Eosinophil Cationic Protein Concentrations among Crop and Dairy Farmers with Asthma
DOI:
https://doi.org/10.3889/oamjms.2018.113Keywords:
Airway inflammation, Asthma, Farmers, Respiratory symptomsAbstract
OBJECTIVE: To assess the mean serum eosinophil cationic protein (s-ECP) concentrations among crop and dairy farmers and office controls, and further examine its relation to exposure duration, smoking habit, as well as presence or absence of asthma.
METHODS: A cross-sectional survey was performed including examined group (EG), composed by agricultural workers (87 crop - EG1 and 83 dairy farmers - EG2), and control group (CG) composed by 80 office workers within the same enterprise. Â We have used a questionnaire to record the chronic respiratory symptoms, detailed work history, specific farming activities and tasks performed and smoking history. Evaluation of examined subjects also included lung function tests, diagnosis of asthma, and measurement of s-ECP as a marker of inflammation.
RESULTS: The main finding of the present study is that s-ECP concentrations were raised in subjects with asthma independent of the smoking habit. The mean s-ECP concentrations were higher in subjects of EG1 and EG2 compared with those in CG, but without reaching statistical significance. Mean s-ECP concentrations were significantly higher among subjects in EG1 exposed more than 20 years, while mean s-ECP concentrations were non-significantly higher in subjects of EG2 exposed more than 20 years, compared to those exposed less than 20 years. Mean s-ECP concentrations were higher among smokers within all three groups, but without reaching statistical significance between smokers and non-smokers. Mean s-ECP concentrations were significantly higher in subjects with asthma within EG1 (P = 0.049) and EG2 (P = 0.040), but also within those in CG (P = 0.046).
CONCLUSION: Data obtained suggest that airway inflammation is present in farmers with asthma, and s-ECP is an important biomarker in means of reflecting disease severity and prognosis among exposed workers.Downloads
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Lieberman P. Objective measures of asthma control: sputum eosinophils, nitric oxide, and other inflammatory mediators. Allergy and Asthma Proceedings. 2007; 28:510-513. https://doi.org/10.2500/aap2007.28.3039 PMid:18034966
Koh GC et al. Eosinophil cationic protein: is it useful in asthma? A systematic review. Respiratory medicine. 2007; 101:696-705. https://doi.org/10.1016/j.rmed.2006.08.012 PMid:17034998
Romagnoli M, Vachier I, Taroda P, et al. Eosinophilic inflammation in sputum of poorly controlled asthma. Eur Respir J. 2002; 20:1370-1377. https://doi.org/10.1183/09031936.02.00029202 PMid:12503691
Rylander R. Organic dust and lung disease: the role of inflammation. Ann AgricEnviron Med. 1994; 1:7-10.
Melbostad E, Eduard W, Magnus P. Determinants of asthma in a farming population. Scand J Work Environ Health. 1998; 24:262-9. https://doi.org/10.5271/sjweh.319 PMid:9754857
Mapp CE, Plebani M, Faggian D, Maestrelli P, Saetta M, Calcagni P, Borghesan F, Fabbri LM. Eosinophil cationic protein (ECP), histamine and tryptase in peripheral blood before and during inhalation challenge with toluene diisocyanate (TDI) in sensitized subjects. Clin Exp Allergy. 1994; 24:730-6. https://doi.org/10.1111/j.1365-2222.1994.tb00983.x PMid:7982122
Venge P. Serum measurements of eosinophil cationic protein (ECP) in bronchial asthma. Clin Exp Allergy. 1993; 23 Suppl 2:3-7. https://doi.org/10.1111/j.1365-2222.1993.tb00372.x PMid:8364772
Melbostad E, Venge P, Danielsen A, Kjuus H. Serum eosinophil cationic protein in asthma of farmers. Scand J Clin Lab Invest. 2000; 60:111-118. https://doi.org/10.1080/00365510050184921 PMid:10817397
Park C, Lundahl J, Hallden G, Bylin G. Total ECP levels in induced sputum as a market of eosinophilic inflammation in patients with allergic asthma. Ann Allergy Asthma Immunol. 2005; 95:86-92. https://doi.org/10.1016/S1081-1206(10)61193-3
Virchow JC, Holscher U, Virchow C. Sputum ECP levels correlates with parameters of airflow obstruction. Am Rev Respir Dis. 1992; 146:604-606. https://doi.org/10.1164/ajrccm/146.3.604 PMid:1519835
Fujimoto K, Kubo K, Matsuzawa Y, Sekiguchi M. Eosinophil cationic protein levels in induced sputum correlate with the severity of bronchial asthma. Chest. 1997; 112(5):1241-7. https://doi.org/10.1378/chest.112.5.1241 PMid:9367463
Onadeko BO, Khadadah ME, Mustafa HT, Ezeamuzie CI, Marouf R, Khamis A, et al. Serum eosinophil cationic protein as a predictor of disease activity in acute and chronic asthma. East Afr Med J. 1999; 76:524-9. PMid:10685325
Sorkness C, McGill K, Busse WW. Evaluation of serum eosinophil cationic protein as a predictive marker for asthma exacerbation in patients with persistent disease. Clin Exp Allergy. 2002; 32:1355-1359. https://doi.org/10.1046/j.1365-2222.2002.01471.x PMid:12220475
Di Gioacchino M, Cavallucci E, Di Stefano F, Verna N, Ramondo S, Ciuffreda S, Riccioni G, Boscolo P. Infuence of total IgE and seasonal increase of eosinophil cationic protein on bronchial hyperreactivity in asthmatic grass-sensitized farmers. Allergy. 2000; 55:1030-1034. https://doi.org/10.1034/j.1398-9995.2000.00676.x PMid:11097312
Samarai A, Al Obaidi A, Al- Janabi J, Al Samarai A. Role of Eosinophil Cationic Protein in Asthma and Confounding Factors. The Internet Journal of Asthma, Allergy and Immunology. 2008; 7(2):1-9.
