No Association between 25 (OH) Vitamin D Level And Hypothyroidism among Females


  • Imad R. Musa Armed Forces Hospital at King Abdu Aziz Air Base, Dhahran
  • Gasim I. Gasim Faculty of Medicine, Al Neelain University, Khartoum
  • Sajjad Khan Central Hospital, Arar
  • Ibrahim A. Ibrahim Medical Laboratory Science, United Medical Laboratory
  • Hamdi Abo-alazm Medical Laboratory Science, United Medical Laboratory
  • Ishag Adam Faculty of Medicine, Khartoum University, Khartoum



Keywords, hypothyroidism, vitamin D levels, females


AIM: The aim was to investigate serum vitamin D (25-OH) level among females with hypothyroidism.

MATERIALS AND METHODS: A case-control study (58 in each arm) was conducted in Arar Central Hospital, Kingdom Saudi Arabia. The cases were females with hypothyroidism, and healthy females were controls. TSH, thyroid hormones: Free T3 (FT3) and Free T4 (FT4) and haemoglobin levels were measured in all participants. Serum vitamin D (25-OH) level was measured using the spectrophotometry.

RESULTS: While there was no significant difference in the age and haemoglobin level, body mass index (BMI) was significantly higher in the cases. Compared with the controls, cases had significantly higher TSH, had significantly lower T4, and there was no significant difference in FT3 and 25 (OH) vitamin D, [16.1 (8.8-26.7) vs. 14.0 (9.5-20.3 ng/ml; P = 0.577]. Linear regression showed no association between, age, BMI, haemoglobin, TSH, FT3, FT4 and the log of 25 (OH) vitamin D levels.

CONCLUSION: There was no significant difference in vitamin D level among females with hypothyroidism and healthy controls.


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Holick MF. Vitamin D Deficiency. New England Journal of Medicine. 2007; 357: 266–281. PMid:17634462

Holick MF, Chen TC. Vitamin D deficiency: a worldwide problem with health consequences. The American journal of clinical nutrition. 2008; 87:1080S–6S. PMid:18400738

Lemire JM, Adams JS, Sakai R, Jordan SC. 1 alpha,25-dihydroxyvitamin D3 suppresses proliferation and immunoglobulin production by normal human peripheral blood mononuclear cells. Journal of Clinical Investigation. 1984;74(2):657-661. PMid:6611355 PMCid:PMC370520

Rigby WF, Stacy T, Fanger MW. Inhibition of T lymphocyte mitogenesis by 1, 25-dihydroxyvitamin D3 (calcitriol). The Journal of clinical investigation. 1984; 74:1451–5. PMid:6332829 PMCid:PMC425314

Kostoglou-Athanassiou I, Ntalles K. Hypothyroidism - new aspects of an old disease. Hippokratia. 2010;14(2):82-87. PMid:20596261 PMCid:PMC2895281

Mosekilde L, Lund B, So̸rensen OH, Christensen MS, Melsen F. Serum-25 hydroxycholecalciferol in hyperthyroidism. The Lancet. 1977; 309:806–807.

Velentzas C, Oreopoulos D.G, From G, Porret B, Rapoport A. Vitamin-d levels in thyrotoxicosis. The Lancet. 1977; 309:370–371.

Alhuzaim ON, Aljohani N. Effect of Vitamin D3 on Untreated Graves' Disease with Vitamin D Deficiency. Clinical Medicine Insights Case Reports. 2014; 7:83-85. PMid:25187748 PMCid:PMC4133032

Lee JH, O'Keefe JH, Bell D, Hensrud DD, Holick MF. Vitamin D deficiency an important, common, and easily treatable cardiovascular risk factor? Journal of the American College of Cardiology. 2008;52:1949–56. PMid:19055985

Melamed ML, Michos ED, Post W, Astor B. 25-hydroxyvitamin D levels and the risk of mortality in the general population. Archives of internal medicine, 2008; 168:1629–37. PMid:18695076 PMCid:PMC2677029

Almerighi C, Sinistro A, Cavazza A, Ciaprini C, Rocchi G, Bergamini A. 1Alpha,25-dihydroxyvitamin D3 inhibits CD40L-induced pro-inflammatory and immunomodulatory activity in human monocytes. Cytokine. 2009;45(3):190–7. PMid:19186073

Lips P. Vitamin D physiology. Progress in Biophysics and Molecular Biology. 2006; 92:4–8. PMid:16563471

