Pseudomonas aeruginosa - Modified Hodge Test (PAE-MHT) and ChromID Carba Agar for Detection of Carbapenemase Producing Pseudomonas Aeruginosa Recovered from Clinical Specimens

Authors

  • Hala B. Othman Clinical Pathology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt
  • Rania Mohamed Abdel Halim
  • Hoda Ezz El-arab Abdul-Wahab Clinical Pathology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt
  • Hossam Abol Atta Plastic and Reconstructive Surgery Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt
  • Omyma Shaaban Clinical Pathology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt

DOI:

https://doi.org/10.3889/oamjms.2018.414

Keywords:

CRPA, ChromID Carba Agar, MHT, PAE-MHT, PCR

Abstract

AIMS: This study aims to evaluate the ability of ChromID Carba agar, and Pseudomonas aeruginosa modified Hodge test (PAE-MHT) for detection of carbapenemase-producing P. aeruginosa and to determine the associated carbapenemase gene classes by PCR.

METHODS: One hundred Carbapenem-resistant P. aeruginosa (CRPA) isolates were tested for: i) carbapenemases production by ChromID carba agar, Modified Hodge test (MHT) and (PAE-MHT) and ii) detection of some carbapenemase genes by PCR.

RESULTS: All (100%) of the isolates showed growth on ChromID Carba agar with 100% sensitivity. Using MHT, 54% of isolates were positive, 3% were indeterminate, and 43% were negative, demonstrating 58.9% sensitivity and 80% specificity. On performing PAE-MHT, 91% of the strains were positive, 3% were intermediate, and 6% were negative, demonstrating 97.9% sensitivity and 80% specificity. The most prevalent gene was blaKPC (81%), followed by blaVIM (74%); blaIMP was detected in only one isolate, and blaOXA-48 in 34% of the isolates.

CONCLUSIONS: We conclude that PAE-MHT and ChromID Carba are sensitive, specific, simple and cost-effective screening tests for detection of CRPA isolates compared to the traditional MHT.

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References

Dortet L, Boulanger A, Poirel L, Nordmann P. Bloodstream infections caused by Pseudomonas spp.; how to detect carbapenemase producers directly from positive blood cultures?. Journal of clinical microbiology. 2014; JCM-03346.

Bert F, Branger C, Lambert-Zechovsky N. Identification of PSE and OXA β-lactamase genes in Pseudomonas aeruginosa using PCR–restriction fragment length polymorphism. Journal of Antimicrobial Chemotherapy. 2002; 50(1):11-8. https://doi.org/10.1093/jac/dkf069 PMid:12096001

Pasteran F, Veliz O, Faccone D, Guerriero L, Rapoport M, Mendez T, Corso A. A simple test for the detection of KPC and metalloâ€Î²â€lactamase carbapenemaseâ€producing Pseudomonas aeruginosa isolates with the use of meropenem disks supplemented with aminophenylboronic acid, dipicolinic acid and cloxacillin. Clinical Microbiology and Infection. 2011; 17(9):1438-41. https://doi.org/10.1111/j.1469-0691.2011.03585.x PMid:21689207

Simner PJ, Gilmour MW, DeGagne P, Nichol K, Karlowsky JA. Evaluation of five chromogenic agar media and the Rosco Rapid Carb screen kit for detection and confirmation of carbapenemase production in Gram-negative bacilli. Journal of clinical microbiology. 2015; 53(1):105-12. https://doi.org/10.1128/JCM.02068-14 PMid:25355764 PMCid:PMC4290907

Wayne PA. Clinical and laboratory standards institute. Performance standards for antimicrobial susceptibility testing. 2011:100-121.

Wayne P. Clinical and Laboratory Standards Institute (CLSI) performance standards for antimicrobial disk diffusion susceptibility tests 19th ed. approved standard. CLSI document M100-S19. 2009; 29(2011):M100- S21.

