In Vitro Antioxidant Activity and In Vivo Hepatoprotective Effects of Ethanolic Extracts from Wall-Broken Ganoderma Lucidum Spores

Authors

  • Nguyen Huu Lac Thuy Faculty of Pharmacy, University of Medicine and Pharmacy, Ho Chi Minh City, Viet Nam https://orcid.org/0000-0001-7585-6469
  • Vo Thi Diem Faculty of Pharmacy, University of Medicine and Pharmacy, Ho Chi Minh City, Viet Nam
  • Trinh Thi Dieu Thuong Faculty of Traditional Medicine, University of Medicine and Pharmacy, Ho Chi Minh City, Viet Nam
  • Tran Tuyet Anh Faculty of Pharmacy, University of Medicine and Pharmacy, Ho Chi Minh City, Viet Nam
  • Truong Minh Nhut Faculty of Pharmacy, University of Medicine and Pharmacy, Ho Chi Minh City, Viet Nam
  • Truong Van Dat Faculty of Pharmacy, University of Medicine and Pharmacy, Ho Chi Minh City, Viet Nam
  • Huynh Ngoc Trinh Faculty of Pharmacy, University of Medicine and Pharmacy, Ho Chi Minh City, Viet Nam https://orcid.org/0000-0003-2473-4482

DOI:

https://doi.org/10.3889/oamjms.2022.10421

Keywords:

Antioxidant, Cyclophosphamide, Ethanolic extracts, Total triterpenoid, Wall-broken Ganoderma lucidum spores

Abstract

The wall-broken Ganoderma lucidum spores are widely used in recent years in the belief that active components inside the spores are better released and well absorbed when taken orally. In this study, the sporoderm of G. lucidum was broken by autoclaving at a high temperature. The powder of wall-broken spores was then extracted by soaking with ethanol at different concentrations (50 %, 70 % and 96 %). The 70% and 50% ethanol extracts had the highest total triterpenoid content, in which ganoderic acid A was predominant. In the DPPH free radical scavenging test, 70% ethanol extract exhibited the highest in vitro antioxidant activity. This 70% ethanol extract was also safe in mice at the dose of 2,000 mg/kg body weight. Moreover, this extract protected the liver from acute injury induced by cyclophosphamide. Indeed, the pretreatment by oral administration of 70% ethanol extract prevented serum ALT and AST activities elevation and attenuated hepatic MDA formation and GSH depletion following administration of cyclophosphamide in mice.

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Published

2022-09-05

How to Cite

1.
Thuy NHL, Diem VT, Thuong TTD, Anh TT, Nhut TM, Dat TV, Trinh HN. In Vitro Antioxidant Activity and In Vivo Hepatoprotective Effects of Ethanolic Extracts from Wall-Broken Ganoderma Lucidum Spores. Open Access Maced J Med Sci [Internet]. 2022 Sep. 5 [cited 2024 Mar. 29];10(A):1450-5. Available from: https://oamjms.eu/index.php/mjms/article/view/10421

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