Study of Hemolysin Gene “aspHS” and Its Phenotype in Aspergillus Fumigatus

Abstract

AIM: The main goal of this study was to analysis the “aspHS†gene and its phenotype in A. fumigatus.

METHODS: Fifty-three A. fumigatus strains, including environmental, clinical and reference isolates, were used in this research. PCR was carried out based on Asp-hemolysin gene sequence. Two restriction enzymes TagI and NcoI were employed for digestion of PCR products.

RESULTS: PCR products of 180 and 450 bp were generated for all A. fumigatus isolates. Digestion of the aspHS gene 180 bp amplicons with TagI and 450 bp amplicons with TagI and NcoI produced the expected bands for most isolates. Hemolysin production of A. fumigatus isolates was evaluated on sheep blood agar (SBA).

CONCLUSION: In conclusion, our results provide evidence hemolysin activity and analysis of aspHS gene of A. fumigatus. These data may be useful in early diagnosis of A. fumigatus infections.