Pasteran F, Veliz O, Rapoport M, Guerriero L, Corso A. Sensitive and specific Modified Hodge Test for KPC and metallo-beta-lactamase detection in Pseudomonas aeruginosa by use of a novel indicator strain: Klebsiella pneumoniae ATCC 700603. Journal of clinical microbiology. 2011:JCM-05602. https://doi.org/10.1128/JCM.05602-11

Schechner V, Straus-Robinson K, Schwartz D, Pfeffer I, Tarabeia J, Moskovich R, Chmelnitsky I, Schwaber MJ, Carmeli Y, Navon-Venezia S. Evaluation of PCR-based testing for surveillance of KPC-producing carbapenem-resistant members of the Enterobacteriaceae family. Journal of clinical microbiology. 2009; 47(10):3261-5. https://doi.org/10.1128/JCM.02368-08 PMid:19675211 PMCid:PMC2756929

Poirel L, Nordmann P. Acquired carbapenem-hydrolyzing beta-lactamases and their genetic support. Current pharmaceutical biotechnology. 2002; 3(2):117-27. https://doi.org/10.2174/1389201023378427 PMid:12022255

Englen MD, Kelley LC. A rapid DNA isolation procedure for the identification of Campylobacter jejuni by the polymerase chain reaction. Letters in applied microbiology. 2000; 31(6):421-6. https://doi.org/10.1046/j.1365-2672.2000.00841.x

Gupte S, Kaur T. Clinical Importance of Carbapenemase Production in Gram-Negative Bacteria. Journal of Tropical Diseases & Public Health. 2015.

Noyal MJ, Menezes GA, Harish BN, Sujatha S, Parija SC. Simple screening tests for detection of carbapenemases in clinical isolates of nonfermentative Gram-negative bacteria. Indian Journal of Medical Research. 2009; 129(6):707. PMid:19692754

Pasteran F, Mendez T, Guerriero L, Rapoport M, Corso A. Sensitive screening tests for suspected class A carbapenemase production in species of Enterobacteriaceae. Journal of clinical microbiology. 2009; 47(6):1631-9. https://doi.org/10.1128/JCM.00130-09 PMid:19386850 PMCid:PMC2691115

Thomson KS. Extended-spectrum-β-lactamase, AmpC, and carbapenemase issues. Journal of clinical microbiology. 2010; 48(4):1019-25. https://doi.org/10.1128/JCM.00219-10 PMid:20181902 PMCid:PMC2849556

Girlich D, Poirel L, Nordamann P. Value of MHT for detection of emerging carbapenemasas in Enterobacteriacae. J Clin Microbiol. 2012; 50(2):477-479. https://doi.org/10.1128/JCM.05247-11 PMid:22116154 PMCid:PMC3264163

Roth AL, Kurpiel PM, Lister PD, Hanson ND. blaKPC RNA expression correlates with two transcriptional start sites but not always with gene copy number in four genera of Gram-negative pathogens. Antimicrobial agents and chemotherapy. 2011:AAC-01509.

Wayne PA. Clinical and laboratory standards institute. Performance standards for antimicrobial susceptibility testing, 2008.

Vrioni G, Daniil I, Voulgari E, Ranellou K, Koumaki V, Ghirardi S, Kimouli M, Zambardi G, Tsakris A. Comparative evaluation of a prototype chromogenic medium (ChromID CARBA) for detecting carbapenemase-producing Enterobacteriaceae in surveillance rectal swabs. Journal of clinical microbiology. 2012; JCM-06848. https://doi.org/10.1128/JCM.06848-11

Wilkinson KM, Winstanley TG, Lanyon C, Cummings SP, Raza MW, Perry JD. A Comparison of Four Chromogenic Culture Media for Carbapenemase-producing Enterobacteriaceae. Journal of clinical microbiology. 2012; JCM-01613. https://doi.org/10.1128/JCM.01613-12

Huang TD, Berhin C, Bogaerts P, Glupczynski Y. Comparative evaluation of two chromogenic tests for the rapid detection of carbapenemase in Enterobacteriaceae and in Pseudomonas aeruginosa isolates. Journal of clinical microbiology. 2014; JCM-00643. https://doi.org/10.1128/JCM.00643-14

