Fasting Ghrelin Levels Are Decreased in Obese Subjects and Are Significantly Related With Insulin Resistance and Body Mass Index
Keywords:Ghrelin, obesity, Insulin resistance, glucose, Body mass Index
BACKGROUND: Ghrelin is a 28-amino acid peptide that predominantly produced by the stomach. Strong evidence indicates the effects of ghrelin in the regulation of metabolic functions and its potential role in the aetiology of obesity.
AIM: The aim of this study was to investigate the relationship of ghrelin levels with obesity, insulin resistance and glucose in normal and obese subjects.
METHODS: Thirteen normal (n = 13) and seven (n = 7) obese weight subjects aged 20-22 participated in the study. Fasting plasma ghrelin, insulin and glucose levels were measured after overnight fasting. HOMA-IR was calculated to evaluate insulin resistance.
RESULTS: Ghrelin and insulin levels were found to be statistically significantly lower and higher in obese subjects (P < 0.001), respectively. Glucose levels were clinically higher in obese subjects but not statistically significant. Fasting plasma ghrelin was negatively correlated with BMI (r = -0.77, P < 0.001), fasting insulin levels (r = -0.55, P < 0.001) and HOMA-IR (r = -0.66, P < 0.001). There was no correlation between ghrelin and glucose. In multiple regression analysis, insulin levels (Beta: -2.66, 95% CI: -2.49, -2.78, P < 0.001) HOMA-IR (Beta: -2.41, 95% CI: -2.33, -2.55, P < 0.001) and BMI (Beta: -1.77, 95% CI: -1.66, -1.89, P < 0.001) were significant independent determinants of fasting ghrelin.CONCLUSION: Obese subjects have low fasting ghrelin levels that they are significantly related to insulin resistance and body mass index. More prospective studies are needed to establish the role of ghrelin in the pathogenesis of human obesity.
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Ghelardoni S, Carnicelli V, Frascarelli S, et al. Ghrelin tissue distribution: comparison between gene and protein expression. J Endocrinol Invest. 2006; 29:115â€“121. https://doi.org/10.1007/BF03344083 PMid:16610236
Ukkola O. Ghrelin in Type 2 diabetes mellitus and metabolic syndrome. Mol Cell Endocrinol. 2011; 340:26â€“28. https://doi.org/10.1016/j.mce.2011.02.009 PMid:21419192
Boguszewski CL, Paz-Filho G, Velloso LA. Neuroendocrine body weight regulation: integration between fat tissue, gastrointestinal tract, and the brain. Endokrynol Pol. 2010; 61:194â€“206. PMid:20464707
TschÃ¶p M, Weyer C, Tataranni PA, et al. Circulating ghrelin levels are decreased in human obesity. Diabetes. 2001; 50:707â€“709. https://doi.org/10.2337/diabetes.50.4.707 PMid:11289032
SchÃ¶fl C, Horn R, Schill T, et al. Circulating ghrelin levels in patients with polycystic ovary syndrome. J Clin Endocrinol Metab. 2002; 87:4607â€“4610. https://doi.org/10.1210/jc.2002-020505 PMid:12364442
Ikezaki A, Hosoda H, Ito K, et al. Fasting plasma ghrelin levels are negatively correlated with insulin resistance and PAI-1, but not with leptin, in obese children and adolescents. Diabetes. 2002; 51:3408â€“3411. https://doi.org/10.2337/diabetes.51.12.3408 PMid:12453893
Marzullo P, Caumo A, Savia G, et al. Predictors of postabsorptive ghrelin secretion after intake of different macronutrients. J Clin Endocrinol Metab. 2006; 91: 4124â€“4130. https://doi.org/10.1210/jc.2006-0270 PMid:16882748
Moesgaard SG, Ahren B, Carr R, et al. Effects of high-fat feeding and fasting on ghrelin expression in the mouse stomach. Regul Pept. 2004; 120: 261â€“267. https://doi.org/10.1016/j.regpep.2004.03.018 PMid:15177945
Greenman Y, Golani N, Gilad S, et al. Ghrelin secretion is modulated in a nutrient- and gender-specific manner. Clin Endocrinol. 2004; 60: 382â€“388. https://doi.org/10.1111/j.1365-2265.2004.01993.x
Purnell JQ, Weigle DS, Breen P, et al. Ghrelin levels correlate with insulin levels, insulin resistance, and high-density lipoprotein cholesterol, but not with gender, menopausal status, or cortisol levels in humans. J Clin Endocrinol Metab. 2003; 88: 5747â€“5752. https://doi.org/10.1210/jc.2003-030513 PMid:14671163
Cole TJ, Bellizzi CM, Flegal MK, et al. Establishing a standard definition for child overweight and obesity worldwide: international survey. BMJ. 2000; 320: 1240-1243. https://doi.org/10.1136/bmj.320.7244.1240 PMid:10797032 PMCid:PMC27365
Matthews DR, Hosker JP, Rudenski AS, et al. Homeostasis model assessment: IR and beta-cell function from fasting plasma glucose and insulin concentration in man. Diabetologia. 1985; 28: 412-419. https://doi.org/10.1007/BF00280883 PMid:3899825
Lucidi P, Murdolo G, Di Loreto C, et al. Ghrelin is not necessary for adequate hormonal counterregulation of insulin-induced hypoglycemia. Diabetes. 2002; 51: 2911â€“2914. https://doi.org/10.2337/diabetes.51.10.2911 PMid:12351426
Flanagan DE, Evans ML, Monsod TP, et al. The influence of insulin on circulating ghrelin. American Journal of Physiology, Endocrinology and Metabolism. 2003; 284: E313â€“E316. https://doi.org/10.1152/ajpendo.00569.2001 PMid:12531744
Qader SS, Lundquist I, Ekelund M, et al. Ghrelin activates neuronal constitutive nitric oxide synthase in pancreatic islet cells while inhibiting insulin release and stimulating glucagon release. Regul Pept. 2005; 128: 51â€“56. https://doi.org/10.1016/j.regpep.2004.12.018 PMid:15721487
Soriano-GuillÃ©n L, Barrios V, Martos G, et al. Effect of oral glucose administration on ghrelin levels in obese children. Eur J Endocrinol. 2004; 151: 119â€“121. https://doi.org/10.1530/eje.0.1510119 PMid:15248831
Anderwald C, Brabant G, Bernroider E, et al. Insulin-dependent modulation of plasma ghrelin and leptin concentrations is less pronounced in type 2 diabetic patients. Diabetes. 2003; 52: 1792â€“1798. https://doi.org/10.2337/diabetes.52.7.1792 PMid:12829648
Covasa M, Swartz T. The role of ghrelin in eating behaviour. In: Preedy VR, Watson RR, Martin CR (eds), Handbook of behavior, food and nutrition. Springer Science, New York, 2011; 175-188. https://doi.org/10.1007/978-0-387-92271-3_13
Sato T, Ida T, Nakamura Y, et al. Physiological roles of ghrelin on obesity. Obes Res Clin Pract. 2014; 8: e405-413. https://doi.org/10.1016/j.orcp.2013.10.002 PMid:25263830
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