A Promising Probiotic Irrigant: An In Vitro Study


  • Hoda El-sayed National Research Centre, Dairy Science Department (Microbiology Lab.), Food Industries and Nutrition Division, Giza, Egypt
  • Yousra Aly National Research Centre, Restorative Dentistry and Dental Material Department, Giza, Egypt
  • Hanaa Elgamily National Research Centre, Restorative Dentistry and Dental Material Department, Giza, Egypt
  • Mohamed M. Nagy Endodontic Department, Faculty of Dentistry, Ain Shams University, Cairo, Egypt




Probiotics, Microbial ecology, Lactobacillus rhamnosus, Sodium hypochlorite, Colony counting, Enterococcus faecalis


AIM: The present study aimed to investigate the inhibitory effect of Lactobacillus rhamnosus (B-445) as a probiotics irrigant on the growth of Enterococcus faecalis.

METHODS: Forty-two extracted single human canal anterior teeth were prepared with rotary instrumentation and sterilised. Teeth were divided into 3 groups according to the type of irrigant, N = 14. Three experimental groups were inoculated with E. faecalis and cultured for 21 days before use; Group 1 was 2.5% NaOCl (positive control), Group 2 was saline (negative control), Group 3 was the experimental probiotic irrigant. Paper point sampling of the canals of each group was obtained before irrigation (S1), immediately after irrigation (S2) and after 24 hours (post irrigation samples) (S3) to determine remaining colony forming units for E. faecalis. Also, Colony counts for L. rhamnosus in Group 3 after immediate irrigation, as well as 24 hours post irrigation, was performed to determine the survival profile of these bacteria in infected root canal with E. faecalis.

RESULTS: The NaOCl irrigant group had the lowest mean value of (log 10 CFU/mL) of E. faecalis after immediate irrigation and after 24 hrs post irrigation followed by the probiotic group, while the highest mean value was the saline group (P ≤ 0.001). The survival profile for L. rhamnosus in Group 3 after immediate irrigation and post-irrigation were slightly higher than for E. faecalis (P ≤ 0.001).

CONCLUSION: Lactobacillus rhamnosus which revealed a potential inhibitory effect on the growth of Enterococcus faecalis, could be used as a new natural, safe probiotic irrigant agent.

h of Enterococcus faecalis, could be used as a new natural, safe probiotic irrigant agent.


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How to Cite

El-sayed H, Aly Y, Elgamily H, Nagy MM. A Promising Probiotic Irrigant: An In Vitro Study. Open Access Maced J Med Sci [Internet]. 2019 Jan. 27 [cited 2024 Jun. 15];7(3):407-11. Available from: https://oamjms.eu/index.php/mjms/article/view/oamjms.2019.074



D - Dental Sciences

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