Determination of Highest Dose of Ammonia without Effect at Work Environment through the Expression of Interleukin-2 Cell in Rattus Novergicus

Abdul Rohim Tualeka; Dwi Ananto Wibrata; Ahsan Ahsan; Pudji Rahmawati; Syamsiar S Russeng; Atjo Wahyu; Sukarmin Sukarmin

doi:10.3889/oamjms.2019.207

Authors

Abdul Rohim Tualeka Department of Occupational Health and Safety, Faculty of Public Health, Airlangga University, Surabaya, Indonesia
Dwi Ananto Wibrata Department of Nursing, Health Polytechnics of Ministry Health, Surabaya, Indonesia
Ahsan Ahsan Faculty of Nursing, University of Brawijaya, Malang, Indonesia
Pudji Rahmawati Department of Development of Islamic Society, State Islamic University Sunan Ampel, Surabaya, Indonesia
Syamsiar S Russeng Departement of Occupational Health and Safety, Faculty of Public Health, Hassanuddin University Indonesia, Makassar, Indonesia
Atjo Wahyu Departement of Occupational Health and Safety, Faculty of Public Health, Hassanuddin University Indonesia, Makassar, Indonesia
Sukarmin Sukarmin Department of Chemistry, State University of Surabaya, Surabaya, Indonesia

DOI:

https://doi.org/10.3889/oamjms.2019.207

Keywords:

Ammonia, Interleukin-2, NOAEL, White Mice, Remmele Scale Index

Abstract

BACKGROUND: For determining the threshold limit value firstly determined the highest dose of ammonia without effect (NOAEL). However, research on the determination of NOAEL ammonia didn't conduct in Indonesia.

AIM: The aim of this study to determine the value of the highest dose of ammonia without effect (No Observed Adverse Effect Level/NOAEL) through interleukin-2 (IL-2) expression on white mice.

METHODS: This study used experimental laboratory research with post-test only control group design using white mice as experimental subjects. The treatment group divided into 6 groups (a group of controls and five groups with different ammonia exposure through inhalation). The trend curve of Remmele Scale Index (IRS) and histopathologic analysis could be used for determining NOAEL.

RESULT: The location of the highest dose of ammonia without any effect (NOAEL) of white mice was in the second group, with 0.0103 mg/kg body weight dose. Analysis of statistical tests Kruskal Wallis stated there was no significant difference in interleukin-2 expression between the control with ammonia exposed group with a significance of p (0.747) > Î± (0.05).

CONCLUSION: There is no difference between some interleukin-2 expression in the lymphocyte cell lung white mice group exposed to ammonia and control group. The highest dose of ammonia without any effect (NOAEL) on white mice was 0.0103 mg/kg body weight.