Low Degree Hyaluronic Acid Crosslinking Inducing the Release of TGF-Î’1 in Conditioned Medium of Whartonâ€™s Jelly-Derived Stem Cells
Keywords:Conditioned medium, Whartonâ€™s jelly stem cells, Hyaluronic acid crosslinking, Transforming growth factor-Î²1
BACKGROUND: Presently, the application of stem cells and their paracrine effect for anti-ageing therapy has commenced. Wharton's jelly-derived stem cell conditioned medium (WJSCs-CM) is renowned for increasing proliferation, migrate ageing skin fibroblasts and increase consumption of extracellular transforming growth factor-Î² (TGF-Î²). With more than 85% of frequently used dermal filler procedures are hyaluronic acid fillers (HA), a mixture of both with optimal HA crosslinking degree has not yet been identified.
AIM: This study aimed to determine the discrepancies in the results of various HA crosslinking degree in WJSCs-CM concerning various levels of growth factors (GF).
METHODS: Conditioned medium was obtained from mesenchymal stem cells Whartonâ€™s jelly of the newborn umbilical cord with caesarean section procedure, fabricated with hypoxia method (HCM). HA was obtained from preparations on the market with crosslinking degrees of 3%, 4%, and 10%. GF levels were measured using sandwich ELISA method based on the protocol provided by anti-TGF-Î²1, platelet-derived growth factor (PDGF), and basic fibroblast growth factor (bFGF) antibody producers (Cloud-Clone CorpÂ®, Texas, USA).
RESULTS: Low degree HA crosslinking (3% and 4%) elevated TGF-Î²1 release in WJSCs-CM. HA crosslinking did not provoke increased levels of PDGF and bFGF in WJSCs-CM, both at low and higher degrees.
CONCLUSION: Low degree HA crosslinking induced the increase of TGF-Î²1 release in WJSCs-CM.
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