The Effect of Ophiocephalus striatus sp. Extract on Nitric Oxide in Ischemic Stroke Model

Iskandar Nasution; Hasan Sjahrir; Syafruddin Ilyas; Muhammad Ichwan

doi:10.3889/oamjms.2021.6299

Authors

Iskandar Nasution Department of Neurology, Faculty of Medicine, University of Sumatera Utara, Haji Adam Malik General Hospital, Medan, Indonesia https://orcid.org/0000-0003-1342-0613
Hasan Sjahrir Department of Neurology, Faculty of Medicine, University of Sumatera Utara, Haji Adam Malik General Hospital, Medan, Indonesia
Syafruddin Ilyas Department of Biology, Faculty of Mathematics and Natural Science, University of Sumatera Utara, Medan, Indonesia
Muhammad Ichwan Department of Pharmacology and Therapeutics, Faculty of Medicine, University of Sumatera Utara, Haji Adam Malik General Hospital, Medan, Indonesia

DOI:

https://doi.org/10.3889/oamjms.2021.6299

Keywords:

Cerebral angiogenesis, Ophiocephalus striatus sp. extract, Nitric oxide, Ischemic stroke

Abstract

BACKGROUND: One of alternative medicine in stroke therapy is Ophiocephalus striatus sp. extract. The nutrients contained in the O. striatus sp. extract, namely amino acids, fatty acids, cuprum, and zinc, are useful for the process of angiogenesis in poststroke patients through increased endothelial nitric oxide synthase.

AIM: We hypothesized that there was an effect of giving O. striatus sp. extract to cerebral angiogenesis process of Sprague Dawley rats ischemic stroke models through the level of NO.

METHODS: This was evidenced by conducting experimental studies on rats ischemic stroke models which were divided into five groups, (a) K (−) group (no ligation, no treatment), (b) K (+) group (ligation, no treatment), (c) P1 group (ligation, 200 mg extract), (d) P2 group (ligation, 400 mg extract), and (e) P3 group (ligation, 800 mg extract). Then blood sample was taken on day 3 to assess levels of NO.

RESULTS: There was increased level of NO in P1 (p = 0.001), P2 (p < 0.001), and P3 (p < 0.001) groups compared to K (+) group. The level of NO increases along with the increasing dose of O. striatus sp. extract. Histological examination revealed that there was formation of new blood vessel in the P1, P2, and P3 groups compared to K (+) group.

CONCLUSION: Our study showed that O. striatus sp. extract improves cerebral angiogenesis in rat models of ischemic stroke.

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References

Feigin VL, Norrving B, Mensah GA. Global burden of stroke. Circ Res. 2017;120(3):439-48. https://doi.org/10.1161/circresaha.116.308413 PMid:28154096 DOI: https://doi.org/10.1161/CIRCRESAHA.116.308413

Hoeben A, Landuyt B, Highley MS, Wildiers H, Van Oosterom AT, De Bruijn EA. Vascular endotelial growth factor and angiogenesis. Pharmacol Rev. 2004;56(4):549-80. https://doi.org/10.1124/pr.56.4.3 PMid:15602010 DOI: https://doi.org/10.1124/pr.56.4.3

Oentaryo G, Istiati I and Soesilawati P. Acceleration of fibroblast number and FGF-2 expression using Channa striata extract induction during wound healing process: In vivo studies in wistar rats. Dent J (Majalah Kedokteran Gigi). 2016;49(3):125-32. https://doi.org/10.20473/j.djmkg.v49.i3.p125-132 DOI: https://doi.org/10.20473/j.djmkg.v49.i3.p125-132

Rahayu P, Marcelline F, Sulisttyaningrum E, Suhartono MT. Potential effect of striatin (DLBS0333), a bioactive protein fraction isolated from Channa striata for wound treatment. Asian Pasific J Trop Biomed. 2016;6(12):1001-7. https://doi.org/10.1016/j.apjtb.2016.10.008 DOI: https://doi.org/10.1016/j.apjtb.2016.10.008

Pudjonarko D, Abidin Z. Clinical outcome and arginine serum of acute ischemic stroke patients supplemented by Ophiocephalus striatus sp. extract. IOP Conf Ser Earth Environ Sci. 2018;116(1):012028. https://doi.org/10.1088/1755-1315/116/1/012028 DOI: https://doi.org/10.1088/1755-1315/116/1/012028

Indra MR, Gasmara CP. Metode UCAO (Unilateral serebral artery occlusion) meningkatkan kadar MMP-9 jaringan otak pada model tikus stroke iskemik. Malang Neurol J. 2016;2(2) :47-51. https://doi.org/10.21776/ub.mnj.2016.002.02.1 DOI: https://doi.org/10.21776/ub.mnj.2016.002.02.1

Gandin C, Widmann C, Lazdunski M, Heurteaux C. MLC901 favors angiogenesis and associated recovery after ischemic stroke in mice. Cerebrovasc Disc. 2016;42(1-2):139-54. https://doi.org/10.1159/000444810 PMid:27099921 DOI: https://doi.org/10.1159/000444810

Bederson JB, Pitts LH, Tsuji M, Nishimura MC, Davis RL, Bartkowski H. Rat middle serebral artery occlusion: Evaluation of the model and development of a neurologic examination. Stroke. 1986;17(3):472-6. https://doi.org/10.1161/01.str.17.3.472 PMid:3715945 DOI: https://doi.org/10.1161/01.STR.17.3.472

Siswanto A, Dewi N, Hayatie L. Effect of Haruan (Channa striata) extract on fibroblast cells count in wound healing. J Dentomaxillofac Sci. 2016;1(2):89-94. https://doi.org/10.15562/jdmfs.v1i2.3 DOI: https://doi.org/10.15562/jdmfs.v1i2.3

Norhalifah N, Rahmawanty D, Nurlely N. Uji efektivitas ekstrak air ikan haruan (Channa striata) asal Kalimantan selatan terhadap bleeding time dan clotting time secara in vivo. Med Farm. 2016;13(2):237-49. https://doi.org/10.24198/mfarmasetika.v4i0.25880 DOI: https://doi.org/10.24198/mfarmasetika.v4i0.25880

Nair AB, Jacob S. A simple practice guide for dose conversion between animals and human. J Basic Clin Pharm. 2016;7(2):27-31. https://doi.org/10.4103/0976-0105.177703 PMid:27057123 DOI: https://doi.org/10.4103/0976-0105.177703

Notoadmojo S. Metodologi Penelitian Kesehatan. Jakarta: PT Rineka Cipta; 2012.

