The Comparison of RhoC and PI3K Gene Expression on the Breast Cancer Tissue and Benign Tumour Tissue

Authors

  • Dessy Arisanty Department of Biochemistry, Faculty of Medicine, Andalas University, Padang, Indonesia
  • Wirsma Arif Harahap Division of Surgical Oncology, Medical School of Dr. M. Djamil Hospital, Andalas University, Padang, Indonesia
  • Daan Khambri Division of Surgical Oncology, Medical School of Dr. M. Djamil Hospital, Andalas University, Padang, Indonesia
  • Rony Rustam Division of Surgical Oncology, Medical School of Dr. M. Djamil Hospital, Andalas University, Padang, Indonesia
  • Gestina Aliska Department Farmacology, Faculty of Medicine, Andalas University, Padang, Indonesia
  • Afifatul Achyar Biomedical Laboratory, Molecular Division, Faculty of Medicine, Andalas University, Padang, Indonesia
  • Juane Plantika Menra Biomedical Laboratory, Molecular Division, Faculty of Medicine, Andalas University, Padang, Indonesia

DOI:

https://doi.org/10.3889/oamjms.2019.543

Keywords:

RhoC gene, PI3K gene, Real-time PCR (qPCR), Cloning, Vector

Abstract

BACKGROUND: The expression of a gene is a process that conveys information of genes to synthesise gene product is functional. Alterations of the molecular biology in breast cancer are very complex because of many factors play a role in the tumorigenesis. RhoC is a prometastases gene. The PI3K gene is crucial in the regulation of multiple cellular functions, including cell growth, proliferation, metabolism and angiogenesis.
AIM: This study aims to compare of RhoC and PI3K gene expression on the breast cancer tissue and benign tumour tissue.
MATERIAL AND METHODS: Expression of the RhoC and PI3K genes was carried out with qPCR. The absolute quantification method was using breast cancer tissue. As a comparison, benign tumours (FATs) tissue was carried out. The standard curves were obtained from cloning target genes, which were inserted into the gGEMT-easy vector from E. coli. The gene expression data was carried out by t-test to see the mean difference between the expression of breast cancer tissue and benign tumours (FATs) with a value of p ≤ 0.005 in RhoC and PI3K gene expression. And the relationship between expressions was done by Pearson correlation test.
RESULTS: The results showed that it was found that PI3K gene expression on breast cancer tissue was higher than the number in a benign tumour (fibroadenoma mammae) as an endogenous control. And also, the expression of RhoC is much lower on breast cancer tissue compared with a benign tumour.
CONCLUSION: This study concluded that expression of RhoC affects the expression of PI3K so that the thing this is what causes the proliferation and began to provide support aggressive cancer cells in the breast.

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Published

2019-07-02

How to Cite

1.
Arisanty D, Harahap WA, Khambri D, Rustam R, Aliska G, Achyar A, Menra JP. The Comparison of RhoC and PI3K Gene Expression on the Breast Cancer Tissue and Benign Tumour Tissue. Open Access Maced J Med Sci [Internet]. 2019 Jul. 2 [cited 2024 Mar. 19];7(12):1911-6. Available from: https://oamjms.eu/index.php/mjms/article/view/oamjms.2019.543

Issue

Section

A - Basic Science

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