Overview of MDM2 and B-RAF Expression in Gastric Lesions


  • Tarek Aboushousha Department of Pathology, Theodor Bilharz Research Institute, Imbaba, Giza
  • Noha Helal Department of Pathology, Theodor Bilharz Research Institute, Imbaba, Giza
  • Olfat Hammam Faculty of Biotechnology, October University of Modern Sciences and Arts, Giza
  • Manar Ibrahim Faculty of Biotechnology, October University of Modern Sciences and Arts, Giza
  • Samar Khaled Faculty of Biotechnology, October University of Modern Sciences and Arts, Giza
  • Amr Mostafa Department of Surgery, Theodor Bilharz Research Institute, Imbaba, Giza
  • Amgad Anas Department of Hepato-Gastroenterology, Theodor Bilharz Research Institute, Giza




MDM2, B-RAF, Gastritis, Gastric cancer, H. pylori


BACKGROUND: Globally, gastric cancer (GC) it is the fourth most common cancer and the third cause of cancer-related deaths. Overexpression of MDM2 and B-RAF appeared to be increased in malignancy and associated with poor prognosis in several human tumours, but their role in gastric cancer remains controversial.

AIM: We had investigated the immunohistochemical expression of MDM2 and B-RAF in 136 gastric lesions with/without H. pylori association.

MATERIAL AND METHODS: Studied specimens include chronic gastritis (32), intestinal type GC (70), diffuse GC (22) and gastrointestinal stromal tumours (GIST) (12).

RESULTS: MDM2 expression increased significantly in intestinal GC compared to other groups (p < 0.001), while B-RAF expression increased significantly in GIST compared to other groups (p < 0.001). H. pylori increased expression of MDM2 in intestinal GC cases but did not affect B-RAF expression. MDM2 expression correlated with high grade of tumor differentiation (p < 0.001), deep invasion (p < 0.05), nodal metastases (p < 0.05) and distant metastases (p < 0.1) in intestinal GC, while B-RAF expression did not correlate with TNM stage (p < 0.1).

CONCLUSION: MDM2 up-regulation was more frequent in intestinal GC, while B-RAF up-regulation was more frequent in GIST compared to other groups; MDM2 expression in intestinal GC was correlated with H. pylori association, high grade of differentiation, deep invasion, nodal and distant metastases, meanwhile, B-RAF expression was correlated with high-grade intestinal GC but did not correlate with H. pylori or TNM stage. The possible role of both MDM2 and B-RAF in predicting progression of gastric tumours and prognosis deserves further investigations.


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How to Cite

Aboushousha T, Helal N, Hammam O, Ibrahim M, Khaled S, Mostafa A, Anas A. Overview of MDM2 and B-RAF Expression in Gastric Lesions. Open Access Maced J Med Sci [Internet]. 2018 Oct. 10 [cited 2021 Apr. 12];6(10):1795-802. Available from: https://oamjms.eu/index.php/mjms/article/view/oamjms.2018.338



A - Basic Science

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