Phytochemical Profile and Pharmacological Activity of Vernonia amygdalina Delile Stem Bark Extracts Using Different Solvent Extraction

Muhammad Fauzan Lubis; Poppy Anjelisa Zaitun Hasibuan; Hafid Syahputra; Ririn Astyka; Intan Baruna

doi:10.3889/oamjms.2022.8921

Authors

Muhammad Fauzan Lubis Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia
Poppy Anjelisa Zaitun Hasibuan Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia
Hafid Syahputra Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia
Ririn Astyka Bachelor Program of Pharmacy, Faculty of Pharnacy, Universitas Sumatera Utara, Medan, Indonesia
Intan Baruna Phytochemical Laboratory, Departement of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia

DOI:

https://doi.org/10.3889/oamjms.2022.8921

Keywords:

Vernonia amygdalina, Phytochemicals, Antioxidant, Cytotoxic

Abstract

BACKGROUND: Vernonia amygdalina is a native plant from Africa which is widely distributed to Asia, especially in Indonesia. Parts of V. amygdalina such as roots, leaves, and bark are used by the community as traditional medicines such as antidiabetic, antibacterial, and anticancer.

AIM: This study aims to show the effect of solvents in the V. amygdalina stem bark extraction process on phytochemical’s content and their correlation with pharmacological activities.

METHODS: V. amygdalina extract from stem bark in this study was obtained using the maceration method with different solvents. The extracts were investigated for total phenolic content (TPC) and total flavonoids content (TFC) using calorimetry assay. Principal Component Analysis (PCA) was used to grouping the extracts based on Fourier-transform infrared (FTIR) data. Antioxidant activity of the extracts was done using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2’-azino-bis (3-ethyl benzhothiazoline sulphonic acid) (ABTS) assay. While, the cytotoxic activity of the extracts was carried out using MTT assay on PANC-1 cell line. The correlation phytochemical content and pharmacology activities of extracts were analyzed using person correlation method.

RESULTS: There were significantly different TPC and total flavonoid content of extracts (p < 0.05). The ethyl acetate extract was identified to have the highest TPC, TFC, DPPH, ABTS, and cytotoxic values of 3.61 ± 0.03 mg GAE/g dry powder, 25.12 ± 0.09 mg QE/g dry powder, 27.12 ± 0.65 μg/mL, 67.02 ± 0.23 μg/mL, and 33.83 ± 0.82 μg/mL, respectively (p < 0.05). This is supported by PCA analysis which shows that there are differences in extracts based on FTIR data and there is a strong correlation between TPC and TFC values with antioxidant and cytotoxic activities.

CONCLUSIONS: This study report that each extract of V. amygdalina stem bark gives a distinct phytochemical profile (TPC, TFC, and FTIR spectrum) that contributes to the antioxidant activity and cytotoxic activity.

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References

Yusoff SF, Haron FF, Muda Mohamed MT, Asib N, Sakimin SZ, Kassim F, et al. Antifungal activity and phytochemical screening of vernonia amygdalina extract against botrytis cinerea causing gray mold disease on tomato fruits. Biology (Basel). 2020;9(9):286. https://doi.org/10.3390/biology9090286 PMid:32932993 DOI: https://doi.org/10.3390/biology9090286

Erukainure OL, Chukwuma CI, Sanni O, Matsabisa MG, Islam MS. Histochemistry, phenolic content, antioxidant, and anti-diabetic activities of Vernonia amygdalina leaf extract. J Food Biochem. 2019;43(2):12737. https://doi.org/10.1111/jfbc.12737 PMid:31353661 DOI: https://doi.org/10.1111/jfbc.12737

Te Wang W, Liao SF, Wu ZL, Chang CW, Wu JY. Simultaneous study of antioxidant activity, DNA protection and anti-inflammatory effect of Vernonia amygdalina leaves extracts. PLoS One. 2020;15(7):0235717. https://doi.org/10.1371/journal.pone.0235717 PMid:32658905 DOI: https://doi.org/10.1371/journal.pone.0235717

Evbuomwan L, Chukwuka E, Obazenu E, Ilevbare L. All rights reserved antibacterial activity of Vernonia amygdalina leaf extracts against multidrug resistant bacterial isolates. J Appl Sci Environ Manag. 2018;22(1):17. https://doi.org/10.4314/jasem. v22i1.4 DOI: https://doi.org/10.4314/jasem.v22i1.4

Hasibuan PA, Harahap U, Sitorus P, Satria D. The anticancer activities of Vernonia amygdalina Delile. leaves on 4T1 breast cancer cells through phosphoinositide 3-kinase (PI3K) pathway. Heliyon. 2020;6(7):04449. https://doi.org/10.1016/j.heliyon.2020.e04449 DOI: https://doi.org/10.1016/j.heliyon.2020.e04449

