Glutathione Transferase as a Potential Marker for Gut Epithelial Injury versus the Protective Role of Breast Milk sIgA in Infants with Rota Virus Gastroenteritis

Lobna S. Sherif; Randaa K. Abdel Raouf; Rokaya M. El Sayede; Amany S. El Wakkadd; Ashraf R. Shoaib; Hanan M. Ali; Amira S. El Refay

doi:10.3889/oamjms.2015.125

Authors

Lobna S. Sherif National Research Centre, Department of Child Health, Giza
Randaa K. Abdel Raouf Institute of Postgraduate Childhood Studies - Department of Medical Studies, Cairo
Rokaya M. El Sayede Kasralainy Cairo University - PÐµdiatric, Cairo
Amany S. El Wakkadd National Research Centre, Medical Physiology, Cairo
Ashraf R. Shoaib Department of Medical Studies, Institute of Postgraduate Childhood Studies, Ain Shams University, Cairo
Hanan M. Ali Department of Pediatrics, Faculty of Medicine, Cairo University, Cairo
Amira S. El Refay National Research Centre, Department of Child Health, Giza

DOI:

https://doi.org/10.3889/oamjms.2015.125

Keywords:

viral gastroenteritis, Rota virus, glutathione transferase, secretory IgA, infants

Abstract

BACKGROUND: Secretory immunoglobulin A (SIgA) plays an important protective role in the recognition and clearance of enteric pathogens.

AIM: This study was designed to assess if mucosal integrity â€œmeasured by secretory IgA (SIgA)â€ is a protective factor from more epithelial alteration â€œmeasured by glutathione transferaseâ€ in infants with Rota gastroenteritis and its relation to infantsêžŒ feeding pattern.

PATIENTS AND METHODS: This study was conducted on 79 infants aged 6 months and less from those diagnosed as having gastroenteritis and admitted to Gastroenteritis Department in Abo El Rish Pediatric Hospital, Cairo University. Plasma glutathione s-transferases and Stool SIgA were measured using ELISA technique. Rota virus detection was done by Reverse transcriptase PCR.

RESULTS: SIgA was found to be significantly positive in exclusive breast fed infants, Glutathione transferase was significantly more frequently positive in Rota positive cases than Rota negative cases by Reverse transcriptase PCR. A significant negative correlation between Glutathione transferase and Secretory IgA was found, (p < 0.05).

CONCLUSION: Breast feeding should be encouraged and highly recommended in the first two years of life as it provides Secretory IgA to breast fed infants who in turn protect them against epithelial damage caused by Rota viral gastroenteritis.

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