Comparative Hepatotoxicity of Aflatoxin B1 among Workers Exposed to Different Organic Dust with Emphasis on Polymorphism Role of Glutathione S-Transferase Gene


  • Amal Saad-Hussein Department of Environmental & Occupational Medicine, National Research Centre, Cairo
  • Eman M. Shahy Department of Environmental & Occupational Medicine, National Research Centre, Cairo
  • Weam Shaheen Department of Environmental & Occupational Medicine, National Research Centre, Cairo
  • Mona M. Taha Department of Environmental & Occupational Medicine, National Research Centre, Cairo
  • Heba Mahdy-Abdallah Department of Environmental & Occupational Medicine, National Research Centre, Cairo
  • Khadiga S. Ibrahim Department of Environmental & Occupational Medicine, National Research Centre, Cairo
  • Salwa F. Hafez Department of Environmental & Occupational Medicine, National Research Centre, Cairo
  • Nevein N. Fadl Department of Medical Physiology, National Research Centre, Cairo
  • Karima A. El-Shamy Department of Medical Physiology, National Research Centre, Cairo



Organic dust, Aflatoxin B1, Liver enzymes, GST polymorphism


AIM: The study aimed to investigate effects of organic dust exposure from different sources on aflatoxin B1-albumin adducts (AFB1/Alb), and role of glutathione S-transferase (GST) gene polymorphism in hepatotoxicity of (AFB1) among exposed workers.

MATERIAL AND METHODS: Liver enzymes, AFB1/Alb, and GST polymorphism were estimated in 132 wheat flour dust and 87 woods sawmill workers, and 156 controls.

RESULTS: Results revealed that AFB1/Alb and liver enzymes were significantly elevated in exposed workers compared to controls, and were significantly higher in sawmill workers compared to flour workers. AFB1/Alb in flour and sawmill workers with GSTT1 and GSTM1&GSTT1 null genotypes were significantly higher than controls, and in sawmill workers with GSTM1&GSTT1 null than flour workers. Liver enzymes (ALT and AST) in sawmill workers were significantly higher than flour workers and controls in all GST polymorphism; except in GSTT1 polymorphism, where these enzymes were significantly higher in the two exposed groups than controls.

CONCLUSIONS: In conclusion, organic dust exposure may cause elevation in AFB1/Alb and liver enzymes of exposed workers, and GST gene polymorphism plays an important role in susceptibility to hepatic parenchymal cell injury; except in workers with GSTT1&GSTM1 null genotype, gene susceptibility seemed to have little role and the main role was for environmental exposures.


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Douwes J, Thorne P, Pearce N, et al. Bioaerosol Health Effects and Exposure Assessment: Progress and Prospects. Ann Occup Hyg. 2003;47(3):187–200. PMid:12639832

Liebers V, Raulf-Heimsoth M, Brüning T. Health effects due to endotoxin inhalation. Arch Toxicol. 2008;82(4):203-10. PMid:18322674

Sorenson WG. Mycotoxins as potential occupational hazards. Dev Ind Microbiol. 1990; 31: 205–11.

Salvaggio JE, Neil CEO, Butcher BT. Immunologic responses to inhaled cotton dust. Environ Health Perspect. 1986;66:17–23. PMid:3519202 PMCid:PMC1474390

Saad-Hussein A, Elserougy S, Beshir S, et al. Work-Related Airborne Fungi and the Biological Levels of Mycotoxin in Textile Workers. J Appl Sci Res. 2012; 8(2): 719-26.

Lacey J, Auger P, Eduard W, et al. Tannins and mycotoxins. Am J Ind Med. 1994;25:141–4. PMid:8116643

Saad-Hussein A, Taha MM, Fadl NN, et al. Effects of Airborne Aspergillus on Serum Aflatoxin B1 and Liver Enzymes in Workers Handling Wheat Flour. Hum Exp Toxicol. 2016;35(1):3-9. PMid:25697850

Krysińska-Traczyk E, Skórska C, Cholewa G, et al. Exposure to airborne microorganisms in furniture factories. Ann Agric Environ Med. 2002; 9(1):85-90. PMid:12088403

Kosalec I, Cvek J, Tomić S. Contaminants of medicinal herbs and herbal products. Arh Hig Rada Toksikol. 2009; 60(4):485-501. PMid:20061249

Qazi J Il and Fayyaz Z. Aflatoxin contaminated foods and health risk perspective for Pakistani population. Mycopath. 2006; 4(2): 27-34.