Nguyen TX, Dang DL, Ngo VQ, Trinh TC, Trinh QN, Dong Do T, et al. Anti-inflammatory activity of a new compound from Vernonia amygdalina. Nat Prod Res. 2020;35(23):5160-5. https://doi.org/10.1080/14786419.2020.1788556 PMid:32633572 DOI: https://doi.org/10.1080/14786419.2020.1788556

Ajayi EI, Adeleke MA, Adewumi TY, Adeyemi AA. Antiplasmodial activities of ethanol extracts of Euphorbia hirta whole plant and Vernonia amygdalina leaves in plasmodium berghei-infected mice. J Taibah Univ Sci. 2017;11(6):831-5. https://doi.org/10.1016/j.jtusci.2017.01.008 DOI: https://doi.org/10.1016/j.jtusci.2017.01.008

Quasie O, Zhang YM, Zhang HJ, Luo J, Kong LY. Four new steroid saponins with highly oxidized side chains from the leaves of Vernonia amygdalina. Phytochem Lett. 2016;15:16-20. https://doi.org/10.1016/j.phytol.2015.11.002 DOI: https://doi.org/10.1016/j.phytol.2015.11.002

Eyong EU, Agiang MA, Atangwho IJ, Iwara IA, Odey MO, Ebong PE. Phytochemicals and micronutrients composition of root and stem bark extracts of Vernonia amygdalina Del. J Med Med Sci. 2011;2(6):900-3.

Ugbogu EA, Emmanuel O, Dike ED, Agi GO, Ugbogu OC, Ibe C, et al. The phytochemistry, ethnobotanical, and pharmacological potentials of the medicinal plant-Vernonia amygdalina L (bitter Leaf). Clin Complement Med Pharmacol. 2021;1(1):100006. https://doi.org/10.1016/j.ccmp.2021.100006 DOI: https://doi.org/10.1016/j.ccmp.2021.100006

Lu DY, Lu TR. Herbal medicine in new era. Hosp Palliat Med Int J. 2019;3(4):125-30. https://doi.org/10.15406/hpmij.2019.03.00165 DOI: https://doi.org/10.15406/hpmij.2019.03.00165

Wyrepkowski CC, Gomes da Costa DL, Paulo Sinhorin A, Vilegas W, De Grandis RA, Resende FA, et al. Characterization and quantification of the compounds of the ethanolic extract from Caesalpinia ferrea stem bark and evaluation of their mutagenic activity. Molecules. 2014;19(10):16039-57. https://doi.org/10.3390/molecules191016039 PMid:25299821 DOI: https://doi.org/10.3390/molecules191016039

Degu A, Kefale B, Alemayehu D, Tegegne G. Evaluation of the antidiarrheal activity of hydromethanol crude extracts of Ruta chalepensis and Vernonia amygdalina in Mice. Evid based Complement Altern Med. 2020;2020:8318713. https://doi.org/10.1155/2020/8318713 DOI: https://doi.org/10.1155/2020/8318713

Quartey AK, Forkuo-Donkor A, Ibira YJ, Ayensu I, Oduro-Kwarteng A, Noah P, et al. Hydroethanolic stem bark extract of Vernonia amygdalina Del. (Asteraceae) suppresses yeast-induced pyrexia and Plasmodium berghei malaria in murine models. J Med Plants Res. 2020;4(6):258-64. https://doi.org/10.5897/jmpr2020.6931 DOI: https://doi.org/10.5897/JMPR2020.6931

Chigayo K, Mojapelo PE, Mnyakeni-Moleele S, Misihairabgwi JM. Phytochemical and antioxidant properties of different solvent extracts of Kirkia wilmsii tubers. Asian Pac J Trop Biomed. 2016;6(12):1037-43. https://doi.org/10.1016/j.apjtb.2016.10.004 DOI: https://doi.org/10.1016/j.apjtb.2016.10.004

Rafi M, Meitary N, Septaningsih DA, Bintang M. Phytochemical profile and antioxidant activity of Guazuma ulmifolia leaves extracts using different solvent extraction. Indones J Pharm. 2020;31(3):171-80. https://doi.org/10.22146/ijp.598 DOI: https://doi.org/10.22146/ijp.598

Iloki-Assanga SB, Lewis-Luján LM, Lara-Espinoza CL, Gil-Salido AA, Fernandez-Angulo D, Rubio-Pino JL, et al. Solvent effects on phytochemical constituent profiles and antioxidant activities, using four different extraction formulations for analysis of Bucida buceras L. and Phoradendron californicum complementary and alternative medicine. BMC Res Notes. 2015;8(1):396. https://doi.org/10.1186/s13104-015-1388-1 PMid:26323940 DOI: https://doi.org/10.1186/s13104-015-1388-1

