Antibiotic Resistance Profile and Diversity of Subtypes Genes in Escherichia coli Causing Bloodstream Infection in Northern Vietnam

Pham Ngoc  Hung; Do  Quyet; Kieu Chi  Thanh; Dinh Cong  Pho; Tran Viet  Tien; Quan Anh  Dung; Do Dieu  Linh; Ha The  Tan; Thien  Chu Dinh; Nguyen  Duy Bac; Le Van Nam

doi:10.3889/oamjms.2019.842

Authors

Pham Ngoc Hung Department of Epidemiology, Vietnam Military Medical University, Hanoi, Vietnam; Department of Training, Vietnam Military Medical University, Hanoi, Vietnam
Do Quyet Department of Tuberculosis and Lung Diseases, Military Hospital 103, Vietnam Military Medical University, Hanoi, Vietnam
Kieu Chi Thanh Department of Hospital Infection Control, Military Hospital 103, Vietnam Military Medical University, Hanoi, Vietnam
Dinh Cong Pho Faculty of Medicine, Vietnam Military Medical University, Hanoi, Vietnam
Tran Viet Tien Department of Infectious Diseases, Military Hospital 103, Vietnam Military Medical University, Hanoi, Vietnam
Quan Anh Dung Faculty of Medicine, Vietnam Military Medical University, Hanoi, Vietnam
Do Dieu Linh Faculty of Medicine, Hai Phong Medical University, 72A Nguyen Binh Khiem, Hai Phong, Vietnam
Ha The Tan Department of Epidemiology, Vietnam Military Medical University, Hanoi, Vietnam
Thien Chu Dinh Institute for Research and Development, Duy Tan University, 03 Quang Trung, Danang, Vietnam
Nguyen Duy Bac Department of Training, Vietnam Military Medical University, Hanoi, Vietnam
Le Van Nam Department of Infectious Diseases, Military Hospital 103, Vietnam Military Medical University, Hanoi, Vietnam

DOI:

https://doi.org/10.3889/oamjms.2019.842

Keywords:

Antibiotic resistance, Escherichia coli (E. coli), ESBL-producing, BSIs (bloodstream infections)

Abstract

BACKGROUND: Evaluating the antibiotic susceptibility and resistance genes is essential in the clinical management of bloodstream infections (BSIs). But there are still limited studies in Northern Vietnam.

AIM: The aim of the study was to determine the antibiotic resistance profile and characteristics of subtypes genes in Escherichia coli causing BSIs in Northern Vietnam.

METHODS: The cross-sectional study was done in the period from December 2012 to June 2014 in two tertiary hospitals in Northern Vietnam. Tests were performed at the lab of the hospital.

RESULTS: In 56 E. coli strains isolating 39.29 % produced ESBL. 100% of the isolates harbored blaTEM gene, but none of them had the blaPER gene. The prevalence of ESBL producers and ESBL non-producers in blaCTX-M gene was 81.82%, and 73.53%, in blaSHV gene was 18.18% and 35.29%. Sequencing results showed three blaTEM subtypes (blaTEM 1, 79, 82), four blaCTX-M subtypes (blaCTX-M-15, 73, 98, 161), and eight blaSHV subtypes (blaSHV 5, 7, 12, 15, 24, 33, 57, 77). Antibiotic resistance was higher in ampicillin (85.71%), trimethoprim/sulfamethoxazole (64.29%) and cephazolin (50%). Antibiotics were still highly susceptible including doripenem (96.43%), ertapenem (94.64%), amikacin (96.43%), and cefepime (89.29%).

CONCLUSION: In Escherichia coli causing BSIs, antibiotic resistance was higher in ampicillin, trimethoprim/sulfamethoxazole and cephazolin. Antibiotics was highly susceptible including doripenem, ertapenem, amikacin, and cefepime.