Lizarraga-Paulin EG, Moreno-Martinez Ernesto and Susana P. Miranda-Castro Aflatoxins and Their Impact on Human and Animal Health:An Emerging Problem, Aflatoxins - Biochemistry and Molecular Biology, Dr. Ramon G. Guevara-Gonzalez (Ed.), 2011.

McLaughlin JK, Malker HS, Malker BK, et al. Registry-based analysis of occupational risks for primary liver cancer in Sweden. Cancer Res. 1987;47(1):287-91. PMid:3791212

Saad-Hussein A, Beshir S, Moubarz G, et al. Effect of Occupational Exposure to Aflatoxins on Some Liver Tumor Markers in Textile Workers. Am J Ind Med. 2013;56(7):818–24. PMid:23359393

Saad-Hussein A, Taha MM, Beshir S, et al. Carcinogenic effects of aflatoxin B1 among wheat handlers. Int Joccup Environ Health. 2014;20(3): 215-9. PMid:25000109 PMCid:PMC4090880

Hall AJ, Wild CP. Epidemiology of aflatoxin-related disease. In: Eaton DL, GroopmanmJD (eds) The toxicology of aflatoxins: human health, veterinary, and agricultural significance. Academic Press:San Diego, 1994; 233-58. PMid:24213966

Halstensen AA. Species-specific Fungal DNA in Airborne Dust as Surrogate for Occupational Mycotoxin Exposure? Int J Mol Sci. 2008, 9: 2543-58. PMid:19330091 PMCid:PMC2635655

Rumora L and Žanić-Grubišić T. A Journey Through Mitogen-Activated Protein Kinase And Ochratoxin A Interactions. Arh Hig Rada Toksikol. 2009; 60:449-56. PMid:20061246

Abd El-Moneim E, Younis F, Allam N, et al. Gene Deletion of Glutathione S-Transferase M1 and T1 and Risk Factors of Hepatocellular Carcinoma in Egyptian Patients. Egypt J Immuno. 2008;15(2): 125-34. PMid:20306695

Sreeja L, Syamala V, Hariharan S, et al. Glutathione S-transferase M1, T1 and P1 polymorphisms: susceptibility and outcome in lung cancer patients. J Exp Ther Oncol. 2008;7(1):73-85. PMid:18472644

Doumas BT, Watson WA, Biggs HG. Albumin standards and the measurement of serum albumin with bromcresol green. Clin Chim Acta. 1971;31(1):87-96.

Reitman S, Frankel S. A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. Am J Clin Pathol. 1957;28(1):56-63. PMid:13458125

Belfield A, Goldberg DM. Normal Ranges and Diagnostic Value of Serum 5′Nucleotidase and Alkaline Phosphatase Activities in Infancy. Arch Dis Child. 1971;46:842-6. PMid:5129186 PMCid:PMC1647894

Luo JC, Cheng TJ, Kuo HW, et al. Abnormal liver function associated with occupational exposure to dimethylformamide and glutathione S-transferase polymorphisms. Biomarkers. 2005;10(6):464-74. PMid:16308270

Malik A, Ali S, Shahid M, et al. Occupational exposure to Aspergillus and aflatoxins among food-grain workers in India. Int J Occup Environ Health. 2014;20(3):189-93. PMid:25000106 PMCid:PMC4090888

Yen CH, Hung JH, Ueng YF, et al. Glycine N-methyltransferase affects the metabolism of aflatoxin B1 and blocks its carcinogenic effect. Toxicol Appl Pharmacol. 2009;235(3):296-304. PMid:19146867

Liu Y, Wu F. Global Burden of Aflatoxin-Induced Hepatocellular Carcinoma: A Risk Assessment. Environ Health Perspect. 2010;118:818-824. PMid:20172840 PMCid:PMC2898859

