The The Influence of Mesenchymal Stem Cell Wharton Jelly toward Prostaglandin E2 Gene Expression on Synoviocyte Cell Osteoarthritis

Vivi Sofia; Moch Saiful Bachri; Rizki Rahmadian

doi:10.3889/oamjms.2019.082

Authors

Vivi Sofia Faculty of Pharmacy Ahmad Dahlan University, Jogjakarta, Indonesia
Moch Saiful Bachri Faculty of Pharmacy Ahmad Dahlan University, Jogjakarta, Indonesia
Rizki Rahmadian Department of Chemistry, Faculty of Medicine, Andalas University, Padang, Indonesia

DOI:

https://doi.org/10.3889/oamjms.2019.082

Keywords:

Co-culture, Prostaglandin E2, Mesenchymal Stem Cells

Abstract

BACKGROUND: Pharmacological therapy in the management of OA causes many new health problems due to side effects caused by long-term use of drugs, such as long-term use of Non-Steroidal Anti-Inflammatory Drugs (NSAIDs) will cause gastric ulcers and impaired kidney function. In OA pathogenesis, PGE2 gene is involved in the inflammation process.

AIM: This study aims to identify the influence of Wharton Jelly Mesenchymal Stem Cell (MSC-WJ) on PGE2 expression gene in synoviocyte by in vitro.

MATERIAL AND METHODS: The method used in this study is the co-culture method of primary cells and stem cells in the appropriate media. This research is pure experimental research. The sample used came from synovial tissue of osteoarthritis patients who underwent Total Knee Replacement (TKR) surgery. This study was divided into 6 groups treated with 4 replications. The expression analysis of the Prostaglandin E2 gene was done using qPCR (Real-Time Polymerase Chain Reaction). The expression analysis of the Prostaglandin E2 gene was carried out before and after the co-culture with Wharton's Jelly and continued with the analysis of statistical data processing using the SPSS.15 program. PGE2 gene expression data were processed using the Kruskal-Wallis test and continued with the Mann-Whitney test with a 95% confidence level.

RESULTS: The results showed that Mesenchymal Stem Cells Wharton Jelly could reduce the expression of Prostaglandin E2 gene after co-culture for 24 hours and 48 hours in synoviocyte cells osteoarthritis significantly compared with the control group. The administration of Mesenchymal Stem Cells for 24 hours reduced the expression level of PGE2 gene by 0.61 times compared to the control group (p < 0.05) and the administration of Mesenchymal Stem Cells for 48 hours decreased the expression level of PGE2 gene by 0, 47 times compared to the control group (p < 0.05).

CONCLUSION: This study concluded that MSC-WJ in OA synoviocyte significantly reduced the expression of the PGE2 gene (p < 0.05).

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

Plum Analytics Artifact Widget Block

References

WHO, 2004. The global burden of disease 2004 Up-date. WHO Press, Switzerland, 2004.

Hardy MM, Seibert K, Manning PT, Currie MG, Woerner BM, Edwards D, Koki A, Tripp CS. Cyclooxygenase 2â€dependent prostaglandin E2 modulates cartilage proteoglycan degradation in human osteoarthritis explants. Arthritis & Rheumatism: Official Journal of the American College of Rheumatology. 2002; 46(7):1789-803. https://doi.org/10.1002/art.10356 PMid:12124863

Shimpo H, Sakai T, Kondo S, Mishima S, Yoda M, Hiraiwa H et al. Regulation of prostaglandin E2 synthesis in cells derived from chondrocytes of patients with osteeoarthritis. Journal of Orthopaedic Science. 2009; 15:611-617. https://doi.org/10.1007/s00776-009-1370-7 PMid:19802674

Demoor M, Ollitrault D, Gomez-Leduc T, Bouyoucef M, Hervieu M, Fabre H, Lafont J, Denoix JM, Audigie F, Mallein-Gerin F, Legendre F. Cartilage tissue engineering: molecular control of chondrocyte differentiation for proper cartilage matrix reconstruction. Biochimica et Biophysica Acta (BBA)-General Subjects. 2014; 1840(8):2414-40. https://doi.org/10.1016/j.bbagen.2014.02.030 PMid:24608030

Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2âˆ’ Î”Î”CT method. methods. 2001; 25(4):402-8. https://doi.org/10.1006/meth.2001.1262 PMid:11846609

Razali NM and Wah YB. Power comparison of Saphiro Wilk, Kolmogorov- Smirnov, Lilefors and Anderson Drling-test. Faculty of Computer and Mathematical Science, University Technology MARA, 2017.

