Influence of Combined Therapy on Generation of Neutrophil Extracellular Traps in Patients with Cervical Cancer

Yuriy Fomenko; Yevgeniya Kolesnikova; Irina Beynikova; Larissa Muravlyova; Valentina Sirota; Ryszhan Bakirova

doi:10.3889/oamjms.2018.483

Authors

Yuriy Fomenko Department of Oncology, Karaganda State Medical University, Karaganda, Kazakhstan
Yevgeniya Kolesnikova Department of the Biological Chemistry, Karaganda State Medical University, Karaganda, Kazakhstan
Irina Beynikova Department of the Biological Chemistry, Karaganda State Medical University, Karaganda, Kazakhstan
Larissa Muravlyova Department of the Biological Chemistry, Karaganda State Medical University, Karaganda, Kazakhstan http://orcid.org/0000-0002-6997-2268
Valentina Sirota Department of Oncology, Karaganda State Medical University, Karaganda, Kazakhstan
Ryszhan Bakirova Department of Internal Diseases, Karaganda State Medical University, Karaganda, Kazakhstan

DOI:

https://doi.org/10.3889/oamjms.2018.483

Keywords:

Neutrophil Extracellular Traps, NETs, Cervical Cancer

Abstract

BACKGROUND: According to several authors, neutrophil extracellular traps (NETs) play an important role in the mechanisms of cancer development and metastatic processes, which allows them to be considered as a potential new target for the treatment of cancer.

AIM: To investigate the presence of extracellular neutrophil traps in the blood of patients with cervical cancer on the background of the combined treatment.

MATERIALS AND METHODS: The study was conducted in 28 patients with cervical cancer. Group 1 received only radiation therapy; Groups 2-radiation therapy with ftorafur; Group 3-radiation therapy with cisplatin. To determine the number of spontaneous extracellular neutrophilic traps in the blood of the examined individuals, we used a technique of I.I. Dolgushin and Yu.S. Andreeva.

RESULTS: Peripheral blood neutrophils in 53.57% (33.87; 72.49) of cervical cancer patients showed the ability to generate NETs before treatment. The ability to form NETs was observed in neutrophils isolated from 66.67% (9.43; 99.16) patients of the Group 1. After radiation therapy with ftorafur, the ability of blood neutrophils to form NETs was observed in 50% (1.26; 98.74) of cervical cancer patients. After radiotherapy with cisplatin, 37.50% (15.20; 64.57) of patients were found to have NETs formation

CONCLUSION: The ability to form NETs varied greatly after radiotherapy. The addition of chemotherapy drugs to radiation therapy did not increase the percentage of NETs in the blood of patients with cervical cancer but stimulated the appearance of basophil extracellular traps.

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