Kew M C. Aflatoxins as a Cause of Hepatocellular Carcinoma. J Gastrointestin Liver Dis. 2013;22(3): 305-10. PMid:24078988

Mohd Redzwan S, Rosita J, Mohd Sokhini AM, et al. Detection of serum AFB1-lysine adduct in Malaysia and its association with liver and kidney functions. Int J Hyg Environ Health. 2014;217(4-5):443-51. PMid:24095591

Gouas D, Shi H, Hainau P. The aflatoxin-induced TP53 mutation at codon 249 (R249S): biomarker of exposure, early detection and target for therapy. Cancer Lett. 2009;286(1):29-37. PMid:19376640

Klarić MŠ, Varnai VM, Čalušić AL, et al. Occupational exposure to airborne fungi in two Croatian sawmills and atopy in exposed workers. Ann Agri Environ Med. 2012;19(2):213-9. PMid:22742790

Abdel Hameed AA, Khoder M I, Farag SA. Organic dust and gaseous contaminants at wood working shops. J Environ Monit. 2000;2:73-6. PMid:11256646

Chen J, Ma L, Peng NF, et al. A meta-analysis of the relationship between glutathione S-transferases gene polymorphism and hepatocellular carcinoma in Asian population. Mol Biol Rep. 2012;39:10383-93. PMid:23053942

Saad-Hussein A, El-Ghor AA, Noshy MM, et al. Hepatotoxic Effect of Aflatoxin B1 and Polymorphisms in Glutathione S-Transferase in Millers occupationally Exposed to Aspergillus. J Appl Sci Res. 2013;9(3):1724-30.

Oguz H, Hadimli HH, Kurtoglu V, et al. Evaluation of humoral immunity of broilers during chronic aflatoxin (50 and 100 ppb) and clinoptilolite exposure. Revue de Medecine Veterinaire. 2003; 154(7): 483-6.

Rom WN. Environmental and Occupational Medicine. Toxic Liver Disorders. Denise Johnson. John D. Groopman. Fourth Edition. Lippincott Williams & Wilkins, 2007.

Tao P, Zhi-Ming L, Tang-Wei L, et al. Associated factors in modulating aflatoxin B1-albumin adduct level in three Chinese populations. Dig Dis Sci. 2005;50(3):525-32. PMid:15810636

Obuseh FA, Jolly PE, Jiang Y, Shuaib FM, Waterbor J, Ellis WO, Piyathilake CJ, Desmond RA, Afriyie-Gyawu E, Phillips TD. Aflatoxin B1 albumin adducts in plasma and aflatoxin M1 in urine are associated with plasma concentrations of vitamins A and E. Int J Vitam Nutr Res. 2010;80(6):355-68. PMid:21792816 PMCid:PMC3222292

Higashi N, Sato M, Kojima N, et al. Vitamin A storage in hepatic stellate cells in the regenerating rat liver: with special reference to zonal heterogeneity. Anat Rec A Discov Mol Cell Evol Biol. 2005;286: 899-907. PMid:16086432

Peng T, Li LQ, Peng MH, et al. Evaluation of oxidative stress in a group of adolescents exposed to a high level of aflatoxin B1--a multi-center and multi-biomarker study. Carcinogenesis. 2007;28(11):2347-54. PMid:17724371

Wild CP, Fortuin M, Donato F, et al. Aflatoxin, liver enzymes, and hepatitis B virus infection in Gambian children. Cancer Epidemiol Biomarkers Prev. 1993;2: 555-61. PMid:8268773



How to Cite

Saad-Hussein A, Shahy EM, Shaheen W, Taha MM, Mahdy-Abdallah H, Ibrahim KS, Hafez SF, Fadl NN, El-Shamy KA. Comparative Hepatotoxicity of Aflatoxin B1 among Workers Exposed to Different Organic Dust with Emphasis on Polymorphism Role of Glutathione S-Transferase Gene. Open Access Maced J Med Sci [Internet]. 2016 Apr. 20 [cited 2022 Aug. 14];4(2):312-8. Available from:



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