Efficiency of Crystal Violet Stain to Study Mitotic Figures in Oral Epithelial Dysplasia
DOI:
https://doi.org/10.3889/oamjms.2019.330Keywords:
Crystal violet stain, Mitotic figures, Oral epithelial dysplasia, Oral cancerAbstract
AIM: To evaluate mitotic activity in the different grades of oral epithelial dysplasia using 1% crystal violet stain.
MATERIAL AND METHODS: A descriptive study was conducted in the Department of Histopathology of the Post Graduate Medical Institute, Lahore on a total of thirty-three cases of the Oral Epithelial Dysplasia (OED). Fresh, frozen paraffin-embedded archival tissue blocks were collected from Lahore General Hospital, Lahore & Oral & Maxillofacial Surgery Department of Nawaz Sharif Hospital, Yakki Gate, Lahore. The representative sections were taken and, after processing, mounted on glass slides and stained with H&E and crystal violet stains. The stained slides were then examined under an optical microscope. The efficacy of 1% crystal violet stain to identify mitotic figures in the different grades of oral epithelial dysplasia was assessed with the sample t-test. A difference of p < 0.05 was considered to be significant.
RESULTS: A comparison of the mitotic figure count in two categories in sections stained with both stains showed a statistically significant difference. An increase in the mean mitotic count was noted in the sections of OED stained with crystal violet in comparison to the sections of OED stained with H&E which was statistically significant (p = 0.00).
CONCLUSION: Counting of mitotic cell is the rapid and simplest way of evaluating the proliferative activity of cells. Crystal violet stain can be a rationalised step in the staining of mitotic figures compared to the usual H&E staining and can be employed as a selective stain during routine histopathological procedures.
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Copyright (c) 2019 Aneequa Sajjad, Syeda Zaira Sajjad, Sadia Minhas, Muhammad Kashif, Afra Samad, Kanwar Ali Sajid, Munazza Hasan, Ihtesham-ud-Din Qureshi
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
http://creativecommons.org/licenses/by-nc/4.0