Tissue-Cultured Human Cord Lining Epithelial Cells in Treatment of Persistent Corneal Epithelial Defect

Nguyen Dinh  Ngan; Hoang Minh  Chau; Pham Ngoc  Dong; Le Xuan  Cung; Nguyen Thu  Thuy; Phan Toan  Thang; Than Van  Thai; Vu Thi  Nga; Nguyen Duy Bac

doi:10.3889/oamjms.2019.372

Authors

Nguyen Dinh Ngan Department of Ophthalmology, 103 Military Hospital, Hanoi, Vietnam; Department of Ophthalmology, Vietnam Military Medical University, Hanoi, Vietnam
Hoang Minh Chau Department of Cornea and external diseases, Vietnam National Institute of Ophthalmology, Hanoi, Vietnam; Department of Ophthalmology, Hanoi Medical University, Department of Ophthalmology
Pham Ngoc Dong Department of Cornea and external diseases, Vietnam National Institute of Ophthalmology, Hanoi, Vietnam
Le Xuan Cung Department of Cornea and external diseases, Vietnam National Institute of Ophthalmology, Hanoi, Vietnam
Nguyen Thu Thuy Department of Cornea and external diseases, Vietnam National Institute of Ophthalmology, Hanoi, Vietnam
Phan Toan Thang Department of Surgery, Yong Loo Lin School of Medicine, National University of Singapore, Singapore
Than Van Thai NTT Hi-tech Institute, Nguyen Tat Thanh University, Ho Chi Minh City, Vietnam
Vu Thi Nga Institute for Research and Development, Duy Tan University, 03 Quang Trung, Danang, Vietnam
Nguyen Duy Bac Vietnam Military Medical University (VMMU), Hanoi, Vietnam

DOI:

https://doi.org/10.3889/oamjms.2019.372

Keywords:

Persistent corneal epithelial defect, Cord lining epithelial cells, Tissue-cultured epithelial cells

Abstract

BACKGROUND: Persistent corneal epithelial defect (PED) is a consequence of many ocular surface disorders. Although many therapies have been suggested, the treatment of this disease have faced a lot of difficulties up to now. The transplatation of cultivated amniotic epithelial cells sheets is the new promised method for PED. Cord lining epithelial cells (CLECs) are epithelial cells of amniotic membrane of umbilical cord, so these cultivated cells sheet may be good for treating PED

AIM: To evaluate the efficacy of the transplantation of cultivated CLECs sheets in treatment of PED and analyze some influential factors of this therapy.

METHODS: A prospective interventional case series with transplantation of tissue-cultured human CLECs in 37 PED eyes in Vietnam National Institute of Ophthalmology.

RESULTS: Thirty four of 37 eyes were healed with the cells transplantation and 22 eyes of them healed within a week postoperatively. There were normal corneal scars and normal corneal epithelial cell (by impression cytology detection) on transplantation site in all 31 successful cases. The other successful eyes were done lamellar keratoplasty (respectively in 1 month, 3 months, 6 months and 27 months postoperatively) to investigate the histopathology of the CLECs transplant site. The histopathological images showed normal corneal scar and there was no appearance of CLECs in transplant site.

CONCLUSION: tissue-cultured human CLECs transplantation is a quite safe and effective treatment for persistent corneal epithelial defect. The CLECs may help the epithelial healing at early stage but do not exist at transplant site for a long time.

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