Neuroprotective Activity of Evolvulus alsinoides & Centella asiatica Ethanolic Extracts in Scopolamine-Induced Amnesia in Swiss Albino Mice

Mukesh Kumar Yadav; Santosh Kumar Singh; Manish Singh; Shashank Shekhar Mishra; Anurag Kumar Singh; Jyoti Shankar Tripathi; Yamini Bhusan Tripathi

doi:10.3889/oamjms.2019.247

Authors

Mukesh Kumar Yadav Department of Kayachikitsa, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India
Santosh Kumar Singh Centre of Experimental Medicine & Surgery, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India
Manish Singh Department of Pharmacology, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India
Shashank Shekhar Mishra Department of Vikriti Vigyan, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India
Anurag Kumar Singh Centre of Experimental Medicine & Surgery, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India
Jyoti Shankar Tripathi Department of Kayachikitsa, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India
Yamini Bhusan Tripathi Department of Medicinal Chemistry, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India

DOI:

https://doi.org/10.3889/oamjms.2019.247

Keywords:

Centella asiatica, Evolvulus alsinoides L., Ethanolic extracts, In vivo antioxidant, Neuroprotective, Acetylcholine esterase

Abstract

AIM: To carry out the comparative nootropic, neuroprotective potentials of two medicinal plant species.

MATERIAL AND METHODS: For neuroprotective activity; behavior models (elevated plus maze & morris water maze), in vivo antioxidant (superoxide dismutase, catalase, lipid peroxidation & reduced glutathione), inflammatory markers (IL-1Î², IL-6 & TNF-Î±) and acetylcholine esterase (AChE) assessment procedures followed at different dosages i.e. 250 & 500 mg/kg of Evolvulus alsinoides and Centella asiatica ethanolic extracts. At the end of the study, it was performed histopathological analysis of the following organs: brain, heart, liver, and kidney.

RESULTS: In oral administration of different doses of ethanolic extracts of both medicinal plants i.e. Sco + EEA 250 = 2.49 Â± 0.29 , Sco + EEA 500 = 2.67 Â± 0.36, Sco + ECA 250 = 2.33 Â± 0.17, Sco + ECA 500 = 2.77 Â± 0.21, Sco + EEA + ECA 250 = 2.61 Â± 0.32 and Sco + EEA + ECA 500 = 2.79 Â± 0.16 U/mg of protein respectively against the scopolamine induced group Sco (control) = 5.51 Â± 0.35 U/mg of protein extracts shows neuroprotective and nootropic activity with reducing AChE level in the brain homogenate of swiss albino mice.

CONCLUSION: Since the E. alsinoides & C. asiatica are already used in traditional Indian medicine as the neuroprotective agent and also found promising effects over inflammatory diseases, wound healing, and immunomodulatory activity. The neuroprotective effect of both plants extracts attributed to inhibition of AChE activity and improve the spatial memory formation.

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