Screening of Antimicrobial and Adhesive Activity of Lactobacilli Isolated from the National Food Products from Different Districts of the Karaganda Region (Kazakhstan)

Authors

  • Zhanerke Amirkhanova Department of Physiology, Buketov Karaganda University, Karaganda, Kazakhstan https://orcid.org/0000-0002-5487-3574
  • Saule Akhmetova Department of Biomedicine, Karaganda Medical University, Karaganda, Kazakhstan
  • Samat Kozhakhmetov Laboratory of Human Microbiome and Longevity “National Laboratory Astana”, Nazarbayev University, Nur-Sultan, Kazakhstan; Innovative Center ArtScience, Nur-Sultan, Kazakhstan https://orcid.org/0000-0001-9668-0327
  • Almagul Kushugulova Laboratory of Human Microbiome and Longevity “National Laboratory Astana”, Nazarbayev University, Nur-Sultan, Kazakhstan https://orcid.org/0000-0001-9479-0899
  • Rakhat Bodeeva Department of Physiology, Buketov Karaganda University, Karaganda, Kazakhstan
  • Zauresh Issina School of Dentistry, Karaganda Medical University, Karaganda, Kazakhstan
  • Marat Tusbayev School of Dentistry, Karaganda Medical University, Karaganda, Kazakhstan

DOI:

https://doi.org/10.3889/oamjms.2021.7053

Keywords:

Lactobacilli isolates, Antimicrobial activity, Adhesion, Traditional national lactic acid products, Matrix supported laser desorption/ionization flight time mass spectrometry

Abstract

BACKGROUND: It is a national priority to look for new probiotic bacteria with highly active biological properties to create a new generation of probiotics, ferments, therapeutic, and prophylactic fermented milk products, taking into account ethnocultural and regional characteristics.

AIM: The aim of the study is to assess probiotic properties of strains of lactobacilli (antimicrobial and adhesive), which are isolated from national lactic acid products from different districts of the Karaganda region (Kazakhstan).

MATERIALS AND METHODS: There were modern microbiological methods applied during the experiment. To determine the morpho-cultural properties, the following methods were used: Gram staining, a catalase test, serial dilutions. The Matrix Supported Laser Desorption/Ionization Flight Time Mass Spectrometry was used for identification, and the deferred-antagonism method was used to determine the antimicrobial activity. The buccal epithelial cells were used for the cell object as a test system to determine the adhesive activity.

RESULTS: In this experiment, 26 lactobacillus isolates were isolated from 68 samples of national lactic acid products produced in a traditional homemade way in different districts of the Karaganda region (Kazakhstan). As a result of the studies carried out on the cultural and morphological characteristics and identification by the mass spectrometer, the following lactobacilli were obtained: Lactobacillus acidophilus (two strains), Lactobacillus delbrueckii subsp. bulgaricum (two strains), Lactobacillus rhamnosus (seven strains), Lactobacillus plantarum (two strains), Lactobacillus paracasei (11 strains), and Lactobacillus fermentum (two strains). Twenty-six isolates of lactobacilli were tested for antimicrobial activity, 13 isolates of which showed an inhibitory effect, but the degree of antagonism varied among lactobacillus isolates. In general, the inhibitory activity of lactobacillus isolates was shown against the Gram-negative indicator microorganisms Salmonella typhimurium NCTC 12023, Escherichia coli NCTC 12923. The antibacterial activity was shown against the Staphylococcus aureus NCTC 12973 indicator microorganism in nine isolates of lactobacilli. Only six isolates of lactobacilli showed antifungal activity against the test strain of Candida albicans NCPF 3179. Out of 13 isolates of lactobacilli, nine isolates of medium and high activity competed for binding to buccal epithelial cells.

CONCLUSION: The obtained isolates from traditional dairy products are considered to be promising candidates and competitive isolates with some probiotic potential. This study calls for further researches to be made in this area.

