Comparison of Serum IgG Antibody Test with Gastric Biopsy for the Detection of Helicobacter Pylori Infection among Egyptian Children
DOI:
https://doi.org/10.3889/oamjms.2015.062Keywords:
Helicobacter pylori, serologic test, children, EgyptAbstract
BACKGROUND: In developing countries, Helicobacter pylori (H. pylori) infection is mainly acquired during childhood and may be a predisposing factor for peptic ulcer or gastric cancer later in life. Noninvasive diagnostic tools are particularly useful in children for screening tests and epidemiological studies. Data on serologic testing of children are lacking. Accurate noninvasive tests for diagnosing Helicobacter pylori infection in children are strongly required.
AIM: The aim of this study was to evaluate the performance of a serological test (serum IgG antibody for H. pylori) in Egyptian children with recurrent abdominal pain necessitating endoscopy.
SUBJECTS AND METHODS: One hundred children, referred to the endoscopy unit at Mansoura University. Upper endoscopy was done for each with rapid urease test (RUT) and histological examination as the gold standard test for detection of H. pylori infection. Serum samples were collected for detecting IgG for H. pylori infection.
RESULTS: The mean age of the subjects included in the study was 7.23 ± 1.94 year. Serological test (IgG to H. pylori) was positive in 60% of all cases. A highly significant association between the standard test and the serological test at a cutoff > 10 U/ml at p = 0.001 were detected for the diagnosis of H. pylori infection. The sensitivity, specificity, positive likelihood ratio, and negative likelihood ratio for the IgG antibody a cutoff > 10 U/ml, were 96.5%, 93%, 13.83, 0.038 respectively.
CONCLUSION: Serum IgG antibody to H. pylori infection has a high diagnostic value and can be considered as a suitable and reliable noninvasive test for detection of H. pylori infection.Downloads
Metrics
Plum Analytics Artifact Widget Block
References
Elfant AB. Helicobacter pylori: History, Prevalence, and Association with disease. Gastroenterology & Hepatology. 2012; 8(11) Supplement 7: 3-6.
Mansour MMHK, Al Hadidi KH M, Omar MA. Helicobacter pylori and recurrent abdominal pain in children: Is there any relation? Tropical Gastroenterology. 2012; 33(1): 55–61. DOI: https://doi.org/10.7869/tg.2012.9
Hamed ME, Hussein HM, El Sadany HF, Elgobashy AA, Atta AH. Seroprevalence of Helicobacter pylori infection among family members of infected and non-infected symptomatic children. J Egypt Soc Parasitol. 2013; 43(3): 755-66. DOI: https://doi.org/10.21608/jesp.2013.93322
Prasanthi CH, Prasanthi NL, Manikiran SS, Rama Rao NN. Focus on current trends in the treatment of Helicobacter pylori infection: An update. Inter J Pharm Sci Rev Res. 2011; 1: 42-51.
McColl KE. Clinical practice. Helicobacter pylori infection. N Engl J Med. 2010; 362: 1597–604. DOI: https://doi.org/10.1056/NEJMcp1001110
Tan HJ, Goh KL. Extra gastrointestinal manifestations of Helicobacter pylori infection: Facts or myth? A critical review. J Dig Dis. 2012; 13: 342–9. DOI: https://doi.org/10.1111/j.1751-2980.2012.00599.x
Zhao Y, Wang J, Tanaka T, Hosono A, Ando R, Soeripto S, Ediati Triningsih FX, Triono T, Sumoharjo S, Astuti EY, Gunawan S, Tokudome S. Association between HLA-DQ genotypes and haplotypes vs. Helicobacter pylori infection in an Indonesian population. Asian Pac J Cancer Prev. 2012; 13: 1247-51. DOI: https://doi.org/10.7314/APJCP.2012.13.4.1247
Lopes AI, Vale FF, Oleastro M. Helicobacter pylori infection - recent developments in diagnosis. World J Gastroenterol. 2014; 20(28): 9299-9313.