Shikichi M, Kitamura HP, Yanase H, Konno A, Takahashi-Iwanaga H, Iwanaga T. Three-dimensional ultrastructure of synoviocytes in the horse joint as revealed by the scanning electron microscope. Arch Histol Cytol. 1999; 62(3):219-29. https://doi.org/10.1679/aohc.62.219 PMid:10495876

Felson DT. Osteoarthritis of the knee. NEJM. 2006; 354:841-8. https://doi.org/10.1056/NEJMcp051726 PMid:16495396

Rosengren S, Boyle DL, Firestein GS. Acquisition, Culture and Phenotyping of Synovial Fibroblast. Methods in Molecular Medicine. 2007; 135:365-75. https://doi.org/10.1007/978-1-59745-401-8_24 PMid:17951672

Trzaska KA, Kuzhikandathil EV, Rameshwar P. Specification of a dopaminergic phenotype from adult human mesenchymal stem cells. Stem Cells. 2007; 25:2797-2808. https://doi.org/10.1634/stemcells.2007-0212 PMid:17656644

Listyorini D. Kultur Jaringan Hewan. FMIPA UM, 2001.

Tornatore L, Thotakura AK, Bennett J, Moretti M, Franzoso G. The nuclear factor kappa B signaling pathway: integrating metabolism with inflammation. Trends in cell biology. 2012; 22(11):557-66. https://doi.org/10.1016/j.tcb.2012.08.001 PMid:22995730

Kota DJ, Prabhakara KS, Todelano FN, Bhattarai D, Chen Q, DiCarlo B et al. Prostaglandin E2 indicates therapeutic efficacy of Mesenchymal Stem Cells In Experimental Traumatic Brain Injury. Stem Cells. 2017; 35(5):1416-1430. https://doi.org/10.1002/stem.2603 PMid:28233425

Xiaomin Lu, Jibin Han, Xiuping Xu, Jingyuan Xu, Ling Liu et al. PGE2 Promotes the Migration of Mesenchymal Stem Cells throught the Activation of FAK and ERK1/2 Pathway. Stem Cells Int. 2017; 8178643. https://doi.org/10.1155/2017/8178643

Kode JA, Mukherjee S, Joglekar MV, Hardikar AA. Mesenchymal stem cells: immunobiology and role in immunomodulation and tissue regeneration. Cytotherapy. 2009; 11(4):377-91. https://doi.org/10.1080/14653240903080367 PMid:19568970

Stappenbeck TS, Miyoshi H. The role of stromal stem cells in tissue regeneration and wound repair. Science. 2009; 324(5935):1666-9. https://doi.org/10.1126/science.1172687 PMid:19556498

Alvarez-Viejo M, et al. Quantifying mesenchymal stem cells in the mononuclear cell fraction of bone marrow samples obtained for cell therapy. Trans Proc. 2013; 45(1):434-439. https://doi.org/10.1016/j.transproceed.2012.05.091 PMid:23375334

Deng P, Chen QM, Hong C, Wang CY. Histone methyltransferases and demethylases: regulators in balancing osteogenic and adipogenic differentiation of mesenchymal stem cells. International journal of oral science. 2015; 7(4):197. https://doi.org/10.1038/ijos.2015.41 PMid:26674421 PMCid:PMC5153596

Wen L, Zhu M, Petsoglou C. Differentiation and immunomodulatory effects of bone marrow-derived mesenchymal stem cells on human corneal epithelium. Chin J Cell Stem Cell. 2014; 4(1):5-15.

Ryan AE, Lohan P, O'flynn L, Treacy O, Chen X, Coleman C, Shaw G, Murphy M, Barry F, Griffin MD, Ritter T. Chondrogenic differentiation increases antidonor immune response to allogeneic mesenchymal stem cell transplantation. Mol Ther. 2014; 22(3):655-67. https://doi.org/10.1038/mt.2013.261 PMid:24184966 PMCid:PMC3944342

Van Buul GM, Villafuertes E, Bos PK, Waarsing JH, Kops N, Narcisi R, Weinans H, Verhaar JA, Bernsen MR, Van Osch GJ. Mesenchymal stem cells secrete factors that inhibit inflammatory processes in short-term osteoarthritic synovium and cartilage explant culture. Osteoarthritis and Cartilage. 2012; 20(10):1186-96. https://doi.org/10.1016/j.joca.2012.06.003 PMid:22771777