Low Degree Hyaluronic Acid Crosslinking Inducing the Release of TGF-Β1 in Conditioned Medium of Wharton’s Jelly-Derived Stem Cells


  • Nora Ariyati Doctoral Program, Universitas Brawijaya, Malang, Indonesia
  • Kusworini Kusworini Department of Clinical Pathology, Universitas Brawijaya, Malang, Indonesia
  • Nurdiana Nurdiana Department of Pharmacology, Universitas Brawijaya, Malang, Indonesia
  • Yohanes Widodo Wirohadidjojo Department of Dermatology and Venereology, Universitas Gadjah Mada, Yogyakarta, Indonesia




Conditioned medium, Wharton’s jelly stem cells, Hyaluronic acid crosslinking, Transforming growth factor-β1


BACKGROUND: Presently, the application of stem cells and their paracrine effect for anti-ageing therapy has commenced. Wharton's jelly-derived stem cell conditioned medium (WJSCs-CM) is renowned for increasing proliferation, migrate ageing skin fibroblasts and increase consumption of extracellular transforming growth factor-β (TGF-β). With more than 85% of frequently used dermal filler procedures are hyaluronic acid fillers (HA), a mixture of both with optimal HA crosslinking degree has not yet been identified.

AIM: This study aimed to determine the discrepancies in the results of various HA crosslinking degree in WJSCs-CM concerning various levels of growth factors (GF).

METHODS: Conditioned medium was obtained from mesenchymal stem cells Wharton’s jelly of the newborn umbilical cord with caesarean section procedure, fabricated with hypoxia method (HCM). HA was obtained from preparations on the market with crosslinking degrees of 3%, 4%, and 10%. GF levels were measured using sandwich ELISA method based on the protocol provided by anti-TGF-β1, platelet-derived growth factor (PDGF), and basic fibroblast growth factor (bFGF) antibody producers (Cloud-Clone Corp®, Texas, USA).

RESULTS: Low degree HA crosslinking (3% and 4%) elevated TGF-β1 release in WJSCs-CM. HA crosslinking did not provoke increased levels of PDGF and bFGF in WJSCs-CM, both at low and higher degrees.

CONCLUSION: Low degree HA crosslinking induced the increase of TGF-β1 release in WJSCs-CM.


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How to Cite

Ariyati N, Kusworini K, Nurdiana N, Wirohadidjojo YW. Low Degree Hyaluronic Acid Crosslinking Inducing the Release of TGF-Β1 in Conditioned Medium of Wharton’s Jelly-Derived Stem Cells. Open Access Maced J Med Sci [Internet]. 2019 May 14 [cited 2021 May 9];7(10):1572-5. Available from: https://oamjms.eu/index.php/mjms/article/view/oamjms.2019.307



A - Basic Science