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References

Aitzhanova AA, Oleinikova EA, Saubenova MG, Daugalieva ST, Berzhanova RZ. Selection of antagonistically active strains of lactic acid bacteria from milk of various animal species. Exp Biol. 2020;2(83):72-8. https://doi.org/10.26577//eb.2020.v83.i2.08 DOI: https://doi.org/10.26577//eb.2020.v83.i2.08

Kushugulova A, Kozhakhmetov S, Supiyev A, Shakhabayeva G, Saduakhasova S, Sabitkyzy S, et al. Isolation and characterization of lactobacilli from traditional Kazakh dairy products. Int J Probiotics. 2013;8(2-3):95-9.

Ghosh C, Sarkar P, Issa R, Haldar J. Alternatives of conventional antibiotics in the era of antimicrobial resistance. Trends Microbiol. 2019;27(4):323-38. https://doi.org/10.1016/j.tim.2018.12.010 PMid:30683453 DOI: https://doi.org/10.1016/j.tim.2018.12.010

Zeng Y, Li Y, Wu QP, Zhang JM, Xie XQ, Ding Y, et al. Evaluation of the antibacterial activity and probiotic potential of Lactobacillus plantarum Isolated from Chinese Homemade Pickles. Can J Infect Dis Med Microbiol. 2020;2020:8818989. https://doi.org/10.1155/2020/8818989 DOI: https://doi.org/10.1155/2020/8818989

Kushugulova A, Löber U, Akpanova S, Rysbekov K, Kozhakhmetov S, Khassenbekova Z, et al. Dynamic changes in microbiome composition following mare’s milk intake for prevention of collateral antibiotic effect. Front Cell Infect Microbiol. 2021;11:622735. https://doi.org/10.3389/fcimb.2021.622735 PMid:33968795 DOI: https://doi.org/10.3389/fcimb.2021.622735

Nemska V, Karsheva M, Danova S, Georgieva N. Microbiological, physico-chemical and rheological evaluation of yoghurts, fermented with lactobacilli from traditional Bulgarian dairy products. J Chem Technol Metal. 2021;56(3):526-32.

Wang D, Liu W, Ren Y, De L, Zhang D, Yang Y, et al. Isolation and identification of lactic acid bacteria from traditional dairy products in Baotou and Bayannur of Midwestern Inner Mongolia and q-PCR Analysis of Predominant. Korean J Food Sci Anim Resour. 2016;36(4):499-507. https://doi.org/10.5851/kosfa.2016.36.4.499 PMid:27621691 DOI: https://doi.org/10.5851/kosfa.2016.36.4.499

Alatoom AA, Cunningham SA, Ihde SM, Mandrekar JN, Patel R. Comparison of direct colony method versus extraction method for identification of gram-positive cocci by use of bruker biotyper matrix-assisted laser desorption ionization time of flight mass spectrometry J Clin Microbiol. 2011;49(8):2868-73. https://doi.org/10.1128/JCM.00506-11 PMid:21613431 DOI: https://doi.org/10.1128/JCM.00506-11

Anas M, Zinedine BA, Rizk HA, Eddine HJ, Mebrouk K. Screening of autochthonous Lactobacillus species from Algerian raw goats’ milk for the production of bacteriocin-like compounds against Staphylococcus aureus. Afr J Biotechnol. 2012;11(20):4595-607.

Nilova LY, Orishak EA, Boitsov AG. Regarding the problem of probiotics usage for intestinal disbacteriosis therapy. Sci J Prevent Clin Med. 2010;2(35):70-7.

Haukioja A, Yli-Knuuttila H, Loimaranta V, Kari K, Ouwehand AC, Meurman JH, et al. Oral adhesion and survival of probiotic and other lactobacilli and bifidobacteria in vitro. Oral Microbiol Immunol. 2006;21(5):326-32. https://doi.org/10.1111/j.1399-302X.2006.00299.x PMid:16922933 DOI: https://doi.org/10.1111/j.1399-302X.2006.00299.x

Russian Federation. General Pharmacopoeia Monograph (GPM).1.7.2.0012.15 Industrial Probiotic Strains and Strains for Probiotic Control; 2015. p. 49.

