Analysis of Nitrosamines in Processed Meat Products in Medan City by Liquid Chromatography-Mass Spectrometry
DOI:
https://doi.org/10.3889/oamjms.2019.261Keywords:
Meat products, Nitrosamines, LC-MS/MSAbstract
BACKGROUND: Nitrosamine is a carcinogen and the maximum level in processed meat products set by WHO.
AIM: The purpose of this study was to determine nitrosamine levels in meat products in Medan City and compared nitrosamine levels with standards set by WHO.
METHODS: The samples analysed were obtained from Berastagi Supermarket, sausages, burgers, corned beef and smoked beef. Nitrosamine levels were determined by reverse phase liquid chromatography-mass spectrometry.
RESULTS: The results showed that only 5 out of 20 samples of N-nitroso-thiazolidine-4-carboxylic acid (NTCA) nitrosamine ranged from 501.290 to 4227.492 µg/kg. The highest level of NTCA was found in smoked beef (Chiefs), which is 4227.492 µg/kg. N-nitroso-2-methyl-thiazolidine-4-carboxylic acid (NMTCA) was contained in all the samples analysed which ranged from 20.50 to 989.175 µg/kg. The highest NMTCA nitrosamine content was found in smoked beef (Chiefs), which is 989.175 µg/kg.
CONCLUSION: From this study reveal that nitrosamines in meat products exceed the maximum standards set by WHO (10 µg/kg).
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References
Bryan NS, Loscalzo J. Introduction. Nitrite and Nitrate in Human Health and Disease. Humana Press. 2011:3-7. https://doi.org/10.1007/978-1-60761-616-0_1
Raczuk J, Wanda W, Katarzyna G. Nitrate and Nitrite in Select Vegetables Purchased at Supermarket in Siedlce. Journal of National Institute of Public Health-National Institute of Hygiene (NIPH-NIH) Poland. 2014; 65(1). PMid:24964574
Herrmann SS, Duedahl-Olesen L, Christensen T, Olesen PT, Granby K. Dietary exposure to volatile and non-volatile N-nitrosamines from processed meat products in Denmark. Food and Chemical Toxicology. 2015; 80:137-43. https://doi.org/10.1016/j.fct.2015.03.008 PMid:25792266
Silalahi J. Nitrite and nitrate problems in food, Medika Journal. 2005; 7(1).
Cintya H, Silalahi J, De Lux Putra E, Siburian R. The influence of storage condition on nitrite, nitrate and vitamin C levels in vegetables. F1000Research. 2018; 6(7):1899. https://doi.org/10.12688/f1000research.16853.1
Cintya H, Silalahi J, Lux Putra ED, Siburian R. The Influence of Fertilizer on Nitrate, Nitrite and Vitamin C Contents in Vegetables. Oriental Journal of Chemistry. 2018; 34(5):2614-21. https://doi.org/10.13005/ojc/340552
Al-Kaseem M, Al-Assaf Z, Karabeet F. A Rapid, Validated RP-HPLC Method for the Determination of Seven Volatile N-Nitrosamines in Meat. Pharmacology & Pharmacy. 2014; 5(03):298-308. https://doi.org/10.4236/pp.2014.53037
Yurchenko S, Mölder U. The occurrence of volatile N-nitrosamines in Estonian meat products. Food Chemistry. 2007; 100(4):1713-21. https://doi.org/10.1016/j.foodchem.2005.10.017
Domańska K, Kowalski B. 210 Effect of different storage conditions on nitrate/nitrite levels, microbiological quality and N-nitrosamines content in polish edible offals processed meat products. Toxicology Letters. 2003; 144:s60. https://doi.org/10.1016/S0378-4274(03)90209-8
Ogunmodede OT, Ojo AA, Jegede O. Comparative Study of Nitrosamine in Roasted Food Base on the Roasting Methods. International Letters of Natural Sciences. 2016; 50:23-6. https://doi.org/10.18052/www.scipress.com/ILNS.50.23
Li L, Wang P, Xu X, Zhou G. Influence of Various Cooking Methods on the Concentrations of Volatile N-Nitrosamines and Biogenic Amines in Dry-Cured Sausages. Journal of Food Science. 2012; 77(5):C560-C565. https://doi.org/10.1111/j.1750-3841.2012.02667.x PMid:23163937
Herrmann SS, Duedahl-Olesen L, Granby K. Occurrence of volatile and non-volatile N-nitrosamines in processed meat products and the role of heat treatment. Food Control. 2015; 48:163-9. https://doi.org/10.1016/j.foodcont.2014.05.030
Vanderstoep J. Nitrite Curing of Meat, the N-Nitrosamine Problem and Nitrite Alternatives. Food Research International. 2002; 35(4):411. https://doi.org/10.1016/S0963-9969(01)00128-4
Al-Kaseem M, Al-Assaf Z, Karabeet F. Determination of Seven Volatile N-Nitrosamines in Fast Food. Pharmacology & Pharmacy. 2014; 05(02):195-203. https://doi.org/10.4236/pp.2014.52026
Al-Kaseem M, Al-Assaf Z, Karabet F. Rapid and Simple Extraction Method for Volatile N-Nitrosamines in Meat Products. Pharmacology & Pharmacy. 2013; 4(08):611-8. https://doi.org/10.4236/pp.2013.48087
Yurchenko S, Mölder U. Volatile N-Nitrosamines in various fish products. Food Chemistry. 2006; 96(2):325-33. https://doi.org/10.1016/j.foodchem.2005.04.009
Sannino A, Bolzoni L. GC/CI-MS/MS method for the identification and quantification of volatile N-nitrosamines in meat products. Food Chemistry. 2013; 141(4):3925-30. https://doi.org/10.1016/j.foodchem.2013.06.070 PMid:23993567
Rywotycki R. The effect of selected functional additives and heat treatment on nitrosamine content in pasteurized pork ham. Meat Science. 2002; 60(4):335-9. https://doi.org/10.1016/S0309-1740(01)00138-3
Wang Y, Li F, Zhuang H, Chen X, Li L, Qiao W, et al. Effects of plant polyphenols and α-tocopherol on lipid oxidation, residual nitrites, biogenic amines, and N-nitrosamines formation during ripening and storage of dry-cured bacon. LWT - Food Science and Technology. 2015; 60(1):199-206. https://doi.org/10.1016/j.lwt.2014.09.022
Huang DP, Ho JHC, Webb KS, Wood BJ, Gough TA. Volatile nitrosamines in salt-preserved fish before and after cooking. Food and Cosmetics Toxicology. 1981; 19:167-71. https://doi.org/10.1016/0015-6264(81)90353-9
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Copyright (c) 2019 Henni Cintya, Jansen Silalahi, Effendy De Lux Putra, Rikson Siburian
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