The Effect of Three Different Biomaterials on Proliferation and Viability of Human Dental Pulp Stem Cells (In-vitro Study)

Dalia A. Mohamed; Maha I. Abdelfattah; Eman H. A. Aboulezz

doi:10.3889/oamjms.2017.089

Authors

Dalia A. Mohamed Endodontic Department, Faculty of Dentistry Suez Canal University
Maha I. Abdelfattah Oro-dental Genetics Department, Human Genetics and Genome Research Division, National Research Centre, Cairo
Eman H. A. Aboulezz Basic Dental Science Department, Oro-dental Research Division, National Research Centre, Cairo

DOI:

https://doi.org/10.3889/oamjms.2017.089

Keywords:

Cytotoxicity, Nano-hydroxy apatite (NHA), Mineral trioxide aggregate (MTA), calcium-enriched mixture (CEM), dental pulp stem cells (DPSCs)

Abstract

BACKGROUND: Biomaterial cytotoxicity on dental stem cells plays a critical role in managing the regeneration of dental tissue.

AIM: The aim of the present study was to evaluate the effect of Nano-hydroxy apatite (NHA), Mineral trioxide aggregate (MTA), and Calcium-enriched mixture (CEM) on the proliferation, and viability of human dental pulp stem cells (hDPSCs) isolated from third molar teeth.

METHODS: Cultured DPSCs were characterized and the tested biomaterials were shaped into cylinders then inserted directly on the DPSCs. Proliferation and viability percentage of DPSCs were evaluated at 1, 3, 5, 7, 9, 11, and 14 days of culture.

RESULTS: The biomaterials supplemented DPSCs showed a significant initial decrease in cell count and viability percentage at day one. Then, a rise in cell counts and viabilities was noticed after that. There was a decrease in cell counts, and viabilities in the NHA supplemented cells in comparison to other tested biomaterials.

CONCLUSIONS: All tested biomaterials maintain the proliferation of DPSCs for different durations. NHA showed less proliferative and more cytotoxic effect than other tested materials.

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References

Sloan AJ, Waddington RJ. Dental pulp stem cells: What, where, how? Int J Paediatr Dent. 2009;19(1):61-70. https://doi.org/10.1111/j.1365-263X.2008.00964.x PMid:19120509

Gomes Cornelio AL, Salles LP, Campos Da Paz M, Cirelli JA, Guerreiro-Tanomaru JM, Tanomaru Filho M. Cytotoxicity of Portland cement with different radiopacifying agents: A cell death study. J Endod. 2011;37(2):203-10. https://doi.org/10.1016/j.joen.2010.11.017 PMid:21238803

Kim S, Jeon M, Shin DM, Lee JH, Song JS. [Effects of mineral trioxide aggregate on the proliferation and differentiation of human pulp cells from primary and permanent teeth]. Korean Acad Pediatr Dent. 2013;40:185-193. https://doi.org/10.5933/JKAPD.2013.40.3.185

Minamikawa H, Yamada M, Deyama Y, Suzuki K, Kaga M, Yawaka Y, et al. Effect of N-acetylcysteine on rat dental pulp cells cultured on mineral trioxide aggregate. J Endod [Internet]. 2011;37(5):637-41. https://doi.org/10.1016/j.joen.2011.02.012 PMid:21496663

Utneja S, Nawal RR, Talwar S, Verma M. Current perspectives of bio-ceramic technology in endodontics: calcium enriched mixture cement - review of its composition, properties and applications. Restor Dent Endod. 2015;40(1):1. https://doi.org/10.5395/rde.2015.40.1.1 PMid:25671207 PMCid:PMC4320271

Hosseinzade M, Soflou RK, Valian A, Nojehdehian H. Physicochemical properties of MTA, CEM, hydroxyapatite and nano hydroxyapatite-chitosan dental cements. Biomed Res. 2016;27(2):442-8.

