Characterization and Cytotoxicity Analysis of a Ciprofloxacin Loaded Chitosan/Bioglass Scaffold on Cultured Human Periodontal Ligament Stem Cells: a Preliminary Report
DOI:
https://doi.org/10.3889/oamjms.2016.052Keywords:
stem cells, periodontal, cytotoxicity, ciprofloxacin, chitosanAbstract
AIM: The aim of this study was to analyze the cytotoxicity of ciprofloxacin (CIP) loaded on chitosan bioactive glass scaffold on human periodontal ligament stem cells (PLSCs) in vitro.
MATERIALS AND METHODS: PLSCs obtained from human third molars, cultures treated with medium containing 15 x 15 mm chitosan/bioactive glass scaffolds without/with different concentration 0, 5, 10, and 20 % of CIP. A total of 15 x 10^3 cells were plated in 6 well plates. The attached cells of each group were harvested from the plates after 1, 4 and 8 days of culture to detect the viability of cells. The cell number was determined using a hemocytometer and the trypan blue dye-exclusion assay. Data was analyzed using normality using Shapiro-Wilk test. Comparisons between groups were made using One-way ANOVA complemented by Tukey's test.
RESULTS: When comparing the proliferation rate of cells in the four groups, no statistically significant difference was found (P = 0.633). With regards to cell viability, no statistical difference was found between the 0, 5, and 10 % CIP concentrations, while the 20 % CIP concentration demonstrated the least viability with a high statistically significant difference (P = 0.003).
CONCLUSION: Twenty percentages CIP demonstrated the least proliferation rate and viability.Downloads
Metrics
Plum Analytics Artifact Widget Block
References
Bali D, Pandit N, Kathuria R, Bali A. Genetics and Aggressive Periodontal Disease : An Update Review. J Oral Heal Community Dent. 2012;6:97–101.
Leid JG. Bacterial Biofilms Resist Key Host Defenses. Microbe. 2009;4(2):66–70.
Mukherjee S(1) KD. Study of neutrophils isolated from peripheral blood of patients suffering from aggressive periodontitis at the cellular level: Receptors and cytoskeletal reorganization. J Indian Soc Periodontol. 2012;16(1):59–64. http://dx.doi.org/10.4103/0972-124X.94606 PMid:22628965 PMCid:PMC3357037
Thanakun S, Pornprasertsuk-Damrongsri S, Gokyu M, Kobayashi H, Izumi Y. Inverse Association of Plasma IgG Antibody to Aggregatibacter actinomycetemcomitans and High C-Reactive Protein Levels in Patients with Metabolic Syndrome and Periodontitis. PLoS One. 2016;11(2):e0148638. http://dx.doi.org/10.1371/journal.pone.0148638 PMid:26871443 PMCid:PMC4752452
Jepsen K, Jepsen S. Antibiotics / antimicrobials : systemic and local administration in the therapy of mild to moderately advanced periodontitis. Periodontol 2000. 2016;71:82–112. http://dx.doi.org/10.1111/prd.12121 PMid:27045432
Johnson JD, Chen R, Lenton PA, Zhang G, Hinrichs JE, Rudney JD. Persistence of extracrevicular bacterial reservoirs after treatment of aggressive periodontitis. J Periodontol. 2008;79(12):2305–12. http://dx.doi.org/10.1902/jop.2008.080254 PMid:19053921 PMCid:PMC2597013
Semwal A, Singh R, Dutta PK. Chitosan: A Promising Substrate for Pharmaceuticals. J Chitin Chitosan Sci. 2013;1(2):87–102. http://dx.doi.org/10.1166/jcc.2013.1012
Pichayakorn W, Boonme P. Evaluation of cross-linked chitosan microparticles containing metronidazole for periodontitis treatment. Mater Sci Eng C Mater Biol Appl. 2013 1;33(3):1197–202.