Sahid NA, Hayati F, Rao CV, Ramely R, Sani I, Dzulkarnaen A, et al. Snakehead consumption enhances wound healing? From tradition to modern clinical practice: A prospective randomized controlled trial. Evid Based Complement Altern Med. 2018;2018:3032790. https://doi.org/10.1155/2018/3032790 DOI: https://doi.org/10.1155/2018/3032790

Chen J, Zacharek A, Zhang C, Jiang H, Li Y, Roberts C, et al. Endotelial nitric oxide synthase regulates brain-derived neurotrophic factor expression and neurogenesis after stroke in mice. J Neurosci. 2005;25(9):2366-75. https://doi.org/10.1523/jneurosci.5071-04.2005 PMid:15745963 DOI: https://doi.org/10.1523/JNEUROSCI.5071-04.2005

Chen K, Pittman RN, Popel AS. Nitric oxide in the vasculature: Where does it come from and where does it go? A quantitative perspective. Antioxid Redox Signal. 2008;10(7):1185-95. https://doi.org/10.1089/ars.2007.1959 PMid:18331202 DOI: https://doi.org/10.1089/ars.2007.1959

Carmeliet P, Collen D. Molecular basis of angiogenesis. Role of VEGF and VE-Cadherin. Ann N Y Acad Sci. 2000;902:249- 64; discussion 262-4. https://doi.org/10.1111/j.1749-6632.2000.tb06320.x PMid:10865845 DOI: https://doi.org/10.1111/j.1749-6632.2000.tb06320.x

Zhang R, Wang L, Zhang L, Chen J, Zhu Z, Zhang Z, et al. Nitric oxide enhances angiogenesis via the synthesis of vascular endothelial growth factor and cGMP after stroke in the rat. Circ Res. 2003;92(3):308-13. https://doi.org/10.1161/01.res.0000056757.93432.8c PMid:12595343 DOI: https://doi.org/10.1161/01.RES.0000056757.93432.8C

Dulak J, Jozkowicz A, Dembinska-Kiec A, Guevara I, Zdzienicka A, Zmudzinska-Grochot D, et al. Nitric oxide induces the synthesis of vascular endothelial growth factor by rat vascular smooth muscle cells. J Am Heart Assoc. 2013;20(3):659-66. https://doi.org/10.1161/01.atv.20.3.659 PMid:10712388 DOI: https://doi.org/10.1161/01.ATV.20.3.659

Li J, Perrella MA, Tsai JC, Yet SF, Hsieh CM, Yoshizumi M, et al. Induction of vascular endothelial growth factor gene expression by interleukin-1 in rat aortic smooth muscle cells. J Biol Chem. 1995;270(1):308-12. https://doi.org/10.1074/jbc.270.1.308 PMid:7814392 DOI: https://doi.org/10.1074/jbc.270.1.308

Ma Y, Qiang L, He M. Exercised therapy augments the ischemic-induced proangiogenic state and results in sustained improvement after stroke. Int J Mol Sci. 2013;14(4):8570-84. https://doi.org/10.3390/ijms14048570 PMid:23598418 DOI: https://doi.org/10.3390/ijms14048570

Tafani M, Sansone L, Limana F, Arcangeli T, De Santis E, Polese M, et al. The interplay of reactive oxygen species, hypoxia, inflammation and sirtuins in cancer initiation and progression. Oxid Med Cell Longev. 2016;2016:3907147. https://doi.org/10.1155/2016/3907147 PMid:26798421 DOI: https://doi.org/10.1155/2016/3907147

Armengou A, Hurtado O, Leira R, Obon M, Pascual C, Moro MA, et al. L-Arginine levels in blood as marker of nitric oxide-mediated brain damage in acute stroke: A clinical and experimental study. J Cereb Blood Flow Metab. 2003;23(8):978-84. https://doi.org/10.1097/01.wcb.0000080651.64357.c PMid:12902842 DOI: https://doi.org/10.1097/01.WCB.0000080651.64357.C6

Liu J, Wang Y, Akamatsu Y, Lee CC, Stetler RA, Lawton MT. Vascular remodeling after ischemic stroke: Mechanism and therapeutic potentials. Prog Neuobiol. 2014;115:138-56. https://doi.org/10.1016/j.pneurobio.2013.11.004 PMid:24291532 DOI: https://doi.org/10.1016/j.pneurobio.2013.11.004

Chopp M, Li Y, Chen J, Zhang R, Zhang Z. Brain repair and recovery from stroke. Eur Neurol. 2008;3(1):2-5. DOI: https://doi.org/10.17925/ENR.2008.03.01.47

Majewska I, Gendaszewska-Darmach E. Proangiogenic activity of plant extracts in accelerating wound healing a new face of old phytomedicines. 2011;58(4):449-60. https://doi.org/10.18388/abp.2011_2210 PMid:22030557 DOI: https://doi.org/10.18388/abp.2011_2210