The Immunomodulatory Activities of Picria Fel-Terrae Lour Herbs towards RAW 264.7 Cells

Authors

  • Novycha AuliaFendri Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia
  • - Rosidah Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia
  • - Yuandani Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia
  • Sri Suryani Department of Biochemistry, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia
  • Denny Satria Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia

DOI:

https://doi.org/10.3889/oamjms.2019.017

Keywords:

Picria fel-terrae Lour, inflammatory biomarkers, Cell viability, MTT assay, Reverse Transcription-Polymerase Chain Reaction (RT-PCR)

Abstract

AIM: To investigate immunomodulatory activities of Picria fel-terrae Lour herbs extract against inflammatory biomarkers by conducting cell culture experiments.

MATERIAL AND METHODS: The herbs of Picria fel-terrae Lour were dried and extracted with n-hexane, ethyl acetate, 96% ethanol, followed by evaporation and freeze-drying. Phytochemicals screening were analysed with thin layer chromatography method. Cell viability was assessed with MTT assay. The genes of Tumor Necrosis Factor (TNF)-α, Interleukin (IL)-6, interleukin (IL)-1β and inducible Nitric Oxide Synthase (iNOS), Cyclooxygenase (COX-2) in lipopolysaccharide (LPS)-induced macrophages were analysed by Reverse Transcription-Polymerase Chain Reaction (RT-PCR) method.

RESULTS: Phytochemicals screening showed the presence of steroids in n-hexane extract (ENPFH) and flavonoids, glycosides, saponins, tannins in ethyl acetate (EEAPFH) and ethanol (EEPFH) extracts. The Viability of RAW 264.7 cell toward ENPFH, EEAPFH, and EEPFH (1-200 μgmL-1) showed no toxicity effects. At the gene level, ENPFH; EEAPFH; EEPFH decreased the gene expression of TNF-α, IL-6, IL-1β, iNOS, and COX-2 which induced with LPS (1 μgmL-1).

CONCLUSIONS: Our results suggest that extracts of Picria fel-terrae Lour Herbs possesses immunomodulatory activities by inhibiting selected inflammatory biomarkers at the gene levels in LPS-induced macrophages.

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Published

2019-01-05

How to Cite

1.
AuliaFendri N, Rosidah -, Yuandani -, Suryani S, Satria D. The Immunomodulatory Activities of Picria Fel-Terrae Lour Herbs towards RAW 264.7 Cells. Open Access Maced J Med Sci [Internet]. 2019 Jan. 5 [cited 2024 Jul. 23];7(1):24-8. Available from: https://oamjms.eu/index.php/mjms/article/view/oamjms.2019.017

Issue

Section

A - Basic Science

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