Diene SM, Rolain JM. Carbapenemase genes and genetic platforms in Gramâ€negative bacilli: Enterobacteriaceae, Pseudomonas and Acinetobacter species. Clinical Microbiology and Infection. 2014; 20(9):831-8. https://doi.org/10.1111/1469-0691.12655 PMid:24766097

Carrër A, Fortineau N, Nordmann P. Use of ChromID extended-spectrum β-lactamase medium for detecting carbapenemase-producing Enterobacteriaceae. Journal of clinical microbiology. 2010; 48(5):1913-4. https://doi.org/10.1128/JCM.02277-09 PMid:20237104 PMCid:PMC2863866

Livermore DM, Woodford N. Carbapenemase: A problem in waiting? Curr Opin Microbiol. 2000; 3:489-95. https://doi.org/10.1016/S1369-5274(00)00128-4

Gupta V. Metallo beta lactamases in Pseudomonas aeruginosa and Acinetobacter species. Expert opinion on investigational drugs. 2008; 17(2):131-43. https://doi.org/10.1517/13543784.17.2.131 PMid:18230049

Poirel L, Rodríguez-Martínez JM, Al Naiemi N, Debets-Ossenkopp YJ, Nordmann P. Characterization of DIM-1, an integron-encoded metallo-β-lactamase from a Pseudomonas stutzeri clinical isolate in the Netherlands. Antimicrobial agents and chemotherapy. 2010; 54(6):2420-4.h

Jovcic B, Lepsanovic Z, Suljagic V, Rackov G, Begovic J, Topisirovic L, Kojic M. Emergence of NDM-1 metallo-β-lactamase in Pseudomonas aeruginosa clinical isolates from Serbia. Antimicrobial agents and chemotherapy. 2011; 55(8):3929-31. https://doi.org/10.1128/AAC.00226-11 PMid:21646490 PMCid:PMC3147624

Koutsogiannou M, Drougka E, Liakopoulos A, Jelastopulu E, Petinaki E, Anastassiou ED, Spiliopoulou I, Christofidou M. Spread of multidrug-resistant Pseudomonas aeruginosa clones in a university hospital. Journal of clinical microbiology. 2013; 51(2):665-8. https://doi.org/10.1128/JCM.03071-12 PMid:23241381 PMCid:PMC3553885

Samuelsen Ø, Toleman MA, Sundsfjord A, Rydberg J, Leegaard TM, Walder M, Lia A, Ranheim TE, Rajendra Y, Hermansen NO, Walsh TR. Molecular epidemiology of metallo-β-lactamase-producing Pseudomonas aeruginosa isolates from Norway and Sweden shows import of international clones and local clonal expansion. Antimicrobial agents and chemotherapy. 2010; 54(1):346-52. https://doi.org/10.1128/AAC.00824-09 PMid:19884381 PMCid:PMC2798561

Peshattiwar PD, Peerapur BV. ESBL and MBL mediated resistance in Pseudomonas aeruginosa: An emerging threat to clinical therapeutics. J Clin Diagn Res. 2011; 5(8):1552-4.

Upadhyay S, Sen MR, Bhattacharjee A. Presence of different beta-lactamase classes among clinical isolates of Pseudomonas aeruginosa expressing AmpC beta-lactamase enzyme. The Journal of Infection in Developing Countries. 2010; 4(04):239-42. PMid:20440062

Published

2018-11-25

How to Cite

1.
Othman HB, Abdel Halim RM, Abdul-Wahab HEE- arab, Atta HA, Shaaban O. Pseudomonas aeruginosa - Modified Hodge Test (PAE-MHT) and ChromID Carba Agar for Detection of Carbapenemase Producing Pseudomonas Aeruginosa Recovered from Clinical Specimens. Open Access Maced J Med Sci [Internet]. 2018 Nov. 25 [cited 2024 Mar. 29];6(12):2283-9. Available from: https://oamjms.eu/index.php/mjms/article/view/oamjms.2018.414

Issue

Section

A - Basic Science

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