Dhanani T, Shah S, Gajbhiye NA, Kumar S. Effect of extraction methods on yield, phytochemical constituents and antioxidant activity of Withania somnifera. Arab J Chem. 2017;10(1):1193-9. https://doi.org/0.1016/j.arabjc.2013.02.015 DOI: https://doi.org/10.1016/j.arabjc.2013.02.015

Ng ZX, Koick YT, Yong PH. Comparative analyses on radical scavenging and cytotoxic activity of phenolic and flavonoid content from selected medicinal plants. Nat Prod Res. 2021;35(23):5271-6. https://doi.org/10.1080/14786419.2020.1749617 PMid:32290699 DOI: https://doi.org/10.1080/14786419.2020.1749617

Rosidah R, Yuandani Y, Widjaja SS, Auliafendri N, Muhammad M, Satria D, et al. Phytochemicals analysis and immunomodulatory activity of Saurauia vulcani korth. Leaves extracts towards raw 264.7 cell. Rasayan J Chem. 2021;14(2):1378-83. https://doi.org/10.31788/rjc.2021.1426075 DOI: https://doi.org/10.31788/RJC.2021.1426075

Asrin H, Hasibuan PA, Marianne M. Total phenolic content of ethanol extract of Artrocarpus camansi leave and its effect to superoxide dismutase (SOD) level in mice. Indones J Cancer Chemoprevention. 2018;8(3):101-109. https://doi.org/10.14499/indonesianjcanchemoprev8iss3pp101-109 DOI: https://doi.org/10.14499/indonesianjcanchemoprev8iss3pp101-109

Sinaga SM, Sudarmi S, Iksen I, Kevin K, Sari MP. Evaluation of total phenolic, flavonoid content, antioxidant and in vitro antilithogenesis activities of chives leaf (Allium schoenoprasum, L.). Rasayan J Chem. 2018;11(4):1604-8. https://doi.org/10.31788/RJC.2018.1144067 DOI: https://doi.org/10.31788/RJC.2018.1144067

Satria D, Silalahi J, Haro G, Ilyas S, Hsb PA. Antioxidant and antiproliferative activities of an ethylacetate fraction of picria fel-terrae lour. Herbs. Asian Pac J Cancer Prev. 2017;18(2):399-403. https://doi.org/10.22034/APJCP.2017.18.2.399 PMid:28345821

Johny LC, Kudre TG, Suresh PV. Production of egg white hydrolysate by digestion with pineapple bromelain: Optimization, evaluation and antioxidant activity study. J Food Sci Technol. 2021; 59:1-12. https://doi.org/10.1007/s13197-021-05188-0 PMid:34219806 DOI: https://doi.org/10.1007/s13197-021-05188-0

Hasibuan PA, Munir D, Pertiwi D, Satria D, Lubis MF. Flavonoids constituent analysis and cell cycle inhibition activity of ethylacetate extract of vernonia amygdalina delile. Leaves on lung cancer cell line. Rasayan J Chem. 2020;13(4):2577-81. https://doi.org/10.31788/RJC.2020.1345625 DOI: https://doi.org/10.31788/RJC.2020.1345625

Rafi M, Rismayani W, Sugiarti RM, Dyah Syafitri U, Wahyuni WT, Rohaeti E. FTIR-based fingerprinting combined with chemometrics for discrimination of Sonchus arvensis leaf extracts and correlation with their antioxidant activity. Indones J Pharm. 2021;32(2):132-40. https://doi.org/10.22146/ijp.755 DOI: https://doi.org/10.22146/ijp.755

Zhang Y, Huang Y, Zhang J, Zhang Y, Li Q, Li Y, et al. Ultrasonic extraction and oxidation characteristics of functional groups during coal spontaneous combustion. In: Fuel. Vol. 242. Netherlands: Elsvier; 2018. p. 287-94. https://doi.org/10.1016/j.fuel.2019.01.043 DOI: https://doi.org/10.1016/j.fuel.2019.01.043

Tran N, Pham B, Le L. Bioactive compounds in anti-diabetic plants: From herbal medicine to modern drug discovery. Biology(Basel). 2020;9(9):252. https://doi.org/10.3390/biology9090252 PMid:32872226 DOI: https://doi.org/10.3390/biology9090252

Azmir J, Ghafoor, Norulaini NA, Omar AK, Azmir J, Zaidul IS, et al. Techniques for extraction of bioactive compounds from plant materials: A review. J Food Eng. 2013;117(4):426-36. https://doi.org/10.1016/j.jfoodeng.2013.01.014 DOI: https://doi.org/10.1016/j.jfoodeng.2013.01.014