Xu Z, Liu C, Wei J, Sun J. Effects of four types of hydroxyapatite nanoparticles with different nanocrystal morphologies and sizes on apoptosis in rat osteoblasts. J Appl Toxicol [Internet]. 2012;32(6):429-35. https://doi.org/10.1002/jat.1745 PMid:22162110

Vitti RP, Prati C, Sinhoreti MAC, Zanchi CH, Souza E Silva MG, Ogliari FA, et al. Chemical-physical properties of experimental root canal sealers based on butyl ethylene glycol disalicylate and MTA. Dent Mater. 2013;29(12):1287-94. https://doi.org/10.1016/j.dental.2013.10.002 PMid:24183503

Abdelfattah MI, Hassib NF. The efficacy of vitamin C in the formation of periodontal ligament stem cell sheet. Egypt J Histol. 2015;38(4):837-43. https://doi.org/10.1097/01.EHX.0000475425.12581.33

Ahmed NE-MB, Aboul-Ezz EHA, Zakhary SY, El Badry TH, Ramzy MI. Isolation of Dental Pulp Stem Cells and their In Vitro Differentiation into Odontoblast-like Cells. Maced J Med Sci [Internet]. 2011;4(3):253-60. https://doi.org/10.3889/MJMS.1857-5773.2011.0142

Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 2001;25(4):402-8. https://doi.org/10.1006/meth.2001.1262 PMid:11846609

Samyuktha V, Ravikumar P, Nagesh B, Ranganathan K, Jayaprakash T, Sayesh V. Cytotoxicity evaluation of root repair materials in human-cultured periodontal ligament fibroblasts. J Conserv Dent 2014;17(5):467. https://doi.org/10.4103/0972-0707.139844 PMid:25298650 PMCid:PMC4174709

Gronthos S, Mankani M, Brahim J, Robey PG SS. Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo. Proc Natl Acad Sci USA. 2000;97:13625-30. https://doi.org/10.1073/pnas.240309797 PMid:11087820 PMCid:PMC17626

Abdelfattah MI, Nasry SA, Mostafa AA. Characterization and cytotoxicity analysis of a ciprofloxacin loaded Chitosan/Bioglass scaffold on cultured human periodontal ligament stem cells: A preliminary report. Open Access Maced J Med Sci. 2016;4(3):461-7. https://doi.org/10.3889/oamjms.2016.052 PMid:27703576 PMCid:PMC5042636

Schneider R, Rex Holland G, Chiego D, Hu JCC, Nor JE, Botero TM. White Mineral Trioxide Aggregate Induces Migration and Proliferation of Stem Cells from the Apical Papilla. J Endod. 2014;40(7):931-6. https://doi.org/10.1016/j.joen.2013.11.021 PMid:24935538 PMCid:PMC4426880

Margunato S1, Tasl? PN2, Ayd?n S2, Karap?nar Kazandag M1 SF. In Vitro Evaluation of ProRoot MTA, Biodentine, and MM-MTA on Human Alveolar Bone Marrow Stem Cells in Terms of Biocompatibility and Mineralization. J Endod. 2015;41(10):1646-52. https://doi.org/10.1016/j.joen.2015.05.012 PMid:26410417

Jaberiansari Z, Naderi S, FST. Cytotoxic Effects of Various Mineral Trioxide Aggregate Formulations, Calcium-Enriched Mixture and a New Cement on Human Pulp Stem Cells. Iran Endod J. 2014;9(4):271-276. PMid:25386208 PMCid:PMC4224765

Hengameh A, Reyhaneh D, Nima MM, Hamed H. Effects of two bioactive materials on survival and osteoblastic differentiation of human mesenchymal stem cells. J Conserv Dent. 2014;17(4):349-353. https://doi.org/10.4103/0972-0707.136509 PMid:25125848 PMCid:PMC4127694

Er K, Polat ZA, Ozan F, Tasdemir T, Sezer U, Siso SH. Cytotoxicity analysis of strontium ranelate on cultured human periodontal ligament fibroblasts: a preliminary report. J Formos Med Assoc. 2008;107(8):609-15. https://doi.org/10.1016/S0929-6646(08)60178-3

Gomes-Cornelio AL, Rodrigues EM, Salles LP, Mestieri LB, Faria G, Guerreiro-Tanomaru JMT-FM. Bioactivity of MTA Plus, Biodentine and an experimental calcium silicate-based cement on human osteoblast-like cells. Int Endod J. 2017; 50AD:1(39-47).

