad, Baghdad, Iraq
Taghreed J. Humadai Department of Pathology and Poultry Disease, College of Veterinary Medicine, University of Baghdad, Baghdad, Iraq
Ali Ibrahim Ali AL-Ezzy Department of Pathology, College of Veterinary Medicine, University of Diyala, Diyala, Iraq

DOI:

https://doi.org/10.3889/oamjms.2019.858

Keywords:

Acetothioamide, Toxicopathological effects, cytogenetic effects, female reproductive system

Abstract

AIM: To determine toxicopathological and cytogenetic effects of Acetothioamide (ATA) on the female reproductive system.

METHODS: Twenty albino female mice were divided equally into two groups: the first group (control) fed with diet pellet. The second group (treated) were inoculated intraperitoneally with a single dose of ATA (100 mg/kg Bw) for 15 days. All mice were sacrificed at the end of the experiment and blood was collected for evaluation of (FSH and LH), serum peroxy nitrate radical concentration. Cytogenetic analysis (chromosomal aberration, micronuclei, mitotic index and blast index) and the histopathological examination on ovary and uterus were done.

RESULTS: ATA causes significant reduction (p < 0.0001) for FSH, LH and serum peroxy nitrate radical concentration among treated females. Oophoritis, pyometria, thrombosis and endometrial hyperplasia with granulomatous reaction were reported among treated females mainly in uterus tissue and ovary.

CONCLUSION: ATA causes significant reduction for FSH, LH and serum peroxy nitrate concentration among treated females. Oophoritis, pyometria, thrombosis and endometrial hyperplasia with granulomatous reaction were the main pathological changes in uterus tissue and ovary among treated females.