Hayouni EA, Abedrabba M, Bouix M, Hamdi M. The effects of solvents and extraction method on the phenolic contents and biological activities in vitro of Tunisian Quercus coccifera L. and Juniperus phoenicea L. fruit extracts. Food Chem. 2007;105(3):1126-34. https://doi.org/10.1016/j.foodchem.2007.02.010 DOI: https://doi.org/10.1016/j.foodchem.2007.02.010

Benayad Z, Martinez-Villaluenga C, Frias J, Gomez-Cordoves C, Es-Safi NE. Phenolic composition, antioxidant and anti-inflammatory activities of extracts from Moroccan Opuntia ficus-indica flowers obtained by different extraction methods. Ind Crops Prod. 2014;62:412-20. htps://doi.org/10.1016/j.indcrop.2014.08.046 DOI: https://doi.org/10.1016/j.indcrop.2014.08.046

Rafińska K, Pomastowski P, Rudnicka J, Krakowska A, Maruśka A, Narkute M, et al. Effect of solvent and extraction technique on composition and biological activity of Lepidium sativum extracts. Food Chem. 2019;289:16-25. https://doi.org/10.1016/j.foodchem.2019.03.025 PMid:30955598 DOI: https://doi.org/10.1016/j.foodchem.2019.03.025

Swamy MK, Arumugam G, Kaur R, Ghasemzadeh, Yusoff MM, Sinniah UR. GC-MS based metabolite profiling, antioxidant and antimicrobial properties of different solvent extracts of Malaysian Plectranthus amboinicus Leaves. Evid Based Complement Alternat Med. 2017;2017:1517683. https://doi.org/10.1155/2017/1517683 PMid:28424737 DOI: https://doi.org/10.1155/2017/1517683

Kaur S, Mondal P. Study of total phenolic and flavonoid content, antioxidant activity and antimicrobial properties of medicinal plants. J Microbiol Exp. 2014;1(1):23-8. DOI: https://doi.org/10.15406/jmen.2014.01.00005

Rosidah R, Hasibuan PA, Haro G, Masri P, Satria D. Antioxidant activity of alkaloid fractions of Zanthoxylum acanthopodium dc. Fruits with 1,1-diphenyl-2-picrylhydrazyl assay. Asian J Pharm Clin Res. 2018;11(13):33. https://doi.org/10.22159/ajpcr.2018.v11s1.26560 DOI: https://doi.org/10.22159/ajpcr.2018.v11s1.26560

Satria D, Silalahi J, Haro G, Ilyas S, Hasibuan PA. Chemical analysis and cytotoxic activity of N-hexane fraction of Zanthoxylum acanthopodium DC. Fruits. Rasayan J Chem. 2019;12(2):803-8. https://doi.org/10.31788/RJC.2019.1225180 DOI: https://doi.org/10.31788/RJC.2019.1225180

Hasibuan PA, Sitorus P, Satria D, Sibuea RD. Antioxidant properties and cytotoxic activity of ethyl acetate fraction of Plectranthus amboinicus (Lour) Spreng. Leaves on HeLa and T47D Cell Lines. Indones J Cancer Chemoprevention. 2019;10(1):37. https://doi.org/10.14499/indonesianjcanchemoprev10iss1pp37-45 DOI: https://doi.org/10.14499/indonesianjcanchemoprev10iss1pp37-45

Hassan LE, Khadeer Ahamed MB, Abdul Majid AS, Baharetha HM, Muslim NS, Nassar ZD, et al. Correlation of antiangiogenic, antioxidant and cytotoxic activities of some Sudanese medicinal plants with phenolic and flavonoid contents. BMC Complement Altern Med. 2014;14(1):406. https://doi.org/10.1186/1472-6882-14-406 PMid:25331269 DOI: https://doi.org/10.1186/1472-6882-14-406

Eddebbagh M, Massoudi NM, Abdelmjid A, Berrada M, Attaleb M, Benbacer L, et al. Correlation of the cytotoxic and antioxidant activities of moroccan pomegranate (Punica granatum) with phenolic and flavonoid contents. J Pharm Pharmacol. 2016; 4(9): 511-519. https://doi.org/10.17265/2328-2150/2016.09.006 DOI: https://doi.org/10.17265/2328-2150/2016.09.006

Jargalsaikhan G, Wu JY, Chen YC, Yang LL, Wu MS. Comparison of the phytochemical properties, antioxidant activity and cytotoxic effect on HepG2 cells in Mongolian and Taiwanese Rhubarb species. Molecules. 2021;26(5):1217. https://doi.org/10.3390/molecules26051217 PMid:33668690 DOI: https://doi.org/10.3390/molecules26051217