Saeed Asgary, a Laleh Alim Marvasti, b and AK. Indications and Case Series of Intentional Replantation of Teeth. Iran Endod J. 2014;9(1):71-78. PMid:24396380

Salles LP, Gomes-Cornelio AL, Guimaraes FC, Herrera BS, Bao SN, Rossa-Junior C, et al. Mineral trioxide aggregate-based endodontic sealer stimulates hydroxyapatite nucleation in human osteoblast-like cell culture. J Endod. 2012;38(7):971-6. https://doi.org/10.1016/j.joen.2012.02.018 PMid:22703663

Bin CV, Valera MC, Camargo SEA, Rabelo SB, Silva GO, Balducci I, et al. Cytotoxicity and Genotoxicity of Root Canal Sealers Based on Mineral Trioxide Aggregate. J Endod. 2012;38(4):495-500. https://doi.org/10.1016/j.joen.2011.11.003 PMid:22414836

Liu X, Zhao M, Lu J, Ma J, Wei J, Wei S. Cell responses to two kinds of nanohydroxyapatite with different sizes and crystallinities. Int J Nanomedicine. 2012;7:1239-50. https://doi.org/10.2147/IJN.S28098 PMid:22419871 PMCid:PMC3299575

Mozayeni MA, Milani AS, Marvasti LA, Asgary S. Cytotoxicity of calcium enriched mixture cement compared with mineral trioxide aggregate and intermediate. Aust Endo J. 2012;38:70-5. https://doi.org/10.1111/j.1747-4477.2010.00269.x PMid:22827819

Saberi EA, Karkehabadi HMN. Cytotoxicity of Various Endodontic Materials on Stem Cells of Human Apical Papilla. Iran Endod J. 2016;11(1):17-22. PMid:26843872

Zhao X, Ng S, Heng BC, Guo J, Ma L, Tan TTY, et al. Cytotoxicity of hydroxyapatite nanoparticles is shape and cell dependent. Arch Toxicol. 2013;87(6):1037-52. https://doi.org/10.1007/s00204-012-0827-1 PMid:22415765

Xiaochen Liu, Minzhi Zhao, Jingxiong Lu, Jian Ma, Jie Wei and SW. Cell responses to two kinds of nanohydroxyapatite with different sizes and crystallinities. Int J Nanomedicine. 2012;7:1239-1250. https://doi.org/10.2147/IJN.S28098 PMid:22419871 PMCid:PMC3299575

Soheilipour E, Kheirieh S, Madani M, Baghban AA, Asgary S. Particle size of a new endodontic cement compared to Root MTA and calcium hydroxide. Iran Endod J. 2009;4(3):112-116. PMid:24003332 PMCid:PMC3758862

Ghoddusi J, Afshari JT, Donyavi Z, Brook A, Disfani R, Esmaeelzadeh M. Cytotoxic effect of a new endodontic cement and mineral trioxide aggregate on L929 line culture. Iran Endod J. 2008;3(2):17-23. PMid:24171015 PMCid:PMC3808562

Semeghini MS, Fernandes RR, Chimello DT, Oliveira FS B-PK. In vitro evaluation of the odontogenic potential of mouse undifferentiated pulp cells. Braz Dent J. 2012;23(4):328-36. https://doi.org/10.1590/S0103-64402012000400004 PMid:23207845