Transformation Chlorophyll a of Spirulina platensis to Chlorin e6 Derivatives and Several Applications

Hoa Thi Hai  Bui; Tam  Thi Pham; Hien  Thi Thu Nguyen; Trung  Minh Do; Vu  Thi Nga; Nguyen Duy Bac; Vu Thi Bich Huyen; Hai Minh Le; Quang Canh Tran

doi:10.3889/oamjms.2019.838

Authors

Hoa Thi Hai Bui Hanoi Open University, Hanoi, Vietnam
Tam Thi Pham Hanoi Open University, Hanoi, Vietnam
Hien Thi Thu Nguyen Hanoi Open University, Hanoi, Vietnam
Trung Minh Do Vietnam Military Medical University, Hanoi, Vietnam
Vu Thi Nga Institute for Research and Development, Duy Tan University, 03 Quang Trung, Danang, Vietnam
Nguyen Duy Bac Vietnam Military Medical University, Hanoi, Vietnam
Vu Thi Bich Huyen Department of Genetics and Biochemistry, Faculty of Biology, Hanoi National University of Education
Hai Minh Le Vinh University, Nghe An, Vietnam
Quang Canh Tran Center for Hygiene and Food Safety, Haiduong Medical Technical University, Hai Duong, Vietnam

DOI:

https://doi.org/10.3889/oamjms.2019.838

Keywords:

Spirulina platensis, Chlorin e6 trimethylester, Photodynamic therapy, Hela cell, Antibacterial

Abstract

BACKGROUND: Spirulina platensis contains a large amount of chlorophylls, chlorophyll a, that are starting materials to synthesize functionalized chlorins. Chlorin e6 (Ce6) as well as its derivatives are second generation sensitizers using in photodynamic therapy (PDT) of various cancers. In this study, we transfer chlorophyll a of S. platensis to Ce6derivatives and determine their several applications.

AIM: to evaluate the effects of Ce6 derivatives to treat cancer cells.

METHODS: Ce6 trimethylester was created from methyl pheophorbide a2 in S. platensis provided by the Hidumi Company, Nghe An province, Viet Nam. Hela cells were incubated with Ce6 trimethylester and the irradiated with the diode laser dose of 1.2 J/cm²/min through the system of filters Â£ 650 nm. MTT assay and clonogenic assay were used to determine survival rate and cloning efficiency of cells. Antimicrobial effect of Ce6 trimethylester with halogen light were studied with Propionibacterium acnes VTCC 0218 and Staphylococcus aureus VTCC 0173.

RESULTS: From dry biomass (700 g) of S. platensis, after extracting chlorophyll a and methanolysis, 4.2 g of methyl pheophorbide a was obtained. The reaction to give Ce6 trimethylester with 82% yield was performed with potassium hydroxide (KOH) in MeOH/THF/CHCl3. After irradiation with a 650 nm laser at 1.2 J, the cell viability in all samples decreased with Ce6 trimethylester treatment, the survival declining trend of Hela cells treated with Ce6 trimethylester were proportional when concentration of Ce6 trimethylester increased. The rate of colony formation was declined as the concentration of Ce6 trimethylester treated was increased. The growth of both S. aureus and P. acnes can be inactivated by Ce6 trimethylester PDT. The MIC99 value against P. acnes VTCC 0218 and S. aureus VTCC 0173 of Ce6 trimethylester with halogen light was 1.25 Î¼g/ml.

CONCLUSION: The Ce6 trimethylester from S. platensis cultivated in Viet Nam could be used as a potential photosentizer for photodynamic therapy for treatment of cancer and acne.