Yetley EA, Brulé D, Cheney MC, Davis CD, Esslinger KA, Fischer PW et al. Dietary reference intakes for vitamin D: justification for a review of the 1997 values. The American journal of clinical nutrition. 2009; 89:719–27. PMid:19176741 PMCid:PMC2667666

Jones G. Pharmacokinetics of vitamin D toxicity. The American journal of clinical nutrition. 2008; 88:582S–586S. PMid:18689406

Alqahatani M, Tamimi W, Aldaker M, Alenzi F, Tamim H, Alsadhan A. Young adult reference ranges for thyroid function tests on the Centaur immunoassay analyser. British journal of biomedical science. 2006;63:163–5. PMid:17201204

Khan QJ, Fabian CJ. How I treat vitamin d deficiency. Journal of oncology practice. 2010; 6:97–101. PMid:20592785 PMCid:PMC2835491

Goswami R, Marwaha RK, Gupta N, Tandon N, Sreenivas V, Tomar N, et al. Prevalence of vitamin D deficiency and its relationship with thyroid autoimmunity in Asian Indians: a community-based survey. British Journal of Nutrition. 2009;102: 382. PMid:19203420

Effraimidis G, Badenhoop K, Tijssen JG, Wiersinga WM. Vitamin D deficiency is not associated with early stages of thyroid autoimmunity. European journal of endocrinology / European Federation of Endocrine Societies. 2012; 167:43–8. PMid:22518050

Sezgin G, Ozer ME, Bayramicli OU, Ozel AM, Aksungar F, Nalbant S. Relationship of vitamin d deficiency and autoimmune thyroid diseases. European Journal of Internal Medicine. 2011; 22:S87.

Hussain AN, Alkhenizan AH, El Shaker M, Raef H, Gabr A. Increasing trends and significance of hypovitaminosis D: a population-based study in the Kingdom of Saudi Arabia. Arch Osteoporos. 2014;9(1):190. PMid:25213798

Alkhenizan A, Mahmoud A, Hussain A, Gabr A, Alsoghayer S, Eldali A. The Relationship between 25 (OH) D Levels (Vitamin D) and Bone Mineral Density (BMD) in a Saudi Population in a Community-Based Setting. Slominski AT, editor. PLoS One. 2017;12(1):e0169122.

Sadat-Ali M, AlElq A, Al-Turki H, Al-Mulhim F, Al-Ali A. Vitamin D levels in healthy men in eastern Saudi Arabia. Annals of Saudi Medicine. 2009;29:378-382. PMid:19700896 PMCid:PMC3290044

Fawzy E, Al-Sayed Mohamed S, El-Rabat AM. Hypovitaminosis D In Autoimmune Hypothyroidism. Journal of American Science J Am Sci. 2013; 99:60–65.

Mackawy AMH, Al-ayed Bushra Mohammed, Al-rashidi Bashayer Mater. Vitamin D Deficiency and Its Association with Thyroid Disease. International Journal of Health Sciences. 2013;7(3):267-275. PMid:24533019 PMCid:PMC3921055

Boyuk B, Altay M, Atalay H, Guzel S, Celebi A, Ekizoglu I. No relationship between 25 OH vitamin D and autoimmunity in women patients with hypothyroidism. Acta Medica Mediterr. 2016;32(3):779–84.

Kivity S, Agmon-Levin N, Zisappl M, Shapira Y, Szekanecz Z. Thyroid disease and vitamin D deficiency. Annals of the Rheumatic Diseases. 2010;69:A62–A62.

Baeke F, Takiishi T, Korf H, Gysemans C, Mathieu C. Vitamin D: modulator of the immune system. Current opinion in pharmacology. 2010;10:482–96. PMid:20427238

Tamer G, Arik S, Tamer I, Coksert D. Relative vitamin D insufficiency in Hashimoto's thyroiditis. Thyroid : official journal of the American Thyroid Association. 2011; 21:891–6. PMid:21751884

Shin DY, Kim KJ, Kim D, Hwang S, Lee EJ. Low serum vitamin D is associated with anti-thyroid peroxidase antibody in autoimmune thyroiditis. Yonsei medical journal, 2014; 55:476–81. PMid:24532520 PMCid:PMC3936621

Choi YM, Kim WG, Kim TY, Bae SJ, Kim HK, Jang EK. et al. Low levels of serum vitamin D3 are associated with autoimmune thyroid disease in pre-menopausal women. Thyroid : official journal of the American Thyroid Association. 2014; 24:655–61. PMid:24320141 PMCid:PMC3993051