Stoleski S, Minov J, Karadzinska-Bislimovska J, Mijakoski D, Tutkun L. C-reactive protein concentrations among crop and dairy farmers with stable chronic obstructive pulmonary disease. Open Access Maced J Med Sci. 2017; 5(6):724-729. https://doi.org/10.3889/oamjms.2017.140
Minette A. Questionnaire of the European Community for Coal and Steel (ECSC) on respiratory symptoms. 1987 - Updating of the 1962 and 1967 questionnaires for studying chronic bronchitis and emphysema. Eur Respir J. 1989; 2:165- 177. PMid:2703044
European Community Respiratory Health Survey. Variations in the prevalence of respiratory symptoms, self-reported asthma attacks, and use of asthma edication in the European Respiratory Health Survey (ECRHS). Eur Respir J. 1996; 9:687-695. https://doi.org/10.1183/09031936.96.09040687
World Health Organization. Guidelines for controlling and monitoring the tobacco epidemic. Geneva: WHO, 1998.
Smoking Pack-Years. Available from: http://smokingpackyears.cm/calculate. Accessed 15 November 2017.
US Department of Health and Human Services. The health consequences of smoking: chronic obstructive pulmonary disease. A report of the Surgeon General. US Department of Health and Human Services, Public Health Service, Office of the Assistant for Health, Office of Smoking and Health. DHHS Publication No. 84-50 205,1984.
Quajner PhH, ed. Standardization of Lung Function Tests - 1993 Update. Report Working Party for the European Community for Steel and Coal. Official Statement of the European Respiratory Society. Eur Rrespir J. 1993; 16(l):1-100.
Global Initiative for Asthma (GINA). GINA Report 2014, Global Strategy for Asthma Management and Prevention. http://www.ginasthma.org. Updated 2017.
D'Amato G et al. Measurement of serum levels of eosinophil cationic protein to monitor patients with seasonal respiratory allergy induced by Parietaria pollen (treated and untreated with specific immunotherapy). Allergy. 1996; 51:245-250. https://doi.org/10.1111/j.1398-9995.1996.tb04600.x PMid:8792921
Heldal К.К, Halstensen A.S, Thorn J, Djupesland P, Wouters I, Eduard W, Halstensen T.S. Upper airway inflammation in waste handlers exposed to bioaerosols. Occup Environ Med. 2003; 60:444-450. https://doi.org/10.1136/oem.60.6.444 PMCid:PMC1740561
Sorkness C, McGill K, Busse WW. Evaluation of serum eosinophil cationic protein as a predictive marker for asthma exacerbation in patients with persistent disease. Clin Exp Allergy. 2002; 32:1355-1359. https://doi.org/10.1046/j.1365-2222.2002.01471.x PMid:12220475
Parra A, Sanz ML, Vila L, Prieto I, Dieguez I, Oehling AK. Eosinophil soluble protein levels, eosinophil peroxidase and eosinophil cationic protein in asthmatic patients. J Investig Allergol Clin Immunol. 1999; 9:27-34. PMid:10212854
Dal Negro R, Micheletto C, Tognella S, Mauroner L, Burti E, Turco P et al. Effect of inhaled beclomethasone dipropionate and budesonide dry powder on pulmonary function and serum eosinophil cationic protein in adult asthmatics. J Investig Allergol Clin Immunol. 1999; 9:241-247. PMid:10513351
Nakazawa T, Kagami M, Matumura R, Kawashima T, Matuzuwa Y, Takada M. Sensitivity of sputum eosinophil cationic protein level for monitoring asthmatic patients with normal peak expiratory flow. Arerugy.1999; 48:1153-1160.
