Effect of Thymoquinone and Transforming Growth Factor-β1 on the Cell Viability of Nasal Polyp-Derived Fibroblast

Ferryan Sofyan; Delfitri Munir; Imam Budi Putra; Retno Sulistyo Wardani; Restu Syamsul Hadi; Devira Zahara; Rosita Juwita Sembiring; Andrina Y. M. Rambe; Taufik Ashar

doi:10.3889/oamjms.2022.9516

Authors

Ferryan Sofyan Department of Otorhinolaryngology Head and Neck Surgery, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia; Philosophy Doctor In Medicine Programme, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia
Delfitri Munir Department of Otorhinolaryngology Head and Neck Surgery, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia; Philosophy Doctor In Medicine Programme, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia
Imam Budi Putra Department of Dermatology and Venereology, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia
Retno Sulistyo Wardani Department of Otorhinolaryngology Head and Neck Surgery, Faculty of Medicine, Universitas Indonesia, Dr. Cipto Mangunkusumo Hospital, Jakarta, Indonesia
Restu Syamsul Hadi Department of Anatomy, Faculty of Medicine, YARSI University, Jakarta, Indonesia
Devira Zahara Department of Otorhinolaryngology Head and Neck Surgery, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia
Rosita Juwita Sembiring Philosophy Doctor In Medicine Programme, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia; Department of Clinical Pathology, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia
Andrina Y. M. Rambe Department of Otorhinolaryngology Head and Neck Surgery, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia
Taufik Ashar Faculty of Public Health, Universitas Sumatra Utara, Medan, Indonesia

DOI:

https://doi.org/10.3889/oamjms.2022.9516

Keywords:

Thymoquinone, Transforming Growth Factor-β1, Nasal Polyp, Fibroblast

Abstract

BACKGROUND: Nasal polyps are benign masses in the nasal cavity and the abnormal growth of sinonasal tissue due to a chronic inflammatory process. Many fibroblasts populate the nasal polyp stroma release cytokines such as Transforming Growth Factor (TGF) and producing a variety of cytokines resulting in inflammatory cell infiltration. Thymoquinone (TQ) is the main active component in Nigella sativa oil and has the ability to reduces cell viability in many cancer cell line.

AIM: The purpose of this study was to determine the effect of TQ and TGF-β1 on cell viability of Nasal Polyp-Derived Fibroblast.

MATERIALS AND METHODS: Nasal polyp-derived fibroblasts were isolated from nasal polyp specimen and treated with various concentrations of TQ at 1–1000 μM and TGF-β1 at 5 ng/ml to determine the cell viability using the Cell Counting Kit-8 assay after 48 h incubation.

RESULTS: TQ significantly reduced the viability of nasal polyp fibroblast cells to 72.49% at 20 μM and reduced to 5% at 50 μM until 1000 μM with IC50 at 21.93 μM. TGF-β1 at 5 ng/ml significantly reduced the viability of nasal polyp fibroblast cells to 81.96% and TGF-β1 appears to have a dual effect that depends on the concentration of TQ.

CONCLUSION: This study proved that TQ and TGF-β1 were able to reduce the viability of nasal polyp fibroblast cells.

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