Yao Q, Li W, Yu S, Ma L, Jin D, Boccaccini AR, et al. Multifunctional chitosan/polyvinyl pyrrolidone/45S5 Bioglass® scaffolds for MC3T3-E1 cell stimulation and drug release. Mater Sci Eng C Mater Biol Appl. 2015 1;56:473–80. http://dx.doi.org/10.1016/j.msec.2015.06.046 PMid:26249617
Suci P, Young M. Selective killing of Aggregatibacter actinomycetemcomitans by ciprofloxacin during development of a dual species biofilm with Streptococcus sanguinis. Arch Oral Biol. 2011;56(10):1055–63. http://dx.doi.org/10.1016/j.archoralbio.2011.03.013 PMid:21507381
Prakasam A, Elavarasu SS, Natarajan RK. Antibiotics in the management of aggressive periodontitis. Journal of pharmacy & bioallied sciences. 2012; 4:252–5. http://dx.doi.org/10.4103/0975-7406.100226 PMid:23066264 PMCid:PMC3467876
Tezel A, Yucel O, Orbak R, Kara C, Kavrut F, Yagiz H, et al. The gingival crevicular fluid ciprofloxacin level in subjects with gingivitis and periodontitis, and its effects on clinical parameters. J Periodontal Res. 2005;40(5):395–400. http://dx.doi.org/10.1111/j.1600-0765.2005.00820.x PMid:16105092
Romorini L, Riva DA, Blüguermann C, Videla Richardson GA, Scassa ME, Sevlever GE, et al. Effect of antibiotics against Mycoplasma sp. on human embryonic stem cells undifferentiated status, pluripotency, cell viability and growth. PLoS One. 2013;8(7):e70267. http://dx.doi.org/10.1371/journal.pone.0070267 PMid:23936178 PMCid:PMC3728093
Chuensombat S, Khemaleelakul S, Chattipakorn S, Srisuwan T. Cytotoxic effects and antibacterial efficacy of a 3-antibiotic combination: an in vitro study. J Endod. 2013;39(6):813–9. http://dx.doi.org/10.1016/j.joen.2012.11.041 PMid:23683284
Ruparel NB, Teixeira FB, Ferraz CCR, Diogenes A. Direct effect of intracanal medicaments on survival of stem cells of the apical papilla. J Endod. 2012;38(10):1372–5. http://dx.doi.org/10.1016/j.joen.2012.06.018 PMid:22980180
Mabrouk M, Mostafa A, Oudadesse H, Wers E, Lucas-Girot MIE-G A. Study of Nanobioactive Glass Quaternary System 46S6. Bioceram Dev Appl. 2014;4:1000072.
Mabrouk M, Mostafa AA, Oudadesse H, Mahmoud AA, Gaafar AM, El-Gohary MI. Fabrication, Characterization and Drug Release of Ciprofloxacin Loaded Porous Polyvinyl Alcohol / Bioactive Glass Scaffold for Controlled Drug Delivery. Bioceram Dev Appl. 2013;S1(1):2–5.
Mabrouk M, Mostafa AA, Oudadesse H, Mahmoud AA, El-Gohary MI. Bioactivity and drug delivering ability of a chitosan/46S6 melted bioactive glass biocomposite scaffold. Int Ceram Rev. 2013;62(6):444–52.