Wang J, Lv S, Chen G, Gao C, He J, Zhong H, Xu Y. et al. Meta-Analysis of the Association between Vitamin D and Autoimmune Thyroid Disease. Nutrients. 2015; 7:2485-2498. PMid:25854833 PMCid:PMC4425156

Aljohani NJ, Al-Daghri NM, Al-Attas OS, Alokail MS, Alkhrafy KM, Al-Othman A, Yakout S. et al. Differences and associations of metabolic and vitamin D status among patients with and without sub-clinical hypothyroid dysfunction. BMC endocrine disorders. 2013;13:31. PMid:23962199 PMCid:PMC3751774

Al-Daghri NM, Al-Attas OS, Al-Okail MS, Alkharfy KM, Al-Yousef MA, Nadhrah HM. et al. Severe hypovitaminosis D is widespread and more common in non-diabetics than diabetics in Saudi adults. Saudi medical journal. 2010;31:775–80. PMid:20635011

Tamer G, Mesci B, Tamer I, Kilic D, Arik S. Is vitamin D deficiency an independent risk factor for obesity and abdominal obesity in women? Endokrynologia Polska. 2012;63:196–201. PMid:22744625

Saler T, Keşkek SO, Ahbab S, Cakir S, Ortoğlu G, Bankir M. Frequency of Hashimoto's thyroiditis in women with vitamin D deficiency: A cross sectional study. American Journal of Internal Medicine. 2014; 2:44–48.

Zhang Q, Wang Z, Sun M, Mengdie Cao, Zhenxin Zhu, Qi Fu ,et al. Association of High Vitamin D Status with Low Circulating Thyroid-Stimulating Hormone Independent of Thyroid Hormone Levels in Middle-Aged and Elderly Males. International Journal of Endocrinology. 2014;2014:631819. PMid:24693286 PMCid:PMC3947886

Kim, D. Low vitamin D status is associated with hypothyroid Hashimoto's thyroiditis. Hormones (Athens, Greece). 2016;15(3):385-393.

Barchetta I, Baroni MG, Leonetti F, De Bernardinis M, Bertoccini L, Fontana M. et al. TSH levels are associated with vitamin D status and seasonality in an adult population of euthyroid adults. Clinical and experimental medicine. 2015;15:389–96. PMid:24925636

Vondra K, Stárka L, Hampl R. Vitamin D and Thyroid Diseases. Physiol. Res. 2015; 64:95–100.

Chaudhary S, Dutta D, Kumar M, Saha S, Mondal SA, Kumar A et al. Vitamin D supplementation reduces thyroid peroxidase antibody levels in patients with autoimmune thyroid disease: An open-labeled randomized controlled trial. Indian journal of endocrinology and metabolism. 2016; 20:391–8. PMid:27186560 PMCid:PMC4855971

Mansournia N, Mansournia MA, Saeedi S, Dehghan J. The association between serum 25OHD levels and hypothyroid Hashimoto's thyroiditis. Journal of endocrinological investigation. 2014; 37:473–6. PMid:24639121

Bársony J, Lakatos P, Földes J, Fehér T. Effect of vitamin D3 loading and thyroid hormone replacement therapy on the decreased serum 25-hydroxyvitamin D level in patients with hypothyroidism. Acta endocrinologica. 1986; 113:329–34.

Sworczak K, Wiśniewski P. The role of vitamins in the prevention and treatment of thyroid disorders. Endokrynologia Polska. 2011; 62:340–4. PMid:21879475

Clinckspoor I, Verlinden L, Mathieu C, Bouillon R, Verstuyf A, Decallonne B. Vitamin D in thyroid tumorigenesis and development. Progress in Histochemistry and Cytochemistry. 2013; 48:65–98. PMid:23890557

Penna-Martinez M, Ramos-Lopez E, Stern J, Kahles H, Hinsch N, Hansmann ML, Selkinski I, et al. Impaired Vitamin D Activation and Association with CYP24A1 Haplotypes in Differentiated Thyroid Carcinoma. Thyroid. 2012; 22:709–716. PMid:22690899 PMCid:PMC3387756

Haney EM, Stadler D, Bliziotes MM. Vitamin D insufficiency in internal medicine residents. Calcified tissue international. 2005;76: 11–6.




How to Cite

Musa IR, Gasim GI, Khan S, Ibrahim IA, Abo-alazm H, Adam I. No Association between 25 (OH) Vitamin D Level And Hypothyroidism among Females. Open Access Maced J Med Sci [Internet]. 2017 Mar. 19 [cited 2021 Sep. 20];5(2):126-30. Available from:



A - Basic Science

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