The PI3KCA and AKT Inhibitory Activities of Litsea Cubeba Lour. Fruits and Heartwoods Towards Hela Cells

Aminah Dalimunthe; Poppy Anjelisa Zaitun Hasibuan; Denny Satria

doi:10.3889/oamjms.2019.317

The PI3KCA and AKT Inhibitory Activities of Litsea Cubeba Lour. Fruits and Heartwoods Towards Hela Cells

Authors

Aminah Dalimunthe Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, 20155, Indonesia
Poppy Anjelisa Zaitun Hasibuan Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, 20155, Indonesia
Denny Satria Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, 20155, Indonesia

DOI:

https://doi.org/10.3889/oamjms.2019.317

Keywords:

Litsea cubeba Lour, Fruits, Heartwoods, PI3KCA, Akt

Abstract

AIM: To investigated the activities of chloroform fractions at pH 7 of Litsea cubeba Lour. Fruits and heartwoods (CF-7F and CF-7H) in decrease expression of PI3KCA, Akt-1 and Akt-2 genes towards cervical cancer cell culture (HeLa) experiments in vitro.

MATERIAL AND METHODS: CF-7F and CF-7H (12.5 and 25 Âµg/mL) were tested for its potential inhibition on gene expression of PI3KCA, Akt-1 and Akt-2 genes by Reverse Transcription-Polymerase Chain Reaction (RT-PCR) method.

RESULT: CF-7F and CF-7H were showed the activity to reduce the expression of PI3KCA, Akt-1 and Akt-2 genes.

CONCLUSION: Our results suggest that CF-7F and CF-7H significantly inhibit the expression of PI3KCA, Akt-1 and Akt-2 genes.

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Published

2019-05-14

How to Cite

Dalimunthe A, Hasibuan PAZ, Satria D. The PI3KCA and AKT Inhibitory Activities of Litsea Cubeba Lour. Fruits and Heartwoods Towards Hela Cells. Open Access Maced J Med Sci [Internet]. 2019 May 14 [cited 2023 Sep. 26];7(9):1422-4. Available from: https://oamjms.eu/index.php/mjms/article/view/oamjms.2019.317