Kabanikhin S, Krivorotko O, Takuadina A, Andornaya D, Zhang S. Geo-information system of tuberculosis spread based on inversion and prediction. J Inver III Posed Probl. 2021;29(1):65-79. https://doi.org/10.1515/jiip-2020-0022 DOI: https://doi.org/10.1515/jiip-2020-0022

Kintonova A, Ye N, Arynova Z, Kussepova L, Karymsakova A, Takuadina A, et al. Automation of business processes at the enterprise during a brand formation. Ad Alta. 2019;9(1):107-13.

Zheng J, Wittouck S, Salvetti E, Franz CM, Harris HMB, Mattarelli P, et al. A taxonomic note on the genus Lactobacillus: Description of 23 novel genera, emended description of the genus Lactobacillus Beijerinck 1901, and union of Lactobacillaceae and Leuconostocaceae. Int J Syst Evol Microbiol. 2020;70(2):2782-858. https://doi.org/10.1099/ijsem.0.004107 PMid:32293557 DOI: https://doi.org/10.1099/ijsem.0.004107

Kanak EK, Yilmaz SÖ. Maldi-tof mass spectrometry for the identification and detection of antimicrobial activity of lactic acid bacteria isolated from local cheeses. J Food Sci Technol. 2019;39(2):462-69. https://doi.org/10.1590/fst.19418 DOI: https://doi.org/10.1590/fst.19418

Pavlovic M, Huber I, Konrad R, Busch U. Application of MALDI-TOF MS for the identification of food borne bacteria. Open Microbiol J. 2013;7(1):135-41. https://doi.org/10.2174/1874285801307010135 PMid:24358065 DOI: https://doi.org/10.2174/1874285801307010135

Coman MM, Verdenelli MC, Cecchini C, Silvi S, Orpianesi C, Boyko N, et al. In vitro evaluation of antimicrobial activity of Lactobacillus rhamnosus IMC 501(®), Lactobacillus paracasei IMC 502(®) and SYNBIO(®) against pathogens. J Appl Microbiol. 2014;117(2):518-27. https://doi.org/10.1111/jam.12544 PMid:24836638 DOI: https://doi.org/10.1111/jam.12544

Miladi H, Zmantar T, Chaabouni Y, Fedhila K, Bakhrouf A, Mandouani K, et al. Antibacterial and efflux pump inhibitors of thymol and carvacrol against food-borne pathogens. Microb Pathog. 2016;99:95-100. https://doi.org/10.1016/j.micpath.2016.08.008 PMid:27521228 DOI: https://doi.org/10.1016/j.micpath.2016.08.008

Kumar M, Dhaka P, Vijay D, Vergis J, Mohan V, Kumar A, et al. Antimicrobial effects of Lactobacillus plantarum and Lactobacillus acidophilus against multidrug-resistant enteroaggregative Escherichia coli. Int J Antimicrob Agents. 2016;48(3):265-70. https://doi.org/10.1016/j.ijantimicag.2016.05.014 PMid:27451088 DOI: https://doi.org/10.1016/j.ijantimicag.2016.05.014

Kang MS, Lim HS, Oh JS, Lim YJ, Wuertz-Kozak K, Harro JM, et al. Antimicrobial activity of Lactobacillus salivarius and Lactobacillus fermentum against Staphylococcus aureus. Pathog Dis. 2017;75(2):1-9. https://doi.org/10.1093/femspd/ftx009 PMid:28158586 DOI: https://doi.org/10.1093/femspd/ftx009

Muhammad Z, Ramzan R, Abdelazez A, Amjad A, Afzaal M, Zhang S, et al. Assessment of the Antimicrobial Potentiality and Functionality of Lactobacillus plantarum Strains Isolated from the Conventional Inner Mongolian Fermented Cheese Against Foodborne Pathogens» Pathogens. 2019; 8(2):71. https://doi.org/10.3390%2Fpathogens8020071 DOI: https://doi.org/10.3390/pathogens8020071