Mostafa AA, Zaazou MH, Chow LC, Mahmoud AA, Zaki DY, Basha M, et al. Injectable nanoamorphous calcium phosphate based in situ gel systems for the treatment of periapical lesions. Biomed Mater. 2015;10(6):065006. http://dx.doi.org/10.1088/1748-6041/10/6/065006 PMid:26541174
Liu J, Wang L, Liu W, Li Q, Jin Z, Jin Y. Dental follicle cells rescue the regenerative capacity of periodontal ligament stem cells in an inflammatory microenvironment. PLoS One. 2014;9(9):e108752. http://dx.doi.org/10.1371/journal.pone.0108752 PMid:25275580 PMCid:PMC4183515
Lee S-Y, Chiang P-C, Tsai Y-H, Tsai S-Y, Jeng J-H, Kawata T, et al. Effects of cryopreservation of intact teeth on the isolated dental pulp stem cells. Journal of Endodontics. 2010; 36:1336–40. http://dx.doi.org/10.1016/j.joen.2010.04.015 PMid:20647092
Perry BC, Zhou D, Wu X, Yang F-C, Byers M a, Chu T-MG et al. Collection, cryopreservation, and characterization of human dental pulp-derived mesenchymal stem cells for banking and clinical use. Tissue Eng Part C Methods. 2008;14(2):149–56. http://dx.doi.org/10.1089/ten.tec.2008.0031 PMid:18489245 PMCid:PMC2963629
Kamocki K, Nör JE, Bottino MC. Effects of ciprofloxacin-containing antimicrobial scaffolds on dental pulp stem cell viability-In vitro studies. Arch Oral Biol. 2015;60(8):1131–7. http://dx.doi.org/10.1016/j.archoralbio.2015.05.002 PMid:26042622
Er K, Polat ZA, Özan F, Taşdemir T, Sezer U, Siso SH. Cytotoxicity analysis of strontium ranelate on cultured human periodontal ligament fibroblasts: A preliminary report. J Formos Med Assoc. 2008;107(8):609–15. http://dx.doi.org/10.1016/S0929-6646(08)60178-3
Kaplish V, Walia MK, Kumar SLH. Pharmacophore. Pharmacophore. 2013;4(2):39–49.
Koort JK, Makinen TJ, Suokas E, Veiranto M, Jalava J, Tormala P, et al. Sustained release of ciprofloxacin from an osteoconductive poly(DL)-lactide implant. Acta Orthop. 2008;79(2):295–301. http://dx.doi.org/10.1080/17453670710015111 PMid:18484258
Joshi D, Garg T, Goyal AK, Rath G. Advanced drug delivery approaches against periodontitis. Drug Deliv. 2016;23(2):363–77. http://dx.doi.org/10.3109/10717544.2014.935531 PMid:25005586
Roshna T, Nandakumar K. Generalized Aggressive Periodontitis and Its Treatment Options: Case Reports and Review of the Literature. Case Rep Med. 2012;2012:1–17. http://dx.doi.org/10.1155/2012/535321 PMid:22291715 PMCid:PMC3265097
Yang S, Leong KF, Du Z, Chua CK. The design of scaffolds for use in tissue engineering. Part I. Traditional factors. Tissue Eng. 2001;7(6):679–89. http://dx.doi.org/10.1089/107632701753337645 PMid:11749726
Mochizuki M, Kadoya Y, Wakabayashi Y, Kato K, Okazaki I, Yamada M, et al. Laminin-1 peptide-conjugated chitosan membranes as a novel approach for cell engineering. FASEB J. 2003;17(8):875–7. http://dx.doi.org/10.1096/fj.02-0564fje
Catauro M, Renella RA, Papale F, Vecchio Ciprioti S. Investigation of bioactivity, biocompatibility and thermal behavior of sol-gel silica glass containing a high PEG percentage. Mater Sci Eng C Mater Biol Appl. 2016 1;61:51–5. http://dx.doi.org/10.1016/j.msec.2015.11.077 PMid:26838823
RodrÃguez-Lozano FJ, GarcÃa-Bernal D, Aznar-Cervantes S, Ros-Roca MA, Algueró MC, Atucha NM, et al. Effects of composite films of silk fibroin and graphene oxide on the proliferation, cell viability and mesenchymal phenotype of periodontal ligament stem cells. J Mater Sci Mater Med. 2014;25(12):2731–41. http://dx.doi.org/10.1007/s10856-014-5293-2 PMid:25081645
Downloads
Published
How to Cite
Issue
Section
License
http://creativecommons.org/licenses/by-nc/4.0
