Hypoxia-Mesenchymal Stem Cells Inhibit Intra-Peritoneal Adhesions Formation by Upregulation of the IL-10 Expression

Authors

  • Adi Muradi Muhar Department of Doctoral Degree Program, Medical Faculty, Universitas Sumatera Utara, Medan, North Sumatera, Indonesia
  • Agung Putra Stem Cell And Cancer Research (SCCR), Medical Faculty, Sultan Agung Islamic University (UNISSULA), Semarang, Central Java, Indonesia; Department of Postgraduate Biomedical Science, Medical Faculty, Sultan Agung Islamic University (UNISSULA), Semarang, Central Java, Indonesia; Department of Pathological Anatomy, Medical Faculty, Sultan Agung Islamic University (UNISSULA), Semarang, Central Java, Indonesia http://orcid.org/0000-0003-4261-9437
  • Syah Mirsya Warli Department of Urology, Medical Faculty, Universitas Sumatera Utara, Medan, North Sumatera, Indonesia
  • Delfitri Munir Pusat Unggulan Inovasi (PUI) Stem Cell, Universitas Sumatera Utara (USU), Medan, North Sumatera, Indonesia

DOI:

https://doi.org/10.3889/oamjms.2019.713

Keywords:

Hypoxic, mesenchymal stem cell, IL-10, peritoneal adhesion

Abstract

BACKGROUND: Intra-peritoneal adhesions (IPAs) common occurre in post abdominal surgical. Athough many methods have been developed for controlling IPAs, including mesenchymal stem cells (MSCs) application, however, there is none completely preventing in due to the mesothelial structure may promote the prolonged inflammations leading. Nevertheless hypoxia-MSCs (H-MSCs) have more potent in controlling the inflammation than normoxia-MSCs (N-MSCs) by releasing several anti-inflamation particularly IL-10, however the H-MSCs application to inhibit IPAs remain unclear.

AIM: The aim of this study was to investigate the effectiveness of H-MSCs in preventing the AIPs event by releasing IL-10 on the ileum abrasion sutured omental patch as the animal model of peritoneal adhesion.

METHODS: Using 24 IPAs animal model were randomly divided into 4 groups: Sham (Sh), Control (C), H-MSCs at high dose (T1) and H-MSCs at low dose (T2). H-MSCs were incubated under hypoxic conditions (5% O2), 37°C and 5% CO2 for 24 hours. The expression level of IL-10 was performed using RT-PCR analysis. The macroscopic appearance of IPAs was evaluated using Nair’s scale base on the absence/presence of adhesion, whereas the microscopic by Zuhlke’s scale at Hematoxylin and eosin (H&E) staining.

RESULTS: This study showed a significanly increase in IL-10 expression (p < 0.05) at all T groups. In line with this, we also found a significant difference in IPAs between T groups and Control as well as a Sham (p < 0.05) either in the macroscopic or microscopic analysis.

CONCLUSION: H-MSCs has a robust ability in inhibiting severe IPAs characterized by the decreased of adhesion formation and the enhanced expression of IL-10.

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Published

2019-12-11

How to Cite

1.
Muhar AM, Putra A, Warli SM, Munir D. Hypoxia-Mesenchymal Stem Cells Inhibit Intra-Peritoneal Adhesions Formation by Upregulation of the IL-10 Expression. Open Access Maced J Med Sci [Internet]. 2019 Dec. 11 [cited 2024 Jul. 21];7(23):3937-43. Available from: https://oamjms.eu/index.php/mjms/article/view/oamjms.2019.713

Issue

Section

A - Basic Science

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