Cell Cycle Inhibition of Ethylacetate Fraction of Zanthoxylum Acanthopodium DC. Fruit against T47D Cells


  • Denny Satria Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia
  • Jansen Silalahi Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia
  • Ginda Haro Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia
  • Syafruddin Ilyas Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sumatera Utara, Medan, Indonesia
  • Poppy Anjelisa Zaitun Hasibuan Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia




cytotoxic, ethylacetate, cell cycle inhibition, Zanthoxylum acanthopodium DC


BACKGROUND: The use of medicinal plants is increasing in several decades for relief many diseases. Indonesia consists of thousands of islands with various plants and the manners of the community using plants as a treatment for every disease traditionally.

AIM: Cytotoxic activity of ethyl acetate fraction (EAF) of Zanthoxylum acanthopodium fruit was tested towards T47D breast cancer cells.

METHODS: The in vitro cytotoxic activity was performed by MTT assay, and the result was expressed as the IC50 (Inhibitory Concentration), and cell cycle inhibition was investigated by flow cytometry to assess the inhibition in every phase of cell cycle, and the role of expression cyclin D1 and p53 in cell cycle inhibition were performed by immunocytochemistry.

RESULTS: EAF was showed to have high activity with a value of IC50 48.94 ± 0.32 µg/mL. EAF of 25 µg/mL caused cell accumulation at G0/G1 (60.48%) and in a control cell (51.69%) and decreased expression of cyclin D1 and increased expression of p53.

CONCLUSION: The results obtained in this study provided scientific support for further investigation on compounds in Z. acanthopodium fruit which in the future could be used for medication.


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How to Cite

Satria D, Silalahi J, Haro G, Ilyas S, Hasibuan PAZ. Cell Cycle Inhibition of Ethylacetate Fraction of Zanthoxylum Acanthopodium DC. Fruit against T47D Cells. Open Access Maced J Med Sci [Internet]. 2019 Mar. 15 [cited 2024 May 26];7(5):726-9. Available from: https://oamjms.eu/index.php/mjms/article/view/oamjms.2019.178



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