Guarner J, Kalach N, Elitsur Y, Koletzko S. Helicobacter pylori diagnostic tests in children: Review of the literature from 1999 to 2009. Eur J Pediatr. 2010; 169: 15-25. DOI: https://doi.org/10.1007/s00431-009-1033-x
Sabbi T, De Angelis P, Colistro F, Dall’Oglio L, di Abriola GF, Castro M. Efficacy of noninvasive tests in the diagnosis of Helicobacter pylori infection in pediatric patients. Arch Pediatr Adolesc Med. 2005; 159: 238. DOI: https://doi.org/10.1001/archpedi.159.3.238
Valliani A, Khan F, Chagani B,Khuwaja AK, Majid S, Hashmi S, Nanji K, Valliani S.. Factors associated with Helicobacter pylori infection, results from a developing country - Pakistan. Asian Pac J Cancer Prev. 2013; 14: 53-6. DOI: https://doi.org/10.7314/APJCP.2013.14.1.53
Burucoa C, Delchier JC, Courillon-Mallet A, de Korwin JD, Mégraud F, Zerbib F, Raymond J, Fauchère JL. Comparative evaluation of 29 commercial Helicobacter pylori serological kits. Helicobacter. 2013; 18: 169-179. DOI: https://doi.org/10.1111/hel.12030
Choi J, Kim CH, Kim D, Chung SJ, Song JH, Kang JM, Yang JI, Park MJ, Kim YS, Yim JY, Lim SH, Kim JS, Jung HC, Song IS. Prospective evaluation of a new stool antigen test for the detection of Helicobacter pylori, in comparison with histology, rapid urease test, (13)C-urea breath test, and serology. J Gastroenterol Hepatol. 2011; 26: 1053-1059. DOI: https://doi.org/10.1111/j.1440-1746.2011.06705.x
Braden B. Diagnosis of Helicobacter pylori infection. BMJ 2012; 344: e828. DOI: https://doi.org/10.1136/bmj.e828
McNulty CA, Lehours P, Mégraud F. Diagnosis of Helicobacter pylori Infection. Helicobacter. 2011; 16(Suppl 1): 10-18. DOI: https://doi.org/10.1111/j.1523-5378.2011.00875.x
Marchildon PA, Sugiyama T, Fukuda Y, Peacock JS, Asaka M, Shimoyama T, Graham DY. Evaluation of the effects of strain-specific antigen variation on the accuracy of serologic diagnosis of Helicobacter pylori infection. J Clin Microbiol. 2003; 41: 1480-1485. DOI: https://doi.org/10.1128/JCM.41.4.1480-1485.2003
Okuda M, Miyashiro E, Koike M, Tanaka T, Bouoka M, Okuda S, Yoshikawa N..Serodiagnosis of Helicobacter pylori infection is not accurate for children aged below 10. Pediatr Int. 2002; 44: 387–90. DOI: https://doi.org/10.1046/j.1442-200X.2002.01585.x
Frenck RW Jr, Fathy HM, Sherif M, Mohran Z, El Mohammedy H, Francis W, Rockabrand D, Mounir BI, Rozmajzl P, Frierson HF. Sensitivity and specificity of various tests for the diagnosis of Helicobacter pylori in Egyptian children. Pediatrics. 2006; 118: e1195–202. DOI: https://doi.org/10.1542/peds.2005-2925
Leal YA, Flores LL, GarcÃa-Cortés LB, Cedillo-Rivera R, Torres J. Antibody-based detection tests for the diagnosis of Helicobacter pylori infection in children: A meta-analysis. PLoS One. 2008; 3: e3751. DOI: https://doi.org/10.1371/journal.pone.0003751
Ueda J, Okuda M, Nishiyama T, Lin Y, Fukuda Y, Kikuchi S. Diagnostic accuracy of the E-plate serum antibody test kit in detecting Helicobacter pylori infection among Japanese children. J Epidemiol. 2014; 24(1): 47-51. DOI: https://doi.org/10.2188/jea.JE20130078
Alam El-Din HM, Hashem AG, Ragab YM, Hussein IL, Mohamed DB, Mohamed el-CB. Evaluation of noninvasive versus invasive techniques for the diagnosis of Helicobacter pylori infection. Appl Immunohistochem Mol Morphol. 2013; 21(4): 326-33. DOI: https://doi.org/10.1097/PAI.0b013e31826e4e61
Ho B, Marshall BJ. Accurate diagnosis of Helicobacter Pylori. Serologic testing. Gastroenterol Clin North Am. 2000; 29: 853–62. DOI: https://doi.org/10.1016/S0889-8553(05)70152-7
Downloads
Published
How to Cite
Issue
Section
License
http://creativecommons.org/licenses/by-nc/4.0