Di Lorenzo G, Drago A, Pellitteri ME, Candore G, Colombo A, Potestio M, Di Salvo A, Mansueto S, Caruso C. Serum levels of soluble CD23 in patients with asthma or rhinitis monosensitive to Parietaria. Its relation to total serum IgE levels and eosinophil cationic protein during and out of the pollen season. Allergy Asthma Proc. 1999; 20:119-125. https://doi.org/10.2500/108854199778612590 PMid:10209690
Zubovic I, Rozmanic V, Ahel V, Banac S. Manifold significance of serum eosinophil cationic protein in asthmatic children. Acta Med Croatica. 2002; 56:53-56. PMid:12596625
Niimi A, Amitani R, Suzuki K, Tanaka E, Murayama T, Kuze F. Serum eosinophil cationic protein as a marker of eosinophilic inflammation in asthma. Clin Exp Allergy. 1998; 28:233-240. https://doi.org/10.1046/j.1365-2222.1998.00217.x PMid:9515598
Nieto A. What role does ECP have in the evaluation of asthma severity? Allergol Immunopathol. 2000; 28:119-124.
Koller DY, Halmerbauer G, Frischer T, Roithner R. Assessment of eosinophil granules protein in various body fluids: is there a relation to clinical variables in childhood asthma? Clin Exp Allergy. 1999; 29:786-793. https://doi.org/10.1046/j.1365-2222.1999.00546.x PMid:10336595
Perfetti L, Gald E, Brame B, Speciale L, Moscato G. Serum ECP in subjects with a history of asthma symptoms with or without rhinitis. Allergy. 1999; 54:962-967. https://doi.org/10.1034/j.1398-9995.1999.00023.x PMid:10505459
Piacentini GI, Bodini A, Costella S, et al. Exhaled nitric oxide , serum ECP and airway responsiveness in mild asthmatic children. Eur Respir J. 2000; 15:839-843. https://doi.org/10.1034/j.1399-3003.2000.15e05.x PMid:10853846
Matsumato H, Niimi A, Minakuchi M, Izumi T. Serum ECP levels measured during exacerbation of asthma: characteristics of patients with lower titer. Clin Exp Allergy. 2001; 32:1355-1359.
Vignola AM, Chanez P, Campbell AM, et al. Airway inflammation in mild intermittent and persistent asthma. Am J Respir Crit Care Med. 1998; 157:403-409. https://doi.org/10.1164/ajrccm.157.2.96-08040 PMid:9476850
Löwhagen O, Wever M.J, Lusuardi M, Moscato G, De Backer W.A, Gandola L, Donner C.F, Ahlstedt S, Larsson L, Holgate S.T. The inflammatory marker serum eosinophil cationic protein (s-ECP) compared with PEF as a tool to decide inhaled corticosteroid dose in asthmatic patients. Respiratory medicine. 2002; 96:95-101. https://doi.org/10.1053/rmed.2001.1218 PMid:11862965
Zedan M, Settin A, El-Chennawi F, El-Desouky T, Nasef N, Fouda A. Eosinophilic cationic protein: is it useful in assessing control of childhood asthma? East Mediterr Health J. 2010; 16(10):1045-9. PMid:21222420
Kaiser HB. Compliance and noncompliance in asthma. Allergy and Asthma Proceedings. 2007; 28:514-516. https://doi.org/10.2500/aap2007.28.3040 PMid:18034967
Lee MH et al. Serum eosinophilic cationic protein levels and bronchodilator response at acute asthma exacerbation. Annals of Allergy, Asthma and Immunology. 1997; 79:363-369. https://doi.org/10.1016/S1081-1206(10)63029-3
Melbostad E, Venge P, Danielsen A, Kjuus H. Serum eosinophil cationic protein in asthma of farmers. Scand J Clin Lab Invest. 2000; 60:111-118. https://doi.org/10.1080/00365510050184921 PMid:10817397
Dimitrievska D, Zdraveska M, Todevski D, Jovanovska-Janeva E, Debreslioska A. Biomarkers of inflammation in peripheral blood during treatment with inhaled corticosteroids in patients with asthma. SEE J Immunol. 2016; 2016:20010. https://doi.org/10.3889/seejim.2016.20010
Goseva Z, Jovanovska Janeva E, Gjorcev A, Arsovski Z, Pejkovska S. Role and significance of markers of inflammation in the asthmatic disease. Open Access Maced J Med Sci. 2015; 3(4):630-634. https://doi.org/10.3889/oamjms.2015.109 PMid:27275299 PMCid:PMC4877899
Loutsios C, Farahi N, Porter L, Lok LS, Peters AM, Condliffe AM, Chilvers ER. Biomarkers of eosinophilic inflammation in asthma. Expert Rev Respir Med. 2014; 8(2):143-50. https://doi.org/10.1586/17476348.2014.880052 PMid:24460178
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