Silvaa DR, Sardib JC, Pitanguic NS, Roquee SM, Silva AC, Rosalenad PL. Probiotics as an alternative antimicrobial therapy: Current reality and future directions. J Funct Foods. 2020;73:104080. https://doi.org/10.1016/j.jff.2020.104080 DOI: https://doi.org/10.1016/j.jff.2020.104080

Kivanç M, Yilmaz M, Çakir E. Isolation and identification of lactic acid bacteria from boza, and their microbial activity against several reporter strains. Turk J Biol. 2011;35(3):313-24. https://doi.org/10.3906/biy-0906-67

Duary RK, Rajput YS, Batish VK, Grover S. Assessing the adhesion of putative indigenous probiotic lactobacilli to human colonic epithelial cells. Ind J Med Res. 2011;134(5):664-71. https://doi.org/10.4103/0971-5916.90992 PMid:22199106 DOI: https://doi.org/10.4103/0971-5916.90992

Collado MC, Surono I, Meriluoto J, Salminen S. Indigenous dadih lactic acid bacteria: cell-surface properties and interactions with pathogens. J Food Sci. 2007;72(3):М89-93. https://doi.org/10.1111/j.1750-3841.2007.00294.x PMid:17995806 DOI: https://doi.org/10.1111/j.1750-3841.2007.00294.x

Stoianova LG, Ustiugova E, Netrusov AI. Antibacterial metabolites of lactic acid bacteria: Their diversity and properties. Prikl Biokhim Mikrobiol. 2012;48(3):259-75. PMid:22834296 DOI: https://doi.org/10.1134/S0003683812030143

Prabhurajeshwar C, Chandrakanth RK. Probiotic potential of lactobacilli with antagonistic activity against pathogenic strains: An in vitro validation for the production of inhibitory substances. Biomed J 2017;40(5):270-83. https://doi.org/10.1016/j.bj.2017.06.008 PMid:29179882 DOI: https://doi.org/10.1016/j.bj.2017.06.008

Jose NM, Bunt CR, McDowell A, Chiu JZ, Hussain MA. Short communication: A study of Lactobacillus isolates’ adherence to and influence on membrane integrity of human Caco-2 cells. J. Dairy Sci. 2017;100(10):7891-96. https://doi.org/10.3168/jds.2017-12912 DOI: https://doi.org/10.3168/jds.2017-12912

Lebeer S, Claes I, Tytgat HL, Verhoeven TL, Marien E, von Ossowski I, et al. Functional analysis of Lactobacillus rhamnosus GG pili in relation to adhesion and immunomodulatory interactions with intestinal epithelial cells. Appl Environ Microbiol. 2012;78(1):185-93. https://doi.org/10.1128%2FAEM.06192-11 PMid:22020518 DOI: https://doi.org/10.1128/AEM.06192-11

Tuo Y, Yu H, Ai L, Wu Z, Guo B, Chen W. Aggregation and adhesion properties of 22 Lactobacillus strains. J Dairy Sci. 2013;96(7):4252-7. https://doi.org/10.3168/jds.2013-6547 PMid:23664349 DOI: https://doi.org/10.3168/jds.2013-6547

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Published

2021-09-24

How to Cite

1.
Amirkhanova Z, Akhmetova S, Kozhakhmetov S, Kushugulova A, Bodeeva R, Issina Z, Tusbayev M. Screening of Antimicrobial and Adhesive Activity of Lactobacilli Isolated from the National Food Products from Different Districts of the Karaganda Region (Kazakhstan). Open Access Maced J Med Sci [Internet]. 2021 Sep. 24 [cited 2024 Mar. 28];9(A):827-32. Available from: https://oamjms.eu/index.php/mjms/article/view/7053

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